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Literature summary extracted from

  • Koreishi, M.; Nakatani, Y.; Ooi, M.; Imanaka, H.; Imamura, K.; Nakanishi, K.
    Purification, characterization, molecular cloning, and expression of a new aminoacylase from Streptomyces mobaraensis that can hydrolyze N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids (2009), Biosci. Biotechnol. Biochem., 73, 1940-1947.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.14 DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, expression in Streptomyces lividans strain TK24 resulting in 42fold higher activity compared to the native wild-type Streptomyces mobaraensis strain Streptomyces mobaraensis
3.5.1.14 expressed in Streptomyces lividans strain TK24 Streptomyces mobaraensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 1,10-phenanthroline 10% residual activity at 1 mM; 90% inhibition at 1 mM Streptomyces mobaraensis
3.5.1.14 2-mercaptoethanol 20% inhibition at 1 mM; 81% residual activity at 1 mM Streptomyces mobaraensis
3.5.1.14 Ca2+ 86% residual activity at 0.005 mM Streptomyces mobaraensis
3.5.1.14 Co2+ 82% residual activity at 0.005 mM; slight inhibition at 0.5 mM Streptomyces mobaraensis
3.5.1.14 Cu2+ 93% residual activity at 0.005 mM; slight inhibition at 0.5 mM Streptomyces mobaraensis
3.5.1.14 Fe2+ 86% residual activity at 0.005 mM; 90% inhibition at 0.5 mM Streptomyces mobaraensis
3.5.1.14 iodoacetamide 15% inhibition at 1 mM; 85% residual activity at 1 mM Streptomyces mobaraensis
3.5.1.14 Mg2+ 96% residual activity at 0.005 mM Streptomyces mobaraensis
3.5.1.14 Mn2+ 88% residual activity at 0.005 mM; slight inhibition at 0.5 mM Streptomyces mobaraensis
3.5.1.14 additional information the activity of the recombinant enzyme is not influenced by dithiothreitol, reduced glutathione, EDTA, p-chloromercuribenzoic acid, and L-Cys Streptomyces mobaraensis
3.5.1.14 Ni2+ slight inhibition at 0.5 mM Streptomyces mobaraensis
3.5.1.14 Zn2+ activates 57% at 500 nM, 20% at 0.005 mM, but inhibits 61% at 2.5 mM; slight inhibition at 0.5 mM; the relative remaining activity is decreased to 39% by incubation with 2.5 mM Zn2+ Streptomyces mobaraensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 11.3
-
 N-acetyl-L-Met recombinant enzyme, at 37°C, pH not specified in the publication Streptomyces mobaraensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 additional information the enzyme activity is not affected by Ca2+ or Mg2+ at 0.5 mM Streptomyces mobaraensis
3.5.1.14 Ni2+ 110% relative activity at 0.005 mM Streptomyces mobaraensis
3.5.1.14 Zn2+ 120% relative activity at 0.0005 mM Streptomyces mobaraensis
3.5.1.14 Zn2+ activates 57% at 500 nM, 20% at 0.005 mM, but inhibits 61% at 2.5 mM Streptomyces mobaraensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 50000
-
 SDS-PAGE Streptomyces mobaraensis
3.5.1.14 55000
-
-
 Streptomyces mobaraensis
3.5.1.14 55000
-
 blue native PAGE Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 additional information Streptomyces mobaraensis the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc ?
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O Streptomyces mobaraensis
-
 acetate + L-alanine
-
 ?
3.5.1.14 N-acetyl-L-cysteine + H2O Streptomyces mobaraensis
-
 acetate + L-cysteine
-
 ?
