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Literature summary extracted from

  • Okada, K.
    HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis (2009), FEBS Lett., 583, 1251-1256.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.5 PcyA expression in Escherichia coli strain BL21(DE3) Thermosynechococcus vestitus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.7.5 27000
-
 x * 27000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin Thermosynechococcus vestitus
-
 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.5 Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.5 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion-exchange chromatography, hydrophobic interaction chromatography, hydroxyapatite chromatography, and gel filtration Thermosynechococcus vestitus

Storage Stability

EC Number Storage Stability Organism
1.3.7.5 -30°C, purified recombinant enzyme, 50 mM HEPES-NaOH, pH 7.5, containing 100 mM NaCl, 6 months, full activity is maintained Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin
-
 Thermosynechococcus vestitus (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.7.5 ? x * 27000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus

Synonyms

EC Number Synonyms Comment Organism
1.3.7.5 Pcb:Fd oxidoreductase
-
 Thermosynechococcus vestitus
1.3.7.5 PcyA
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.7.5 25
-
 assay at Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.7.5 7.5
-
 assay at Thermosynechococcus vestitus

General Information

EC Number General Information Comment Organism
1.3.7.5 metabolism PcyA is involved in the phycocyanobilin biosynthetic pathway, in which PcyA first forms a stable complexes with the biliverdin IXa molecule Thermosynechococcus vestitus