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Literature summary extracted from

  • Ni Eidhin, D.; Degn, P.; Obeirne, D.
    Characterization of polyphenol oxidase from rooster potato (Solanum tuberosum cv Rooster) (2010), J. Food Biochem., 34, 13-30.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 SDS 172% activity at 10 mM Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 ascorbic acid effective inhibitor, 99% inhibition at 1 mM Solanum tuberosum
1.14.18.1 benzoic acid 9% residual activity at 10 mM Solanum tuberosum
1.14.18.1 borax 71% residual activity at 10 mM Solanum tuberosum
1.14.18.1 CaCl2 69% residual activity at 10 mM Solanum tuberosum
1.14.18.1 Citric acid 10% residual activity at 10 mM Solanum tuberosum
1.14.18.1 EDTA 57% residual activity at 10 mM Solanum tuberosum
1.14.18.1 L-cysteine complete inhibition at 1 mM Solanum tuberosum
1.14.18.1 N-acetyl-L-cysteine 1% residual activity at 1 mM Solanum tuberosum
1.14.18.1 NaCl 84% residual activity at 10 mM Solanum tuberosum
1.14.18.1 potassium sorbate 78% residual activity at 10 mM Solanum tuberosum
1.14.18.1 Sodium metabisulfite complete inhibition at 1 mM; effective inhibitor, complete inhibition at 1 mM Solanum tuberosum
1.14.18.1 succinic acid 35% residual activity at 10 mM Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 additional information Solanum tuberosum the partially purified enzyme has both cresolase and catecholase activity. Activity is lower toward monophenols than diphenols ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Solanum tuberosum
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cultivar Rooster, rooster potato
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate precipitation, phenyl Sepharose column chromatography, ion exchange chromatography, and hydroxyapatite column chromatography Solanum tuberosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
the specific activity of the crude enzyme is 264 units/mg protein, the specific activity of the 64fold purified enzyme is16853 units/mg protein using 50 mM catechol as substrate in 50 mM sodium phosphate buffer, pH 7.0, at 25°C, one unit of PPO activity is defined as the change in absorbance of 0.001 per minute per ml of enzyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 (-)-epicatechin + O2 119% activity at 2.5 mM substrate concentration Solanum tuberosum ?
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?
1.14.18.1 2 catechol + O2 100% activity at 10 mM substrate concentration Solanum tuberosum 2 1,2-benzoquinone + 2 H2O
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?
1.14.18.1 4-methylcatechol + O2 highest activity, 183% activity at 10 mM substrate concentration Solanum tuberosum 4-methyl-1,2-benzoquinone + H2O
-
?
1.14.18.1 caffeic acid + O2 31% activity at 2.5 mM substrate concentration Solanum tuberosum caffeoyl quinone + H2O
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?
1.14.18.1 DL-DOPA + O2 39% activity at 2.5 mM substrate concentration Solanum tuberosum dopaquinone + H2O
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?
1.14.18.1 L-tyrosine + O2 very low activity, 6% activity at 2.5 mM substrate concentration Solanum tuberosum L-DOPA + H2O
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?
1.14.18.1 additional information the partially purified enzyme has both cresolase and catecholase activity. Activity is lower toward monophenols than diphenols Solanum tuberosum ?
-
?
1.14.18.1 additional information no activity with ferulic acid and phenol Solanum tuberosum ?
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?
1.14.18.1 p-cresol + O2 20% activity at 2.5 mM substrate concentration Solanum tuberosum ?
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?
1.14.18.1 pyrogallol + O2 92% activity at 10 mM substrate concentration Solanum tuberosum ?
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?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
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Solanum tuberosum
1.14.18.1 PPO
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Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 30
-
-
Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 20 80 residual activity is higher than 50% after incubation at 20°C for 72 h, at 30°C for 48 h, at 40°C for 24 h, at 50°C for 2 h, and at 60°C for 15 min. Heating at 80°C for 15 min inactivates the enzyme Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6 6.5
-
Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 4.5 7.5
-
Solanum tuberosum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 2.5 3.5 the enzyme is most unstable at pH 2.5-3.5 retaining less than 10% of original activity after 2 h Solanum tuberosum
1.14.18.1 6 7.5 more than 50% activity is retained during storage for 72 h at pH 6.0-7.5 Solanum tuberosum