Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eltelib, H.A.; Badejo, A.A.; Fujikawa, Y.; Esaka, M.
    Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra) (2011), J. Plant Physiol., 168, 619-627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 gene encoding DHAr, DNA and amino acid sequence determination and analysis, phylogenetic tree Malpighia glabra

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.1 cytosol
-
 Malpighia glabra 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.1 2 glutathione + dehydroascorbate Malpighia glabra
-
 glutathione disulfide + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Malpighia glabra E2RWY5 acerola
-
1.8.5.1 Malpighia glabra E2RWY6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 fruit MDHAR activity increases gradually and significantly as ripening progresses,with overripe fruits having the highest activity Malpighia glabra
-
1.6.5.4 leaf
-
 Malpighia glabra
-
1.8.5.1 fruit ripening, expression analysis, transcript level of DHAR is highest at the intermediate stage of fruit ripening, overview Malpighia glabra
-
1.8.5.1 leaf
-
 Malpighia glabra
-
1.8.5.1 additional information under dark conditions, there is a sharp and significant decline in the total and reduced ascorbate contents, accompanied by a decrease in the level of transcripts and enzyme activities of the two genes in acerola leaves Malpighia glabra
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NADH + monodehydroascorbate
-
 Malpighia glabra NAD+ + ascorbate
-
 ?
1.8.5.1 2 glutathione + dehydroascorbate
-
 Malpighia glabra glutathione disulfide + ascorbate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDHAR
-
 Malpighia glabra
1.8.5.1 dehydroascorbate reductase
-
 Malpighia glabra
1.8.5.1 DHAR
-
 Malpighia glabra

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.5.1 25
-
 assay at Malpighia glabra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 7
-
 assay at Malpighia glabra

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Malpighia glabra

Expression

EC Number Organism Comment Expression
1.6.5.4 Malpighia glabra MDHAR transcripts and enzyme activities are significantly up-regulated in the leaves of acerola under cold (4°C for 24 h) and salt (treatment with 150 mM NaCl over 9 days) stress conditions. MDHAR activity increases gradually and significantly as ripening progresses,with overripe fruits having the highest activity up
1.8.5.1 Malpighia glabra under dark conditions, there is a sharp and significant decline in the total and reduced ascorbate contents, accompanied by a decrease in the level of transcripts and enzyme activities of the two genes in acerola leaves down
1.8.5.1 Malpighia glabra DHAR transcript and enzyme activity are significantly up-regulated in the leaves of acerola under cold and salt stress conditions up

General Information

EC Number General Information Comment Organism
1.8.5.1 additional information transcriptional regulation of DHAR, overview Malpighia glabra
1.8.5.1 physiological function monodehydroascorbate reductase and dehydroascorbate reductase are key enzymes of the ascorbate-glutathione cycle that maintain reduced pools of ascorbic acid and serve as important antioxidants Malpighia glabra