EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.1.4 | expressed in Escherichia coli BL21(DE3) pLys strain | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.1.4 | E585A | the mutated amino acids are key residues that are involved in the mediation of structural changes in the head that follow the hydrolysis of the nucelotide. The mutant retains the basal, very low ATPase activity but shows negligible microtubule-stimulated ATPase | Drosophila melanogaster |
5.6.1.4 | E585D | the mutated amino acids are key residues that are involved in the mediation of structural changes in the head that follow the hydrolysis of the nucelotide. The mutant retains the basal, very low ATPase activity but shows negligible microtubule-stimulated ATPase | Drosophila melanogaster |
5.6.1.4 | additional information | a deletion in the motor domain in one of the subunits resulting in a single-headed molecule, NcN, resulting in a low affinity for microtubules. Mutated homodimers have no microtubule-activated ATPase and no motility, whereas NcN have motility comparable with that of the wild-type Ncd | Drosophila melanogaster |
5.6.1.4 | N600K | a decoupling mutant, Asn600 is essential for integrity of the Ncd dimeric structure and transmission of conformational changes from the switch region to the microtubule-binding site. The mutant retains the basal, very low ATPase activity but shows negligible microtubule-stimulated ATPase | Drosophila melanogaster |
5.6.1.4 | R552A | the mutated amino acids are key residues that are involved in the mediation of structural changes in the head that follow the hydrolysis of the nucelotide. The mutant retains the basal, very low ATPase activity but shows negligible microtubule-stimulated ATPase | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.4 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.1.4 | mutated protein molecules carrying point mutations in one of the heads, thus producing heterodimeric motors | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | the heterodimer E585A/wild-type Ncd construct exhibits 16% of the wild-type activity | Drosophila melanogaster | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.6.1.4 | heterodimer | although the heterodimers have one fully active and one inactive head, the ATPase and motility of Ncd heterodimers vary dramatically, clearly demonstrating that interactions between motor domains exist in Ncd | Drosophila melanogaster |
5.6.1.4 | homodimer | mutated homodimers have no microtubule-activated ATPase and no motility, whereas NcN have motility comparable with that of the wild-type Ncd | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.1.4 | kinesin-14 Ncd | Ncd, non-claret disjunctional is an often studied minus-end-directed motor in Drosophila melanogaster | Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.1.4 | 25 | - |
assay at | Drosophila melanogaster |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.1.4 | 0.1 | - |
ATP | mutant NcN-E585D, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.19 | - |
ATP | E585D/E585E, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.2 | - |
ATP | mutant E585A/wild-type, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.6 | - |
ATP | mutant E552A plus wild-type mix, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.6 | - |
ATP | mutant E552A/wild-type, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.6 | - |
ATP | mutant N600K plus wild-type mix, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.6 | - |
ATP | mutant NcN-E585D plus wild-type mix, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.7 | - |
ATP | mutant E585A plus wild-type mix, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.7 | - |
ATP | mutant E585D plus wild-type mix, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 0.9 | - |
ATP | E585D/wild-type, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 1.3 | - |
ATP | wild-type/wild-type, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 1.4 | - |
ATP | mutant N600K/wild-type, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster | |
5.6.1.4 | 1.9 | - |
ATP | mutant NcN, Ncd construct, ATPase activity of Ncd heterodimers and equimolar mixtures of homodimers at 5 microM microtubules, pH 7.2, 25°C | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.1.4 | 7.2 | - |
assay at | Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.6.1.4 | physiological function | the non-processive minus-end-directed kinesin-14 Ncd is involved in the organization of the microtubule network during mitosis. Only one of the two domains is involved in the interaction with the microtubules | Drosophila melanogaster |