BRENDA - Enzyme Database

Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2

Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.; Microbiology 155, 2078-2085 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.1
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.1
expression in Escherichia coli; expression in Escherichia coli
Geobacillus thermodenitrificans
1.1.1.192
expression in Escherichia coli; expression in Escherichia coli
Geobacillus thermodenitrificans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
Al3+
1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.1
Co2+
1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Cu2+
1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
1.1.1.1
K+
1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mg2+
1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mn2+
1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Ni2+
1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
1.1.1.1
sodium dodecylsulfate
1 mM, 3% residual activity; 1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.1
Zn2+
1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Al3+
1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.192
Co2+
1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Cu2+
1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
1.1.1.192
K+
1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mg2+
1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mn2+
1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Ni2+
1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
1.1.1.192
sodium dodecylsulfate
1 mM, 3% residual activity; 1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.192
Zn2+
1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.28
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.4
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.51
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
2.25
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
4.71
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
7.55
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
37.6
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Fe2+
1 mM, 136% of initial activity; 1 mM, 168% of initial activity
Geobacillus thermodenitrificans
1.1.1.1
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases; enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
1.1.1.1
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
Fe2+
1 mM, 136% of initial activity; 1 mM, 168% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases; enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
1.1.1.192
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.1
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.1
90000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.1
380000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.192
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
90000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.192
380000
-
PAGE
Geobacillus thermodenitrificans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Geobacillus thermodenitrificans
A4IP64
-
-
1.1.1.1
Geobacillus thermodenitrificans
A4ISB9
-
-
1.1.1.1
Geobacillus thermodenitrificans NG80-2
A4IP64
-
-
1.1.1.1
Geobacillus thermodenitrificans NG80-2
A4ISB9
-
-
1.1.1.192
Geobacillus thermodenitrificans
A4IP64
-
-
1.1.1.192
Geobacillus thermodenitrificans
A4ISB9
-
-
1.1.1.192
Geobacillus thermodenitrificans NG80-2
A4IP64
-
-
1.1.1.192
Geobacillus thermodenitrificans NG80-2
A4ISB9
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
447
-
substrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 55% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 70% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 70% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 55% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans NG80-2
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans
ethanol + NAD+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans NG80-2
ethanol + NAD+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans
ethanol + NAD+ + H+
-
-
-
r
1.1.1.1
acetone + NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + NADH + H+
-
-
-
r
1.1.1.1
ethanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
ethanol + 2 NAD+ + H2O
about 45% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
ethanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.1
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isoamylalcohol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isopropanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
methanol + 2 NAD+ + H2o
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
formic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
1.1.1.1
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans NG80-2
?
-
-
-
-
1.1.1.1
octanal + NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
1.1.1.1
octanal + NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
octanoic acid + NADH + H+
-
-
-
r
1.1.1.1
octanoic acid + 2 NADH + 2 H+
-
712962
Geobacillus thermodenitrificans
1-octanol + 2 NAD+ + H2o
-
-
-
r
1.1.1.192
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-decanol + 2 NAD+ + H2O
about 20% of the activity with ethanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-decanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-dodecanol + 2 NAD+ + H2O
about 17% of the activity with ethanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-dodecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-eicosanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-eicosanol + 2 NAD+ + H2O
about 3% of the activity with ethanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-hexadecanol + 2 NAD+ + H2O
about 10% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-hexadecanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octacosanol + 2 NAD+ + H2O
about 40% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octacosanol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octadecanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octadecanol + 2 NAD+ + H2O
about 5% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octanol + 2 NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.192
1-tetracosanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetracosanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetradecanol + 2 NAD+ + H2O
about 15% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetradecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
decanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + NADH
-
-
-
r
1.1.1.192
dodecanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + NADH
-
-
-
r
1.1.1.192
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
1.1.1.192
octanal + NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.1
ADH1
-
Geobacillus thermodenitrificans
1.1.1.1
ADH2
-
Geobacillus thermodenitrificans
1.1.1.1
long-chain alkyl alcohol dehydrogenase
-
Geobacillus thermodenitrificans
1.1.1.192
ADH1
-
Geobacillus thermodenitrificans
1.1.1.192
ADH2
-
Geobacillus thermodenitrificans
1.1.1.192
long-chain alkyl alcohol dehydrogenase
-
Geobacillus thermodenitrificans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.1
60
-
;
Geobacillus thermodenitrificans
1.1.1.192
60
-
;
Geobacillus thermodenitrificans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.1
60
-
14 h, more than 50% of initial activity; 14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
60
-
14 h, more than 50% of initial activity; 14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
43.9
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
136.2
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
138.1
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
260.7
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
404.8
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
443.1
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
518.4
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
638.7
