Any feedback?
Literature summary extracted from
- Production, characterization, cloning and sequence analysis of a monofunctional catalase from Serratia marcescens SYBC08 (2011), J. Basic Microbiol., 51, 205-214.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.11.1.6 | industry | hydrogen peroxide scavenging enzyme | Serratia marcescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.6 | 78 | - |
H2O2 | in 50 mM NaH2PO4-Na2HPO4 buffer (pH 7.0) at 20°C | Serratia marcescens |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 58000 | - |
4 * 58000, SDS-PAGE | Serratia marcescens |
| 1.11.1.6 | 230000 | - |
gel filtration | Serratia marcescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.6 | Serratia marcescens | D7RJ66 | - |
- |
| 1.11.1.6 | Serratia marcescens SYBC08 | D7RJ66 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.6 | ammonium sulfate precipitation, DEAE column chromatography, and Sephacryl Tm S-200 gel filtration | Serratia marcescens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 14490 | - |
crude extract, pH 7.0, at 20°C | Serratia marcescens |
| 1.11.1.6 | 199600 | - |
after 13.8fold purification, pH 7.0, at 20°C | Serratia marcescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.6 | H2O2 | - |
Serratia marcescens | O2 + H2O | - |
? | |
| 1.11.1.6 | H2O2 | - |
Serratia marcescens SYBC08 | O2 + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.6 | homotetramer | 4 * 58000, SDS-PAGE | Serratia marcescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 20 | - |
- |
Serratia marcescens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | - |
- |
the enzyme has 78% activity at 0°C | Serratia marcescens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 60 | 70 | at 60°C, the enzyme is stable for 240 min, at 65°C the enzyme retains more than 55% of its activity by incubating the enzyme at pH 7.0 after 225 min, at 70°C, the enzyme can retain 57% of its initial activity after incubating at pH 7.0 for 90 min | Serratia marcescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 7 | 9 | - |
Serratia marcescens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.6 | 5 | 11 | - |
Serratia marcescens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.6 | heme | - |
Serratia marcescens | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.11.1.6 | physiological function | catalase plays a significant role in preventing Serratia marcescens against cellular damage through hydrogen peroxide | Serratia marcescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
