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Literature summary extracted from

  • Kinne, M.; Poraj-Kobielska, M.; Ralph, S.A.; Ullrich, R.; Hofrichter, M.; Hammel, K.E.
    Oxidative cleavage of diverse ethers by an extracellular fungal peroxygenase (2009), J. Biol. Chem., 284, 29343-29349.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 1.43
-
 methyl 3,4-dimethoxybenzyl ether in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita
1.11.2.1 1.99
-
 H2O2 in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita
1.11.2.1 2.1
-
 tetrahydrofuran in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.2.1 extracellular
-
 Cyclocybe aegerita
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.1 44000
-
-
 Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1
-
 Cyclocybe aegerita

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 117
-
 using 3,4-dimethoxybenzyl alcohol as substrate, pH 7.0, 23°C Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 1,4-dimethoxybenzene + H2O2
-
 Cyclocybe aegerita 4-methoxyphenol + formaldehyde + H2O
-
 ?
1.11.2.1 1,4-dioxane + H2O2
-
 Cyclocybe aegerita 2-(2-hydroxyethoxy)acetaldehyde + H2O
-
 ?
1.11.2.1 1-(methoxymethyl)-4-nitrobenzene + H2O2
-
 Cyclocybe aegerita 4-nitrobenzaldehyde + methanol + H2O
-
 ?
1.11.2.1 2-(propan-2-yloxy)propane + H2O2
-
 Cyclocybe aegerita propan-2-one + propan-2-ol + H2O
-
 ?
1.11.2.1 4-nitroanisole + H2O2
-
 Cyclocybe aegerita formaldehyde + 4-nitrophenol + H2O
-
 ?
1.11.2.1 diethyl ether + H2O2
-
 Cyclocybe aegerita acetaldehyde + ethanol + H2O
-
 ?
1.11.2.1 methyl 3,4-dimethoxybenzyl ether + H2O2
-
 Cyclocybe aegerita 3,4-dimethoxybenzaldehyde + methanol + H2O
-
 ?
1.11.2.1 methyl 4-nitrobenzyl ether + H2O2
-
 Cyclocybe aegerita 4-nitrobenzaldehyde + methanol + H2O
-
 ?
1.11.2.1 methyl tert-butyl ether + H2O2
-
 Cyclocybe aegerita formaldehyde + tert-butanol + H2O
-
 ?
1.11.2.1 additional information the enzyme fails to cleave a 4-nitrophenyl-terminated polyethylene glycol Cyclocybe aegerita ?
-
 ?
1.11.2.1 tetrahydro-2H-pyran + H2O2
-
 Cyclocybe aegerita 5-hydroxypentanal + H2O
-
 ?
1.11.2.1 tetrahydrofuran + H2O2
-
 Cyclocybe aegerita 4-hydroxybutanal + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 33
-
 tetrahydrofuran in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita
1.11.2.1 720
-
 methyl 3,4-dimethoxybenzyl ether in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 7
-
 for the reaction with methyl 3,4-dimethoxybenzyl ether Cyclocybe aegerita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.2.1 5.4 8.4 for the reaction with methyl 3,4-dimethoxybenzyl ether Cyclocybe aegerita

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 500
-
 methyl 3,4-dimethoxybenzyl ether in potassium phosphate buffer (50 mM, pH 7.0), at 23°C Cyclocybe aegerita