3.5.1.14 N-acetyl-L-methionine + H2O Streptomyces mobaraensis
-
 acetate + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Streptomyces mobaraensis C9K2Z6 strain NBRC (IFO) 13819
-
3.5.1.14 Streptomyces mobaraensis C9K2Z6 strain NBRC 13819
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 ammonium sulfate precipitation, octyl-Sepharose column chromatography, HiTrap chelating column chromatography, and DEAE Sephadex A-50 ion exchange chromatography Streptomyces mobaraensis
3.5.1.14 Streptomyces lividans strain TK24, native enzyme 494fold from by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography Streptomyces mobaraensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.14 additional information
-
 substrate specificities of recombinant and native enzyme, overview Streptomyces mobaraensis
3.5.1.14 0.89
-
 native wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 0.89
-
 recombinant wild-type enzyme, culture supernatant, substrate N-acetyl-L-methionine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 4
-
 using N-lauroyl-L-Arg as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 6
-
 using N-lauroyl-L-Asn as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 6
-
 using N-lauroyl-L-His as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 8
-
 using N-acetyl-Gly as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 8
-
 using N-acetyl-L-Asp as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 14
-
 using N-acetyl-L-Gln as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 14
-
 using N-lauroyl-L-Lys as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 17
-
 using N-lauroyl-L-Cys as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 19
-
 using N-acetyl-L-Phe as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 24
-
 using N-acetyl-L-Val as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 31
-
 using N-acetyl-L-Asn as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 34
-
 using N-lauroyl-Gly as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 36
-
 using N-lauroyl-L-Ser as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 59
-
 using N-acetyl-L-His as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 60
-
 using N-acetyl-L-Leu as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 66
-
 using N-acetyl-L-Arg as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 72
-
 using 3 mM N-lauroyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 89
-
 using 3 mM N-decanoyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 96
-
 purified recombinant wild-type enzyme, substrate N-lauryl-L-alanine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 96
-
 using N-lauroyl-L-Ala as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 105
-
 using 3 mM N-palmitoyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 106
-
 using 3 mM N-myristoyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 169
-
 using 3 mM N-butyryl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 173
-
 using 3 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 175
-
 using 3 mM N-hexanoyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 194
-
 using N-acetyl-L-Ala as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 251
-
 using 3 mM N-octanoyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 412
-
 purified recombinant wild-type enzyme, substrate N-acetyl-L-cysteine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 412
-
 using N-acetyl-L-Cys as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis
3.5.1.14 440
-
 purified native wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 530
-
 purified recombinant wild-type enzyme, substrate N-acetyl-L-methionine, pH 7.5, 37°C Streptomyces mobaraensis
3.5.1.14 530
-
 using 15 mM N-acetyl-L-Met as substrate, at 37°C, pH not specified in the publication Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 additional information the enzyme shows high hydrolytic activity towards various N-acetyl-L-amino acids and N-(middle/long)-chain-fatty-acyl-L-amino acids with a preference for the acyl derivatives of L-Met, L-Ala, L-Cys, etc Streptomyces mobaraensis ?
-
 ?
3.5.1.14 additional information no activity towards N-acetyl-L-Lys, N-acetyl-L-Glu, N-acetyl-L-Pro, N-acetyl-L-Trp, N-acetyl-L-Tyr, N-lauroyl-L-Ile, N-lauroyl-L-Leu, N-lauroyl-L-Phe, N-lauroyl-L-Pro, N-lauroyl-L-Trp, N-lauroyl-L-Tyr, and N-lauroyl-L-Val Streptomyces mobaraensis ?
-
 ?
3.5.1.14 additional information the enzyme hydrolyzes N-(middle/long)-chain-fatty-acyl-L-amino acids as well as N-short-chain-acyl-L-amino acids. Poor or no activity with N-acetyl-amino acids containing L-Lys, L-Asp, L-Cys, L-Pro, L-Trp, and L-Tyr, as well as with N-leuroyl-amino acids containing L-Arg, L-His, L-Asn, L-Ile, L-Leu, L-Phe, L-Pro, L-Trp, L-Tyr, and L-Val, substrate specificity, overview Streptomyces mobaraensis ?
-
 ?
3.5.1.14 N-Acetyl-Gly + H2O
-
 Streptomyces mobaraensis acetate + Gly
-
 ?
3.5.1.14 N-acetyl-L-Ala + H2O
-
 Streptomyces mobaraensis acetate + L-Ala
-
 ?
3.5.1.14 N-acetyl-L-alanine + H2O
-
 Streptomyces mobaraensis acetate + L-alanine
-
 ?
3.5.1.14 N-acetyl-L-Arg + H2O
-
 Streptomyces mobaraensis acetate + L-Arg
-
 ?
3.5.1.14 N-acetyl-L-arginine + H2O
-
 Streptomyces mobaraensis acetate + L-arginine
-
 ?
3.5.1.14 N-acetyl-L-Asn + H2O
-
 Streptomyces mobaraensis acetate + L-Asn
-
 ?
3.5.1.14 N-acetyl-L-Asp + H2O
-
 Streptomyces mobaraensis acetate + L-Asp
-
 ?
3.5.1.14 N-acetyl-L-asparagine + H2O
-
 Streptomyces mobaraensis acetate + L-asparagine
-
 ?
3.5.1.14 N-acetyl-L-Cys + H2O
-
 Streptomyces mobaraensis acetate + L-Cys
-
 ?
3.5.1.14 N-acetyl-L-cysteine + H2O
-
 Streptomyces mobaraensis acetate + L-cysteine
-
 ?
3.5.1.14 N-acetyl-L-Gln + H2O
-
 Streptomyces mobaraensis acetate + L-Gln
-
 ?
3.5.1.14 N-acetyl-L-glutamine + H2O
-
 Streptomyces mobaraensis acetate + L-glutamine
-
 ?
3.5.1.14 N-acetyl-L-histidine + H2O
-
 Streptomyces mobaraensis acetate + L-histidine
-
 ?
3.5.1.14 N-acetyl-L-Leu + H2O
-
 Streptomyces mobaraensis acetate + L-Leu
-
 ?
3.5.1.14 N-acetyl-L-leucine + H2O
-
 Streptomyces mobaraensis acetate + L-leucine
-
 ?
3.5.1.14 N-acetyl-L-Met + H2O highest activity Streptomyces mobaraensis acetate + L-Met
-
 ?
