Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, C.W.; Liao, J.C.
    Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution (2001), J. Biol. Chem., 276, 41161-41164.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.6 E508G random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
1.3.5.6 F166I random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene Cereibacter sphaeroides
1.3.5.6 F220S random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
1.3.5.6 H12Q random mutagenesis, the mutation has little effect on the enzyme activity Cereibacter sphaeroides
1.3.5.6 L148H random mutagenesis, the beneficial mutation is highly increasing the production of lycopene to over 80% Cereibacter sphaeroides
1.3.5.6 M402T random mutagenesis, the mutation reduces the lycopene production rate Cereibacter sphaeroides
1.3.5.6 additional information alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution combining random mutagenesis and site-directed mutagenesis, DNA sequence determinations. Beneficial mutations increase the percent of lycopene production to 90% Cereibacter sphaeroides
1.3.5.6 additional information introduction of stop mutations, M1, M2 and M3 Cereibacter sphaeroides
1.3.5.6 V68D random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene Cereibacter sphaeroides
1.3.99.28 F166I mutation changes the product of phytoene desaturation from neurosporene to lycopene Cereibacter sphaeroides
1.3.99.28 H12Q mutation has little effect on the product formation Cereibacter sphaeroides
1.3.99.28 M402T mutation has a negative effect on percent lycopene production Cereibacter sphaeroides
1.3.99.28 additional information directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change Cereibacter sphaeroides
1.3.99.28 V68D mutation changes the product of phytoene desaturation from neurosporene to lycopene Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.6 phytoene + quinone Cereibacter sphaeroides via phytofluene and zeta-carotene 7,9,9'-tricis-neurosporene + quinol
-
 ?
1.3.99.28 15-cis-phytoene + 3 acceptor Cereibacter sphaeroides the enzyme is involved in carotenoid biosynthesis all-trans-neurosporene + 3 reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.6 Cereibacter sphaeroides
-
 gene crtI
-
1.3.99.28 Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.6 7,9,9'-tricis-neurosporene + a quinone mutant activity Cereibacter sphaeroides 7,9,7',9'-tetracis-lycopene + a quinol
-
 ?
1.3.5.6 phytoene + quinone via phytofluene and zeta-carotene Cereibacter sphaeroides 7,9,9'-tricis-neurosporene + quinol
-
 ?
1.3.99.28 15-cis-phytoene + 3 acceptor the enzyme is involved in carotenoid biosynthesis Cereibacter sphaeroides all-trans-neurosporene + 3 reduced acceptor
-
 ?
1.3.99.28 phytoene + acceptor
-
 Cereibacter sphaeroides neurosporene + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.5.6 phytoene desaturase
-
 Cereibacter sphaeroides
1.3.99.28 CrtI
-
 Cereibacter sphaeroides

General Information

EC Number General Information Comment Organism
1.3.5.6 evolution approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene. These two types of enzymes, although similar in function, have relatively low similarity, below 60%, in terms of nucleotide or amino acid sequence. Phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme Cereibacter sphaeroides
1.3.5.6 physiological function phytoene desaturase encoded by crtI catalyzes the desaturation of phytoene in the carotenoid biosynthesis pathway, approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene, mechanism controlling the product specificity, phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme, overview Cereibacter sphaeroides
1.3.99.28 physiological function involved in carotenoid biosynthesis Cereibacter sphaeroides