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Literature summary extracted from

  • Vrablik, T.L.; Huang, L.; Lange, S.E.; Hanna-Rose, W.
    Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival in C. elegans (2009), Development, 136, 3637-3646.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 isolation of a pnc-1a promoter consisting of exon 1a and the intervening region between exon 1a and the adjacent gene, expression as GFP-fusion protein. Expression of His-tagged PNC proteins in Escherichia coli strain BL21(DE3) Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 additional information mutations in PNC-1 cause developmental and functional defects in the reproductive system. The development of the gonad is delayed, four uterine cells die by necrosis and the mutant animals are egg-laying defective. The temporal delay in gonad development results from depletion of the salvage pathway product NAD+, whereas the uv1 cell necrosis and egg-laying defects result from accumulation of the substrate nicotinamide, phenotype, overview. The bacterial food source partially compensates for pnc-1 reproductive defects. Although the secreted isoform is sufficient to rescue all phenotypes, the concentrations of NAD+ and nicotinamide in the cytoplasm and/or cellular organelles presumably elicit phenotypes Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.19 extracellular secreted isoform of PNC-1 Caenorhabditis elegans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Caenorhabditis elegans
-
 nicotinate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Caenorhabditis elegans nicotinate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 PNC-1
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.19 30
-
 assay at Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 7.5
-
 assay at Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
3.5.1.19 metabolism nicotinamide phosphoribosyltransferase, Nampt, the equivalent enzyme in nicotinamide recycling to NAD+ in vertebrates, can functionally substitute for PNC-1 Caenorhabditis elegans
3.5.1.19 additional information a correlation between the limited detected expression patterns of the PNC-1a and b promoters and the function of PNC-1 in development is not obvious Caenorhabditis elegans