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Literature summary extracted from

  • Chiang, B.Y.; Chen, T.C.; Pai, C.H.; Chou, C.C.; Chen, H.H.; Ko, T.P.; Hsu, W.H.; Chang, C.Y.; Wu, W.F.; Wang, A.H.; Lin, C.H.
    Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetase/amidase in redox regulation (2010), J. Biol. Chem., 285, 25345-25353.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.78 H2O2 50% inhibition with 0.25 mM. Inactivation is above 95% with 0.5 mM H2O2 for 5 min. Cysteine thiol of the GspSA amidase active-site nucleophile Cys59 is transiently inactivated by H2O2 oxidation to sulfenic acid which leads to an accumulation of glutathionylspermidine and an increased level of glutathionylspermidine S-thiolated proteins after oxidative stress. The hypersensitivities of GspSA and GspSA/glutaredoxin null mutants to H2O2 support the idea that GspSA and glutaredoxin act synergistically to regulate the redox environment of Escherichia coli Escherichia coli K-12
6.3.1.8 additional information H2O2 leads to inhibition of the amidase activity, while the glutathionylspermidine synthase activity is almost unaffected Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.78 Escherichia coli K-12 P0AES0
-
-
6.3.1.8 Escherichia coli P0AES0 bifunctional glutathionylspermidine synthetase/amidase
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.78 H2O2-mediated inactivation of the amidase activity can be recovered by the addition of 4 mM glutathione. ca. 80% of the enzyme activity are rescued Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.78 glutathionylspermidine + H2O
-
Escherichia coli K-12 glutathione + spermidine
-
?
3.5.1.78 glutathionylspermidine disulfide + H2O a coupled reaction of Gsp amidase and glutathione reductase Escherichia coli K-12 glutathione + ? products of the coupled reaction of Gsp amidase and glutathione reductase: spermidine, glutathionylspermidine-glutathione mixed disulfide, and glutathione disulfide are generated ?
3.5.1.78 additional information glutathionylspermidine S-thiolated peptides and proteins are tested as substrates. The protein substrates are treated with disulfide or biotinated glutathionylspermidine to generate biotin-labeled glutathionylspermidine S-thiolated proteins for the detection with an anti-biotin antibody after the reaction with the GspSA. A variety of glutathionylspermidine-derivatized substrates are hydrolyzed yielding spermidine and glutathione S-thiolated protein/peptides Escherichia coli K-12 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.78 GSP amidase
-
Escherichia coli K-12
3.5.1.78 Gsp synthetase/amidase bifunctional enzyme, EC 3.5.1.78 and EC 6.3.1.8 Escherichia coli K-12
3.5.1.78 GspSA
-
Escherichia coli K-12
6.3.1.8 GspSA
-
Escherichia coli

General Information

EC Number General Information Comment Organism
6.3.1.8 physiological function hypersensitivities of the GspSA/glutaredoxin null mutants to H2O2 support the idea that GspSA and Grx synergistically defend against oxidative damage Escherichia coli