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Literature summary extracted from

  • Kim, S.G.; Jung, B.W.; Kim, H.
    Hemocyanin-derived phenoloxidase activity with broad temperature stability extending into the cold environment in hemocytes of the hair crab Erimacrus isenbeckii (2011), Comp. Biochem. Physiol. B, 159, 103-108.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 lipopolysaccharide phenoloxidase activity is highest at 0.2 mg/ml Erimacrus isenbeckii
1.10.3.1 lipoteichoic acid phenoloxidase activity is highest at 0.2 mg/ml Erimacrus isenbeckii
1.10.3.1 additional information beta-1,3-glucan has almost no effect on the phenoloxidase activity Erimacrus isenbeckii
1.10.3.1 SDS when 0.04 mM SDS is added, the phenoloxidase activity starts to increase and peaks at 5 mM SDS (125fold increase) Erimacrus isenbeckii
1.10.3.1 Trypsin when 0.001 mM trypsin is added, the phenoloxidase activity starts to increase and peaks at 0.04 mM trypsin (40fold increase) Erimacrus isenbeckii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 1-Phenyl-2-thiourea 94.4% inhibition at 0.0293 mM Erimacrus isenbeckii
1.10.3.1 EDTA 88.8% inhibition at 3.75 mM Erimacrus isenbeckii
1.10.3.1 N,N-diethyldithiocarbamate 94.4% inhibition at 0.0293 mM Erimacrus isenbeckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.96
-
 L-Dopa at 22°C, pH not specified in the publication Erimacrus isenbeckii
1.10.3.1 1.15
-
 catechol at 22°C, pH not specified in the publication Erimacrus isenbeckii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.1 Cu2+ copper-containing enzyme Erimacrus isenbeckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 75000
-
 6 * 75000, SDS-PAGE Erimacrus isenbeckii
1.10.3.1 448000
-
 gel filtration Erimacrus isenbeckii

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Erimacrus isenbeckii
-
 hair crab
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 DEAE-Sepharose column chromatography and Sephadex G-75 gel filtration Erimacrus isenbeckii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 hemocyte
-
 Erimacrus isenbeckii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2
-
 Erimacrus isenbeckii 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 L-dopa + O2
-
 Erimacrus isenbeckii dopachrome + H2O
-
 ?
1.10.3.1 additional information L-tyrosine and hydroquinone are ineffective as substrates Erimacrus isenbeckii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 homohexamer 6 * 75000, SDS-PAGE Erimacrus isenbeckii

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 phenoloxidase
-
 Erimacrus isenbeckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 4
-
-
 Erimacrus isenbeckii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 4 80 the phenoloxidase activity stays almost the same or decreases slightly up to 60°C, and decreases rapidly at 80°C. The average phenoloxidase activity increases at 4°C, 25°C, 40°C, and 60°C in a time-dependent manner, but no increase is observed at 80°C Erimacrus isenbeckii

General Information

EC Number General Information Comment Organism
1.10.3.1 physiological function cold-adapted hemocyanin-derived phenoloxidase activity is important to the survival of Erimacrus isenbeckii at low temperature (4°C) Erimacrus isenbeckii