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Literature summary extracted from

  • Winward, L.; Whitfield, W.G.; McLennan, A.G.; Safrany, S.T.
    Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? (2010), Int. J. Biochem. Cell Biol., 42, 1174-1181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.52 expressed in Escherichia coli M15 cells as a hexahistidine-tagged fusion protein Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.52 0.00007
-
 diphosphoinositol pentakisphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.004
-
 adenosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.011
-
 guanosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.018
-
 diadenosine 5',5'''-P1,P6-hexaphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.13
-
 5-phospho-D-ribosyl 1-diphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.52 cytoplasm Aps is predominantly cytoplasmic Drosophila melanogaster 5737
-
3.6.1.52 nucleus
-
 Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.52 Co2+ Co2+ supports less than 5% of the activity with Mg2+ Drosophila melanogaster
3.6.1.52 Mg2+ Mg2+ is the most effective stimulatory cation above 10 mM Drosophila melanogaster
3.6.1.52 Mn2+ Mn2+ is also effective, with an optimal concentration of 1 mM Drosophila melanogaster
3.6.1.52 additional information Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps Drosophila melanogaster

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.1.52 dithiothreitol in the absence of dithiothreitol a 5 min pretreatment at 36°C lowers enzyme activity by 65%, with temperatures above 56°C virtually abolishing all activity, whereas in the presence of dithiothreitol a 5 min treatment at temperatures up to 76°C is without effect Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.52 Drosophila melanogaster
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.6.1.52 heat lability is restored by H2O2 Drosophila melanogaster

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.52 Ni-NTA column chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.52 adult
-
 Drosophila melanogaster
-
3.6.1.52 embryo
-
 Drosophila melanogaster
-
3.6.1.52 larva
-
 Drosophila melanogaster
-
3.6.1.52 pupa
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.52 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Drosophila melanogaster 5-phospho-alpha-D-ribose 1-phosphate + phosphate
-
 ?
3.6.1.52 8-oxo-dATP + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 8-oxo-dGTP + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 adenosine tetraphosphate + H2O the substrate shows great hydrolysis by Aps Drosophila melanogaster ?
-
 ?
3.6.1.52 ADP-glucose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 ADP-ribose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 CTP + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 diadenosine 5',5'''-P1,P5-pentaphosphate + H2O
-
 Drosophila melanogaster ?
-
 ?
3.6.1.52 diadenosine 5',5'''-P1,P6-hexaphosphate + H2O the substrate shows great hydrolysis by Aps Drosophila melanogaster ?
-
 ?
3.6.1.52 diguanosine pentaphosphate + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 diphosphoinositol pentakisphosphate + H2O
-
 Drosophila melanogaster inositol hexakisphosphate + phosphate
-
 ?
3.6.1.52 diphosphoinositol polyphosphate + H2O
-
 Drosophila melanogaster inositol polyphosphate + phosphate
-
 ?
3.6.1.52 dTTP + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 dUTP + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 GDP-glucose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 GDP-mannose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 guanosine tetraphosphate + H2O the substrate shows the greatest hydrolysis by Aps Drosophila melanogaster ?
-
 ?
3.6.1.52 additional information no hydrolysis of diadenosine 5',5'''-P1,P3-polyphosphate, diadenosine 5',5'''-P1,P2-polyphosphate, ATP, ADP, dATP, diguanosine tetraphosphate, diguanosine triphosphate, diguanosine diphosphate, GTP, GDP, dGTP, P1-(5'-adenosyl) P5-(5'-guanosyl) polyphosphate, and P1-(5'-adenosyl) P4-(5'-guanosyl) polyphosphate Drosophila melanogaster ?
-
 ?
3.6.1.52 TDP-glucose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 UDP-glucose + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 UDP-N-acetyl-D-galactosamine + H2O low activity Drosophila melanogaster ?
-
 ?
3.6.1.52 UDP-N-acetyl-D-glucosamine + H2O low activity Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.52 Aps protein the Nudix hydrolase Aps is a product of the sole Drosophila melanogaster DIPP/Aps1/Ddp1-like gene CG6391 Drosophila melanogaster
3.6.1.52 diphosphoinositol polyphosphate phosphohydrolase
-
 Drosophila melanogaster
3.6.1.52 DIPP
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.52 26
-
 at 10 mM Mg2+ Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.52 0.024
-
 diphosphoinositol pentakisphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.1
-
 5-phospho-D-ribosyl 1-diphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.24
-
 adenosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.34
-
 diadenosine 5',5'''-P1,P6-hexaphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 0.8
-
 guanosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.52 9
-
 at 10 mM Mg2+ Drosophila melanogaster

Expression

EC Number Organism Comment Expression
3.6.1.52 Drosophila melanogaster Aps expression is highest in embryos and declines throughout development down

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.52 0.8
-
 5-phospho-D-ribosyl 1-diphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 19
-
 diadenosine 5',5'''-P1,P6-hexaphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 60
-
 adenosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 71
-
 guanosine tetraphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster
3.6.1.52 342
-
 diphosphoinositol pentakisphosphate in the presence of 10 mM Mg2+, at pH 9.0 and 26°C Drosophila melanogaster