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Literature summary extracted from

  • Ullrich, R.; Dolge, C.; Kluge, M.; Hofrichter, M.
    Pyridine as novel substrate for regioselective oxygenation with aromatic peroxygenase from Agrocybe aegerita (2008), FEBS Lett., 582, 4100-4106.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.069
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.2.1 extracellular
-
 Cyclocybe aegerita
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
 synonym Agrocybe cylindrical, strain A 1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 75
-
 isoform P II, using veratryl alcohol as substrate, pH 7.0, temperature not specified in the publication Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 2-chloropyridine + H2O2 26.1% conversion compared to pyridine Cyclocybe aegerita 2-chloropyridine N-oxide + H2O
-
 ?
1.11.2.1 3,5-dimethylpyridine + H2O2 143.4% conversion compared to pyridine Cyclocybe aegerita 5-methyl-nicotinic alcohol + 5-methyl-nicotinic aldehyde + 3,5-dimethylpyridine N-oxide + H2O 3,5-dimethylpyridine N-oxide is less than 1% of the converted substrate ?
1.11.2.1 3-bromopyridine + H2O2 61.8% conversion compared to pyridine Cyclocybe aegerita 3-bromopyridine N-oxide + H2O
-
 ?
1.11.2.1 3-chloropyridine + H2O2 47.2% conversion compared to pyridine Cyclocybe aegerita 3-chloropyridine N-oxide + nicotinic alcohol + nicotinic aldehyde + nicotinic acid + H2O
-
 ?
1.11.2.1 3-cyanopyridine + H2O2 moderate substrate with 25.4% conversion compared to pyridine Cyclocybe aegerita 3-cyanopyridine N-oxide + H2O
-
 ?
1.11.2.1 3-fluoropyridine + H2O2 39.4% conversion compared to pyridine Cyclocybe aegerita 3-fluoropyridine N-oxide + H2O
-
 ?
1.11.2.1 3-iodopyridine + H2O2 3-iodopyridine is slightly better oxidized than unsubstituted pyridine (102.2% conversion) Cyclocybe aegerita 3-iodopyridine N-oxide + H2O
-
 ?
1.11.2.1 3-methylpyridine + H2O2 98.4% conversion compared to pyridine Cyclocybe aegerita 3-methylpyridine N-oxide + H2O
-
 ?
1.11.2.1 3-nitropyridine + H2O2 moderate substrate with 5.4% conversion compared to pyridine Cyclocybe aegerita 3-nitropyridine N-oxide + H2O
-
 ?
1.11.2.1 4-chloropyridine + H2O2 4-chloropyridine is slightly better oxidized than unsubstituted pyridine (102.9% conversion) Cyclocybe aegerita 4-chloropyridine N-oxide + H2O
-
 ?
1.11.2.1 additional information nicotinic acid, nicotine amide, 3,5-dichloropyridine and perchloropyridine are no substrates Cyclocybe aegerita ?
-
 ?
1.11.2.1 pyridine + H2O2 100% conversion Cyclocybe aegerita pyridine N-oxide + H2O
-
 ?
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita
1.11.2.1 haloperoxidase
-
 Cyclocybe aegerita
1.11.2.1 haloperoxidase-peroxygenase
-
 Cyclocybe aegerita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 0.21
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 7
-
-
 Cyclocybe aegerita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.2.1 4 11
-
 Cyclocybe aegerita

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 3.04
-
 Pyridine in 10 mM phosphate buffer (pH 7.0), temperature not specified in the publication Cyclocybe aegerita