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Literature summary extracted from

  • Harper, C.J.; Hayward, D.; Kidd, M.; Wiid, I.; van Helden, P.
    Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis (2010), BMC Microbiol., 10, 138.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.2 gene msmeg_4699, semi-quantitative real-time PCR expression analysis Mycolicibacterium smegmatis
1.4.1.4 gene msmeg_5442, semi-quantitative real-time PCR expression analysis Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 glycogen accumulation regulator GarA, native or unphosphorylated GarA is able to interact with NAD+-GDH causing a reduction in NAD+-GDH activity by altering the affinity of the enzyme for its substrate. This binding is prevented by the phosphorylation of GarA by PknG Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+ Mycolicibacterium smegmatis reaction cycle, specificities of forward and reverse reactions, overview 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.4 L-glutamate + H2O + NADP+ Mycolicibacterium smegmatis reaction cycle, specificities of forward and reverse reactions, overview 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Mycolicibacterium smegmatis A0R1C2 gene msmeg_4699
-
1.4.1.4 Mycolicibacterium smegmatis A0R3E3 gene msmeg_5442
-
6.3.1.2 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+ reaction cycle, specificities of forward and reverse reactions, overview Mycolicibacterium smegmatis 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.2 L-glutamate + H2O + NAD+ reaction cycle, overview Mycolicibacterium smegmatis 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.4 L-glutamate + H2O + NADP+ reaction cycle, specificities of forward and reverse reactions, overview Mycolicibacterium smegmatis 2-oxoglutarate + NH3 + NADPH + H+
-
 r
1.4.1.4 L-glutamate + H2O + NADP+ reaction cycle, overview Mycolicibacterium smegmatis 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 GDH2
-
 Mycolicibacterium smegmatis
1.4.1.2 More NAD+-specific glutamate dehydrogenases belongs to the L_180 class, that is affected by the binding of a small protein, GarA Mycolicibacterium smegmatis
1.4.1.2 NAD+-dependant glutamate dehydrogenase
-
 Mycolicibacterium smegmatis
1.4.1.2 NAD+-GDH
-
 Mycolicibacterium smegmatis
1.4.1.4 GDH1
-
 Mycolicibacterium smegmatis
1.4.1.4 NADP+ - dependant-glutamate dehydrogenase
-
 Mycolicibacterium smegmatis
1.4.1.4 NADP+-Gdh
-
 Mycolicibacterium smegmatis
6.3.1.2 GlnA1
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+ specific for Mycolicibacterium smegmatis
1.4.1.2 NADH specific for Mycolicibacterium smegmatis
1.4.1.4 NADP+ specific for Mycolicibacterium smegmatis
1.4.1.4 NADPH specific for Mycolicibacterium smegmatis

Expression

EC Number Organism Comment Expression
1.4.1.2 Mycolicibacterium smegmatis enzyme expression is upregulated under N2-starvation, overview up
1.4.1.4 Mycolicibacterium smegmatis enzyme expression is upregulated under N2-starvation, overview up
6.3.1.2 Mycolicibacterium smegmatis significant up-regulation of glnA1 gene transcription is observed within 0.5 hrs exposure to nitrogen starvation and continues to increase significantly thereafter up

General Information

EC Number General Information Comment Organism
1.4.1.2 metabolism NAD+-GDH plays an important role in nitrogen assimilation rather than glutamate catabolism, and is involved in the additional nitrogen assimilatory pathway via glutamate dehydrogenase, GDH. The specific activity of the deaminating NAD+-GDH reaction is mostly independent of nitrogen availability, overview, overview Mycolicibacterium smegmatis
1.4.1.4 metabolism NADP+-GDH is involved in nitrogen assimilation due to a constitutive aminating activity, specific activity of the aminating NADP+-GDH reaction is independent of nitrogen availability, it does not change significantly in response to prolonged exposure to nitrogen limitation, in contrast to the deaminating activity, which is 2fold increased exposed to ammonium starvation conditions. The deaminating reaction changes in response to varying ammonium concentrations and is regulated in response to nitrogen availability. NADP+-GDH is not regulated on the transcriptional level. The enzyme is invovled in the additional nitrogen assimilatory pathway via glutamate dehydrogenase, GDH, regulation of NADP+-GDH specific activity, overview Mycolicibacterium smegmatis
6.3.1.2 metabolism upon exposure to nitrogen limitation,GS activity increases significantly within 0.5 hrs and continues to increase significantly after 1 hr of nitrogen starvation to reach a final activity at 4 hrs that is approximately 2.5 fold greater than at zero hours. When an ammonium pulse is applied, GS activity decreases significantly within 1 hr of exposure to nitrogen excess Mycolicibacterium smegmatis