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Literature summary extracted from

  • Li, M.; Zhang, G.; Wang, H.; Ng, T.
    Purification and characterization of a laccase from the edible wild mushroom Tricholoma mongolicum (2010), J. Microbiol. Biotechnol., 20, 1069-1076.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ weak inhibition Leucocalocybe mongolica
1.10.3.2 Fe2+ weak inhibition Leucocalocybe mongolica
1.10.3.2 Hg2+ strong inhibition Leucocalocybe mongolica
1.10.3.2 Pb2+ weak inhibition Leucocalocybe mongolica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0023
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in 67 mM sodium acetate buffer (pH 4.5), at 30°C Leucocalocybe mongolica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ the enzyme is strongly stimulated by Cu2+ Leucocalocybe mongolica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 66000
-
 1 * 66000, SDS-PAGE Leucocalocybe mongolica

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Leucocalocybe mongolica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 DEAE-cellulose column chromatography, CM-cellulose column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration Leucocalocybe mongolica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
 the specific activity of the crude enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 11.3 units/mg, the specific activity of the 131fold purification enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 1480 units/mg. One unit of enzyme activity is defined as the amount of enzyme required to produce an absorbance increase at 420 nm of 1 per minute per ml of reaction mixture Leucocalocybe mongolica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 100% activity Leucocalocybe mongolica ?
-
 ?
1.10.3.2 2-methoxyphenol + O2 8.6% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Leucocalocybe mongolica ?
-
 ?
1.10.3.2 catechol + O2 11.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Leucocalocybe mongolica ?
-
 ?
1.10.3.2 hydroquinone + O2 13.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Leucocalocybe mongolica ?
-
 ?
1.10.3.2 additional information no activity with L-tyrosine Leucocalocybe mongolica ?
-
 ?
1.10.3.2 N,N-dimethyl-1,4-phenylenediamine + O2 49.4% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Leucocalocybe mongolica ?
-
 ?
1.10.3.2 pyrogallol + O2 3.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Leucocalocybe mongolica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 66000, SDS-PAGE Leucocalocybe mongolica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 30
-
 purified enzyme Leucocalocybe mongolica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 5 70 the enzyme activity increases by about 70% to its maximum at 30°C when the temperature is elevated from 5°C, and then falls to about 10% of the maximal value when the temperature is raised to 70°C Leucocalocybe mongolica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 1480
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in 67 mM sodium acetate buffer (pH 4.5), at 30°C Leucocalocybe mongolica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2 3 purified enzyme Leucocalocybe mongolica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 8 the activity rises abruptly when the pH is increased from slightly below 2.0. The activity declines sharply when the pH is raised from 4.0 to 5.0 and is indiscernible at pH 6.0-8.0 Leucocalocybe mongolica

General Information

EC Number General Information Comment Organism
1.10.3.2 physiological function laccase inhibits HIV-1 reverse transcriptase and proliferation of hepatoma Hep-G2 cells and breast cancer MCF-7 cells with an IC50 of 0.00065 mM, 0.0014 mM, and 0.0042 mM, respectively, indicating that it is also an antipathogenic protein Leucocalocybe mongolica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 643500
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in 67 mM sodium acetate buffer (pH 4.5), at 30°C Leucocalocybe mongolica