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1646
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
8
-
;
Geobacillus thermodenitrificans
1.1.1.192
8
-
;
Geobacillus thermodenitrificans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
6
9
;
Geobacillus thermodenitrificans
1.1.1.192
6
9
;
Geobacillus thermodenitrificans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.1
NADH
-
Geobacillus thermodenitrificans
1.1.1.1
NADP+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.1
NADPH
-
Geobacillus thermodenitrificans
1.1.1.192
NAD+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.192
NADH
-
Geobacillus thermodenitrificans
1.1.1.192
NADP+
NAD+ is prefered over NADP+; NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.192
NADPH
-
Geobacillus thermodenitrificans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.1
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
EDTA
1 mM, 105% of initial activity
Geobacillus thermodenitrificans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
expression in Escherichia coli
Geobacillus thermodenitrificans
1.1.1.192
expression in Escherichia coli
Geobacillus thermodenitrificans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
1.1.1.1
NAD+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.1
NADH
-
Geobacillus thermodenitrificans
1.1.1.1
NADP+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
1.1.1.1
NADP+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.1
NADPH
-
Geobacillus thermodenitrificans
1.1.1.192
NAD+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
1.1.1.192
NAD+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.192
NADH
-
Geobacillus thermodenitrificans
1.1.1.192
NADP+
NAD+ is prefered over NADP+
Geobacillus thermodenitrificans
1.1.1.192
NADP+
NADP+ is prefered over NAD+
Geobacillus thermodenitrificans
1.1.1.192
NADPH
-
Geobacillus thermodenitrificans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
Al3+
1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Al3+
1 mM, 14.7% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.1
Co2+
1 mM, 51.9% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Co2+
1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Cu2+
1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Cu2+
1 mM, 14.0% residual activity
Geobacillus thermodenitrificans
1.1.1.1
K+
1 mM, 70.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
K+
1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mg2+
1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mg2+
1 mM, 0.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mn2+
1 mM, 14.3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Mn2+
1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Ni2+
1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Ni2+
1 mM, 18.2% residual activity
Geobacillus thermodenitrificans
1.1.1.1
sodium dodecylsulfate
1 mM, 3% residual activity
Geobacillus thermodenitrificans
1.1.1.1
sodium dodecylsulfate
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.1
Zn2+
1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
1.1.1.1
Zn2+
1 mM, 14.1% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Al3+
1 mM, 18.9% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Al3+
1 mM, 14.7% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Ca2+
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.192
Co2+
1 mM, 51.9% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Co2+
1 mM, 68.7% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Cu2+
1 mM, 44.2% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Cu2+
1 mM, 14.0% residual activity
Geobacillus thermodenitrificans
1.1.1.192
K+
1 mM, 70.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
K+
1 mM, 82.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mg2+
1 mM, 74.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mg2+
1 mM, 0.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mn2+
1 mM, 14.3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Mn2+
1 mM, 23.4% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Ni2+
1 mM, 72.7% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Ni2+
1 mM, 18.2% residual activity
Geobacillus thermodenitrificans
1.1.1.192
sodium dodecylsulfate
1 mM, 3% residual activity
Geobacillus thermodenitrificans
1.1.1.192
sodium dodecylsulfate
1 mM, no residual activity
Geobacillus thermodenitrificans
1.1.1.192
Zn2+
1 mM, 30.5% residual activity
Geobacillus thermodenitrificans
1.1.1.192
Zn2+
1 mM, 14.1% residual activity
Geobacillus thermodenitrificans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.28
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.4
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1.51
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
2.25
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
4.71
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
7.55
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
37.6
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
0.84
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
1.15
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
1.44
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
2.4
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
3.88
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Fe2+
1 mM, 136% of initial activity
Geobacillus thermodenitrificans
1.1.1.1
Fe2+
1 mM, 168% of initial activity
Geobacillus thermodenitrificans
1.1.1.1
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
1.1.1.1
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
Fe2+
1 mM, 136% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
Fe2+
1 mM, 168% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
additional information
enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases
Geobacillus thermodenitrificans
1.1.1.192
Na+
1 mM, 110% of initial activity
Geobacillus thermodenitrificans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.1
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.1
90000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.1
380000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.192
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
90000
-
PAGE
Geobacillus thermodenitrificans
1.1.1.192
380000
-
PAGE
Geobacillus thermodenitrificans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
447
-
substrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-butanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
butanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 55% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 70% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 70% of the activity with ethanol
712962
Geobacillus thermodenitrificans NG80-2
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-hexanol + 2 NAD+ + H2O
about 55% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
hexanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans NG80-2
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans
ethanol + NAD+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans NG80-2
ethanol + NAD+
-
-
-
r
1.1.1.1
acetaldehyde + NADH + H+
-
712962
Geobacillus thermodenitrificans
ethanol + NAD+ + H+
-
-
-
r
1.1.1.1
acetone + NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + NADH + H+
-
-
-
r
1.1.1.1
ethanol + 2 NAD+ + H2O
best substrate
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
ethanol + 2 NAD+ + H2O
about 45% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
ethanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
acetic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.1
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isoamylalcohol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isopropanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
methanol + 2 NAD+ + H2o
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
formic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.1
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
1.1.1.1
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans NG80-2
?
