EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 1,3-dimethylimidazolium methylsulfate | 69.7% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | 1-butyl-3-methylimidazolium methylsulfate | 47.8% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | 1-ethyl-3-methylimidazolium methylsulfate | 64.1% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | acetone | increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in acetone for about 34 h | Agaricus bisporus | |
1.14.18.1 | chloroform | the cross-linked enzyme aggregate shows about 30% residual activity after incubation in chloroform for about 3 h | Agaricus bisporus | |
1.14.18.1 | choline acetate | 27.9% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | choline dihydrophosphate | 27.4% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | choline methylsulfonate | 39.7% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | choline nitrate | 54.6% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | dioxane | increasing solvent concentration up to 80% (v/v) yields a gradual reduction in the activity of the soluble and cross-linked enzyme forms, the cross-linked enzyme aggregate shows about 40% residual activity after incubation in dioxane for about 62 h | Agaricus bisporus | |
1.14.18.1 | EDTA | - |
Agaricus bisporus | |
1.14.18.1 | Guanidine-HCl | treatment with guanidine-HCl at increasing concentrations (0-800 mM) results in a reduced activity for both enzyme forms, but aggregation as cross-linked enzyme aggregate improves tyrosinase stability at higher concentrations (above 314 mM) | Agaricus bisporus | |
1.14.18.1 | hexane | the cross-linked enzyme aggregate shows about 20% residual activity after incubation in hexane for about 24 h | Agaricus bisporus | |
1.14.18.1 | methanol | the soluble enzyme retains 7.8% of its original activity, as compared to 31% by the cross-linked enzyme aggregates, after being incubated in the presence of 40% (v/v) methanol | Agaricus bisporus | |
1.14.18.1 | NaN3 | treatment with NaN3 at increasing concentrations (0-4 mM) results in a reduced activity for both soluble and cross-linked enzyme forms, but aggregation as cross-linked enzyme aggregates improves tyrosinase stability at higher concentrations (above 0.4 mM) | Agaricus bisporus | |
1.14.18.1 | SDS | a sharp decrease in the activity of the soluble enzyme is noted from 0 to 17 mM SDS | Agaricus bisporus | |
1.14.18.1 | tert-butanol | the cross-linked enzyme aggregate shows about 50% residual activity after incubation in tert-butanol for about 326 h | Agaricus bisporus | |
1.14.18.1 | tetrabutylammonium acetate | 24.1% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | tetrabutylammonium methylsulfonate | 45.3% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | tetramethylammonium acetate | 30% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | tributylammonium dihydrophosphate | 27.5% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | triethylammonium dihydrophosphate | 23.4% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | trimethylammonium dihydrophosphate | 12% residual activity at 5% (w/v) | Agaricus bisporus | |
1.14.18.1 | trimethylammonium methylsulfonate | 40.9% residual activity at 5% (w/v) | Agaricus bisporus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.41 | - |
L-Dopa | soluble enzyme, pH 6.0, 55°C | Agaricus bisporus | |
1.14.18.1 | 1.16 | - |
L-Dopa | cross-linked enzyme aggregate, pH 6.0, 30°C | Agaricus bisporus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | Cu2+ | the enzyme contains copper | Agaricus bisporus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Agaricus bisporus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | L-DOPA + O2 | - |
Agaricus bisporus | dopaquinone + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | tyrosinase | - |
Agaricus bisporus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 30 | 40 | cross-linked enzyme aggregate | Agaricus bisporus |
1.14.18.1 | 55 | - |
soluble enzyme | Agaricus bisporus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 40 | 60 | the soluble enzyme shows half-lives of about 120 min, 30 min, and 10 min at 40, 50, and 60°C, respectively. The cross-linked enzyme aggregates show half-lives of about 320 min, 120 min, and 30 min at 40, 50, and 60°C, respectively | Agaricus bisporus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 6 | - |
- |
Agaricus bisporus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 5 | 8 | the soluble enzyme shows half-lives of about 11 min, 35 min, and 3 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively. The cross-linked enzyme aggregates show half-lives of about 11 min, 35 min, and 1 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively | Agaricus bisporus |