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Literature summary extracted from

  • Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.
    Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus (2010), J. Plant Physiol., 168, 549-557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.103 DNA and amino acid sequence determination and analysis Catharanthus roseus
2.1.1.94
-
 Catharanthus roseus
2.3.1.107 transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker Catharanthus roseus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.107 additional information analysis of interaction of vindoline biosynthetic enzymes including DAT using yeast two-hybrid assays, overview. DAT does not interact with the other enzymes Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.20 additional information vindoline biosynthesis pathway enzyme subcellular localization study, overview Catharanthus roseus
-
-
1.14.11.20 nucleoplasm the enzyme resides in the nucleocytoplasmic compartment following passive diffusion to the nucleus allowed by the protein size. Colocalization with deacetylvindoline-4-O-acetyltransferase Catharanthus roseus 5654
-
1.14.14.103 endoplasmic reticulum
-
 Catharanthus roseus 5783
-
1.14.14.103 endoplasmic reticulum membrane T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix Catharanthus roseus 5789
-
1.14.14.103 additional information vindoline biosynthesis pathway enzyme subcellular localization study, overview Catharanthus roseus
-
-
2.1.1.94 cytoplasm
-
 Catharanthus roseus 5737
-
2.1.1.94 cytosol
-
 Catharanthus roseus 5829
-
2.3.1.107 cytoplasm
-
 Catharanthus roseus 5737
-
2.3.1.107 additional information GFP-tagged DAT is localized to the cytoplasm and the nucleus Catharanthus roseus
-
-
2.3.1.107 nucleus
-
 Catharanthus roseus 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.20 deacetoxyvindoline + 2-oxoglutarate + O2 Catharanthus roseus
-
 deacetylvindoline + succinate + CO2
-
 ?
1.14.11.20 additional information Catharanthus roseus the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system ?
-
 ?
1.14.14.103 tabersonine + NADPH + H+ + O2 Catharanthus roseus
-
 16-hydroxytabersonine + NADP+ + H2O
-
 ?
2.3.1.107 acetyl-CoA + deacetylvindoline Catharanthus roseus
-
 CoA + vindoline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.20 Catharanthus roseus
-
-
-
1.14.14.103 Catharanthus roseus C0KYN4
-
-
2.1.1.94 Catharanthus roseus B0EXJ8
-
-
2.3.1.107 Catharanthus roseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.20 idioblast
-
 Catharanthus roseus
-
1.14.11.20 laticifer
-
 Catharanthus roseus
-
1.14.11.20 leaf
-
 Catharanthus roseus
-
1.14.14.103 leaf
-
 Catharanthus roseus
-
2.1.1.94 cotyledon
-
 Catharanthus roseus
-
2.1.1.94 epidermis specifically located in the epidermis of aerial organs Catharanthus roseus
-
2.1.1.94 leaf
-
 Catharanthus roseus
-
2.3.1.107 leaf
-
 Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.20 deacetoxyvindoline + 2-oxoglutarate + O2
-
 Catharanthus roseus deacetylvindoline + succinate + CO2
-
 ?
1.14.11.20 additional information the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system Catharanthus roseus ?
-
 ?
1.14.14.103 tabersonine + NADPH + H+ + O2
-
 Catharanthus roseus 16-hydroxytabersonine + NADP+ + H2O
-
 ?
2.1.1.94 S-adenosyl-L-methionine + 16-hydroxytabersonine
-
 Catharanthus roseus S-adenosyl-L-homocysteine + 16-methoxytabersonine
-
 ?
2.3.1.107 acetyl-CoA + deacetylvindoline
-
 Catharanthus roseus CoA + vindoline
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.20 monomer
-
 Catharanthus roseus
1.14.14.103 monomer
-
 Catharanthus roseus
2.1.1.94 homodimer
-
 Catharanthus roseus
2.3.1.107 More the enzyme is no hetero- or homodimer Catharanthus roseus

Synonyms

EC Number Synonyms Comment Organism
1.14.11.20 D4H
-
 Catharanthus roseus
1.14.11.20 desacetoxyvindoline-4-hydroxylase
-
 Catharanthus roseus
1.14.14.103 CYP71D12
-
 Catharanthus roseus
1.14.14.103 T16H
-
 Catharanthus roseus
1.14.14.103 tabersonine 16-monooxygenase
-
 Catharanthus roseus
2.1.1.94 16-hydroxytabersonine 16-O-methyltransferase
-
 Catharanthus roseus
2.1.1.94 16OMT
-
 Catharanthus roseus
2.3.1.107 DAT
-
 Catharanthus roseus
2.3.1.107 deacetylvindoline-4-O-acetyltransferase
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.103 cytochrome P450
-
 Catharanthus roseus
2.3.1.107 acetyl-CoA
-
 Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.14.11.20 metabolism the enzyme catalyzes the penultimate step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. The desacetoxyvindoline-4-hydroxylase interacts directly with the deacetylvindoline-4-O-acetyltransferase, that catalyzes the last step of the biosynthetic pathway, absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview Catharanthus roseus
1.14.14.103 metabolism the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview Catharanthus roseus
2.3.1.107 metabolism vindoline constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial tabersonine-to-vindoline biosynthesis organization involving four enzymes for the six-step conversion, overview. Deacetylvindoline-4-O-acetyltransferase catalyzes teh last step of the pathway Catharanthus roseus