-
-
-
-
1.1.1.1
octanal + NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
1.1.1.1
octanal + NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans NG80-2
octanoic acid + NADH + H+
-
-
-
r
1.1.1.1
octanoic acid + 2 NADH + 2 H+
-
712962
Geobacillus thermodenitrificans
1-octanol + 2 NAD+ + H2o
-
-
-
r
1.1.1.192
1,3-propanediol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1,3-propanediol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-decanol + 2 NAD+ + H2O
about 20% of the activity with ethanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-decanol + 2 NAD+ + H2O
about 50% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-dodecanol + 2 NAD+ + H2O
about 17% of the activity with ethanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-dodecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-eicosanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-eicosanol + 2 NAD+ + H2O
about 3% of the activity with ethanol
712962
Geobacillus thermodenitrificans
eicosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-hexadecanol + 2 NAD+ + H2O
about 10% of the activity with ethanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-hexadecanol + 2 NAD+ + H2O
about 25% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
hexadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octacosanol + 2 NAD+ + H2O
about 40% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octacosanol + 2 NAD+ + H2O
about 5% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octacosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octadecanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octadecanol + 2 NAD+ + H2O
about 5% of the activity with ethanol
712962
Geobacillus thermodenitrificans
octadecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octanol + 2 NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-octanol + 2 NADP+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + 2 NADPH + 2 H+
-
-
-
r
1.1.1.192
1-tetracosanol + 2 NAD+ + H2O
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetracosanol + 2 NAD+ + H2O
about 30% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetracosanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetradecanol + 2 NAD+ + H2O
about 15% of the activity with ethanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
1-tetradecanol + 2 NAD+ + H2O
about 30% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
tetradecanoic acid + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
decanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
decanoic acid + NADH
-
-
-
r
1.1.1.192
dodecanal + NAD+ + H+
about 10% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
dodecanoic acid + NADH
-
-
-
r
1.1.1.192
glycerol + 2 NAD+ + H2O
about 45% of the activity with ethanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
isopropanol + 2 NAD+ + H2O
about 15% of the activity with 1-octanol
712962
Geobacillus thermodenitrificans
? + 2 NADH + 2 H+
-
-
-
r
1.1.1.192
additional information
substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol
712962
Geobacillus thermodenitrificans
?
-
-
-
-
1.1.1.192
octanal + NAD+ + H2O
-
712962
Geobacillus thermodenitrificans
octanoic acid + NADH + H+
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
1.1.1.192
octamer
8 * 45000, SDS-PAGE, 8 * 41664, calculated
Geobacillus thermodenitrificans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.1
60
-
-
Geobacillus thermodenitrificans
1.1.1.192
60
-
-
Geobacillus thermodenitrificans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.1
60
-
14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
1.1.1.192
60
-
14 h, more than 50% of initial activity
Geobacillus thermodenitrificans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
43.9
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
136.2
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
138.1
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
260.7
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
404.8
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
443.1
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
518.4
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
638.7
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1646
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
38.5
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
283.1
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
4808
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
8
-
-
Geobacillus thermodenitrificans
1.1.1.192
8
-
-
Geobacillus thermodenitrificans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
6
9
-
Geobacillus thermodenitrificans
1.1.1.192
6
9
-
Geobacillus thermodenitrificans
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
3.6
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
61.4
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
84.6
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
85.9
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
156.8
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
293.4
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1176
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
3.6
-
ethanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
61.4
-
acetaldehyde
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
84.6
-
ethanol
cosubstrate NAD+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
85.9
-
acetaldehyde
cosubstrate NADH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
156.8
-
NADP+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
293.4
-
NAD+
cosubstrate ethanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
1176
-
NADH
cosubstrate acetaldehyde, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.1
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
26.7
-
NAD+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
67.2
-
1-Octanol
cosubstrate NADP+, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
117.9
-
NADP+
cosubstrate 1-octanol, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
450.8
-
octanal
cosubstrate NADPH, pH 8.0, 60°C
Geobacillus thermodenitrificans
1.1.1.192
5724
-
NADPH
cosubstrate octanal, pH 8.0, 60°C
Geobacillus thermodenitrificans