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Literature summary extracted from

  • Guevara, T.; Yiallouros, I.; Kappelhoff, R.; Bissdorf, S.; Stoecker, W.; Gomis-Rueth, F.X.
    Proenzyme structure and activation of astacin metallopeptidase (2010), J. Biol. Chem., 285, 13958-13965.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.21 expressed in Escherichia coli BL21(DE3) cells Astacus astacus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.21 proastacin is crystallized by the sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 0.05 M MES, pH 5.6 Astacus astacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.21 Zn2+ required for activity Astacus astacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.21 Collagen + H2O Astacus astacus
-
 ?
-
 ?
3.4.24.21 Gelatin + H2O Astacus astacus
-
 ?
-
 ?
3.4.24.21 additional information Astacus astacus optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.21 Astacus astacus Q9U918 European crayfish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.21 proastacin is purified by Ni-NTA column chromatography and HEMA-Bio gel filtration Astacus astacus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.21 midgut gland astacin is synthesized as a preproenzyme in the midgut gland Astacus astacus
-
3.4.24.21 pancreatic duct the proenzyme is only transiently found within the ducts between the hepatopancreas and the stomach Astacus astacus
-
3.4.24.21 stomach mature enzyme Astacus astacus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.21 Collagen + H2O
-
 Astacus astacus ?
-
 ?
3.4.24.21 Gelatin + H2O
-
 Astacus astacus ?
-
 ?
3.4.24.21 additional information optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 Astacus astacus ?
-
 ?