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Literature summary extracted from

  • Kim, D.H.; Kim, B.G.; Lee, Y.; Ryu, J.Y.; Lim, Y.; Hur, H.G.; Ahn, J.H.
    Regiospecific methylation of naringenin to ponciretin by soybean O-methyltransferase expressed in Escherichia coli (2005), J. Biotechnol., 119, 155-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.231 expressed in Escherichia coli as a glutathione S-transferase fusion protein. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.231 Glycine max
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.231 additional information SOMT-2 has a regiospecific methylation activity, resulting in transforming 4'-hydroxyl group of flavonoids B-ring to 4'-methyl group. Caffeic acid, catechol, ferulic acid, and oricinol are biotransformed at less than 1% Glycine max ?
-
?
2.1.1.231 S-adenosyl-L-methionine + apigenin 36% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + acacetin
-
?
2.1.1.231 S-adenosyl-L-methionine + daidzein 90% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + formononetin
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?
2.1.1.231 S-adenosyl-L-methionine + genistein 62% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + biochanin A
-
?
2.1.1.231 S-adenosyl-L-methionine + naringenin i.e. 4',5,7-trihyroxyflavanone. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + ponciretin i.e. 4'-methoxy-5,7-dihydroxyflavanone ?
2.1.1.231 S-adenosyl-L-methionine + quercetin Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + 4'-methylquercetin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.231 SOMT-2
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Glycine max