EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.6 | E508G | random mutagenesis, the mutation has little effect on the enzyme activity | Cereibacter sphaeroides |
1.3.5.6 | F166I | random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene | Cereibacter sphaeroides |
1.3.5.6 | F220S | random mutagenesis, the mutation has little effect on the enzyme activity | Cereibacter sphaeroides |
1.3.5.6 | H12Q | random mutagenesis, the mutation has little effect on the enzyme activity | Cereibacter sphaeroides |
1.3.5.6 | L148H | random mutagenesis, the beneficial mutation is highly increasing the production of lycopene to over 80% | Cereibacter sphaeroides |
1.3.5.6 | M402T | random mutagenesis, the mutation reduces the lycopene production rate | Cereibacter sphaeroides |
1.3.5.6 | additional information | alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution combining random mutagenesis and site-directed mutagenesis, DNA sequence determinations. Beneficial mutations increase the percent of lycopene production to 90% | Cereibacter sphaeroides |
1.3.5.6 | additional information | introduction of stop mutations, M1, M2 and M3 | Cereibacter sphaeroides |
1.3.5.6 | V68D | random mutagenesis, a beneficial mutation that helps the conversion of product from neurosporene to lycopene | Cereibacter sphaeroides |
1.3.99.28 | F166I | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
1.3.99.28 | H12Q | mutation has little effect on the product formation | Cereibacter sphaeroides |
1.3.99.28 | M402T | mutation has a negative effect on percent lycopene production | Cereibacter sphaeroides |
1.3.99.28 | additional information | directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change | Cereibacter sphaeroides |
1.3.99.28 | V68D | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.6 | phytoene + quinone | Cereibacter sphaeroides | via phytofluene and zeta-carotene | 7,9,9'-tricis-neurosporene + quinol | - |
? | |
1.3.99.28 | 15-cis-phytoene + 3 acceptor | Cereibacter sphaeroides | the enzyme is involved in carotenoid biosynthesis | all-trans-neurosporene + 3 reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.6 | Cereibacter sphaeroides | - |
gene crtI | - |
1.3.99.28 | Cereibacter sphaeroides | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.6 | 7,9,9'-tricis-neurosporene + a quinone | mutant activity | Cereibacter sphaeroides | 7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
1.3.5.6 | phytoene + quinone | via phytofluene and zeta-carotene | Cereibacter sphaeroides | 7,9,9'-tricis-neurosporene + quinol | - |
? | |
1.3.99.28 | 15-cis-phytoene + 3 acceptor | the enzyme is involved in carotenoid biosynthesis | Cereibacter sphaeroides | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
1.3.99.28 | phytoene + acceptor | - |
Cereibacter sphaeroides | neurosporene + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.6 | phytoene desaturase | - |
Cereibacter sphaeroides |
1.3.99.28 | CrtI | - |
Cereibacter sphaeroides |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.5.6 | evolution | approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene. These two types of enzymes, although similar in function, have relatively low similarity, below 60%, in terms of nucleotide or amino acid sequence. Phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme | Cereibacter sphaeroides |
1.3.5.6 | physiological function | phytoene desaturase encoded by crtI catalyzes the desaturation of phytoene in the carotenoid biosynthesis pathway, approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosporene, whereas the rest produce lycopene, mechanism controlling the product specificity, phytoene desaturase encoded by gene crtI is a neurosporene-producing enzyme, overview | Cereibacter sphaeroides |
1.3.99.28 | physiological function | involved in carotenoid biosynthesis | Cereibacter sphaeroides |