BRENDA - Enzyme Database

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates

Takahashi, S.; Yeo, Y.S.; Zhao, Y.; O'Maille, P.E.; Greenhagen, B.T.; Noel, J.P.; Coates, R.M.; Chappell, J.; J. Biol. Chem. 282, 31744-31754 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.14.151
expressed in WAT11 yeast strain
Hyoscyamus muticus
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.14.151
A484I
the mutation results in a catalytically compromised enzyme
Hyoscyamus muticus
1.14.14.151
V366S
the mutation dramatically reduces overall enzyme activity
Hyoscyamus muticus
1.14.14.151
V480I/A484I
mutant with improved catalytic efficiency
Hyoscyamus muticus
1.14.14.151
V480S
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
1.14.14.151
V482I
mutant with improved catalytic efficiency
Hyoscyamus muticus
1.14.14.151
V482I
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
1.14.14.151
V482I/A484I
the mutant possesses a 5fold improvement in its catalytic efficiency for nootkatol biosynthesis and a 10fold improvement for 2beta-hydroxy-epiaristolochene biosynthesis
Hyoscyamus muticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.151
0.0025
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0033
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0043
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0062
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0069
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0074
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0084
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0121
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.013
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.014
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0179
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0192
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.151
microsome
-
Hyoscyamus muticus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.151
additional information
Hyoscyamus muticus
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.151
Hyoscyamus muticus
A6YIH8
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.151
(-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O
solavetivol is also named premnaspirodien-2alpha-ol or (2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol
-
-
?
1.14.14.151
4-epi-eremophilene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
4-epieremophilen-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.151
5-epiaristolochene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
substrate affinity for 5-epiaristolochene is 2-4times lower than that for valencene or (-)-premnaspirodiene, respectively. HPO catalyzes the conversion of 5-epiaristolochene to four mono-hydroxylated products with 2beta-hydroxy-epiaristolochene comprising greater than 80% of the reaction products, 1beta-hydroxy-epiaristolochene about 5%, 3alpha-hydroxy-epiaristolochene less than 2%, and an unknown mono-hydroxylated product of about 20%
712360
Hyoscyamus muticus
2beta-hydroxy-5-epiaristolochene + 1beta-hydroxy-5-epiaristolochene + 3alpha-hydroxy-5-epiaristolochene + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.14.151
cedr-8-ene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
cedr-8-en-15-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.151
additional information
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
712360
Hyoscyamus muticus
?
-
-
-
?
1.14.14.151
additional information
the enzyme fails to convert nootkatol to nootkatone
712360
Hyoscyamus muticus
?
-
-
-
?
1.14.14.151
valencene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
712360
Hyoscyamus muticus
alpha-nootkatol + beta-nootkatol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.14.151
HPO
-
Hyoscyamus muticus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.151
0.2
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.7
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1.4
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1.9
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
2.1
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
2.8
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
3.8
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
5.1
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
5.7
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
12.7
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
15.9
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
20.7
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.151
expressed in WAT11 yeast strain
Hyoscyamus muticus
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.14.151
A484I
the mutation results in a catalytically compromised enzyme
Hyoscyamus muticus
1.14.14.151
V366S
the mutation dramatically reduces overall enzyme activity
Hyoscyamus muticus
1.14.14.151
V480I/A484I
mutant with improved catalytic efficiency
Hyoscyamus muticus
1.14.14.151
V480S
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
1.14.14.151
V482I
mutant with improved catalytic efficiency
Hyoscyamus muticus
1.14.14.151
V482I
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
1.14.14.151
V482I/A484I
the mutant possesses a 5fold improvement in its catalytic efficiency for nootkatol biosynthesis and a 10fold improvement for 2beta-hydroxy-epiaristolochene biosynthesis
Hyoscyamus muticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.151
0.0025
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0033
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0043
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0062
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0069
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0074
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0084
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0121
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.013
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.014
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0179
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.0192
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.151
microsome
-
Hyoscyamus muticus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.151
additional information
Hyoscyamus muticus
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
?
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.151
(-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O
solavetivol is also named premnaspirodien-2alpha-ol or (2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol
-
-
?
1.14.14.151
4-epi-eremophilene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
4-epieremophilen-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.151
5-epiaristolochene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
substrate affinity for 5-epiaristolochene is 2-4times lower than that for valencene or (-)-premnaspirodiene, respectively. HPO catalyzes the conversion of 5-epiaristolochene to four mono-hydroxylated products with 2beta-hydroxy-epiaristolochene comprising greater than 80% of the reaction products, 1beta-hydroxy-epiaristolochene about 5%, 3alpha-hydroxy-epiaristolochene less than 2%, and an unknown mono-hydroxylated product of about 20%
712360
Hyoscyamus muticus
2beta-hydroxy-5-epiaristolochene + 1beta-hydroxy-5-epiaristolochene + 3alpha-hydroxy-5-epiaristolochene + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
1.14.14.151
cedr-8-ene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
cedr-8-en-15-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.151
additional information
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
712360
Hyoscyamus muticus
?
-
-
-
?
1.14.14.151
additional information
the enzyme fails to convert nootkatol to nootkatone
712360
Hyoscyamus muticus
?
-
-
-
?
1.14.14.151
valencene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
712360
Hyoscyamus muticus
alpha-nootkatol + beta-nootkatol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.151
0.2
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
0.7
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1.4
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1.9
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
2.1
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
2.8
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
3.8
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
5.1
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
5.7
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
12.7
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
15.9
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
20.7
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.14.151
60
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
80
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
150
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
260
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
280
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
300
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
610
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
650
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
740
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1310
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1510
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1590
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.14.151
60
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
80
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
150
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
260
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
280
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
300
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
610
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
650
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
740
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1310
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1510
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus
1.14.14.151
1590
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
Hyoscyamus muticus