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Literature summary extracted from

  • Hausmann, A.; Sandmann, G.
    A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa (2000), Fungal Genet. Biol., 30, 147-153.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.99.30 NAD+ more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa Neurospora crassa
1.3.99.30 NADP+ stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.30 expression in Escherichia coli Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.30 62000
-
x * 62000, SDS-PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.30 15-cis-phytoene + 5 acceptor Neurospora crassa the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.30 Neurospora crassa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.99.30 15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor overall reaction Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.30 1-hydroxylycopene + acceptor
-
Neurospora crassa 1-hydroxy-3',4'-didehydrolycopene + reduced acceptor
-
?
1.3.99.30 1-hydroxyneurosporene + acceptor
-
Neurospora crassa 1-hydroxylycopene + reduced acceptor
-
?
1.3.99.30 15-cis-phytoene + 5 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?
1.3.99.30 15-cis-phytoene + 5 acceptor the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene Neurospora crassa all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?
1.3.99.30 all-trans-lycopene + acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + reduced acceptor
-
?
1.3.99.30 all-trans-neurosporene + 2 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
?
1.3.99.30 all-trans-phytofluene + 4 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
?
1.3.99.30 all-trans-zeta-carotene + 3 acceptor
-
Neurospora crassa all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
?
1.3.99.30 additional information gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate Neurospora crassa ?
-
?

Subunits

EC Number Subunits Comment Organism
1.3.99.30 ? x * 62000, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.3.99.30 Al-1
-
Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.30 30
-
assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.30 8
-
assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.30 additional information addition of FAD results in product formation close to that of the control without additions Neurospora crassa