3.5.1.14 N-acetyl-L-methionine + H2O
-
 Streptomyces mobaraensis acetate + L-methionine
-
 ?
3.5.1.14 N-acetyl-L-methionine + H2O preferred substrate Streptomyces mobaraensis acetate + L-methionine
-
 ?
3.5.1.14 N-acetyl-L-Phe + H2O
-
 Streptomyces mobaraensis acetate + L-Phe
-
 ?
3.5.1.14 N-acetyl-L-phenylalanine + H2O
-
 Streptomyces mobaraensis acetate + L-phenylalanine
-
 ?
3.5.1.14 N-acetyl-L-Val + H2O
-
 Streptomyces mobaraensis acetate + L-Val
-
 ?
3.5.1.14 N-acetyl-L-valine + H2O
-
 Streptomyces mobaraensis acetate + L-valine
-
 ?
3.5.1.14 N-butyryl-L-Met + H2O
-
 Streptomyces mobaraensis butanoate + L-Met
-
 ?
3.5.1.14 N-butyryl-L-methionine + H2O
-
 Streptomyces mobaraensis butyrate + L-methionine
-
 ?
3.5.1.14 N-decanoyl-L-Met + H2O
-
 Streptomyces mobaraensis decanoate + L-Met
-
 ?
3.5.1.14 N-decanoyl-L-methionine + H2O
-
 Streptomyces mobaraensis decanoate + L-methionine
-
 ?
3.5.1.14 N-hexanoyl-L-Met + H2O
-
 Streptomyces mobaraensis hexanoate + L-Met
-
 ?
3.5.1.14 N-hexanoyl-L-methionine + H2O
-
 Streptomyces mobaraensis hexanoate + L-methionine
-
 ?
3.5.1.14 N-lauroyl-Gly + H2O
-
 Streptomyces mobaraensis laurate + Gly
-
 ?
3.5.1.14 N-lauroyl-L-Ala + H2O
-
 Streptomyces mobaraensis laurate + L-Ala
-
 ?
3.5.1.14 N-lauroyl-L-Arg + H2O
-
 Streptomyces mobaraensis laurate + L-Arg
-
 ?
3.5.1.14 N-lauroyl-L-Asn + H2O
-
 Streptomyces mobaraensis laurate + L-Asn
-
 ?
3.5.1.14 N-lauroyl-L-Cys + H2O
-
 Streptomyces mobaraensis laurate + L-Cys
-
 ?
3.5.1.14 N-lauroyl-L-His + H2O
-
 Streptomyces mobaraensis laurate + L-His
-
 ?
3.5.1.14 N-lauroyl-L-Lys + H2O
-
 Streptomyces mobaraensis laurate + L-Lys
-
 ?
3.5.1.14 N-lauroyl-L-Met + H2O
-
 Streptomyces mobaraensis laurate + L-Met
-
 ?
3.5.1.14 N-lauroyl-L-Ser + H2O
-
 Streptomyces mobaraensis laurate + L-Ser
-
 ?
3.5.1.14 N-lauryl-glycine + H2O
-
 Streptomyces mobaraensis laurate + glycine
-
 ?
3.5.1.14 N-lauryl-L-alanine + H2O
-
 Streptomyces mobaraensis laurate + L-alanine
-
 ?
3.5.1.14 N-lauryl-L-cysteine + H2O
-
 Streptomyces mobaraensis laurate + L-cysteine
-
 ?
3.5.1.14 N-lauryl-L-lysine + H2O
-
 Streptomyces mobaraensis laurate + L-lysine
-
 ?
3.5.1.14 N-lauryl-L-methionine + H2O
-
 Streptomyces mobaraensis laurate + L-methionine
-
 ?
3.5.1.14 N-lauryl-L-serine + H2O
-
 Streptomyces mobaraensis laurate + L-serine
-
 ?
3.5.1.14 N-myristoyl-L-Met + H2O
-
 Streptomyces mobaraensis myristate + L-Met
-
 ?
3.5.1.14 N-myristoyl-L-methionine + H2O
-
 Streptomyces mobaraensis myristate + L-methionine
-
 ?
3.5.1.14 N-octanoyl-L-Met + H2O
-
 Streptomyces mobaraensis octanoate + L-Met
-
 ?
3.5.1.14 N-octanoyl-L-methionine + H2O
-
 Streptomyces mobaraensis octanoate + L-methionine
-
 ?
3.5.1.14 N-palmitoyl-L-Met + H2O
-
 Streptomyces mobaraensis palmitate + L-Met
-
 ?
3.5.1.14 N-palmitoyl-L-methionine + H2O
-
 Streptomyces mobaraensis palmitate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 monomer 1 * 55000 Streptomyces mobaraensis
3.5.1.14 monomer 1 * 55000, purified wild type enzyme, SDS-PAGE Streptomyces mobaraensis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 More the enzyme belongs to the peptidase M20 family Streptomyces mobaraensis
3.5.1.14 N-acyl-L-amino-acid amidohydrolase
-
 Streptomyces mobaraensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 50
-
-
 Streptomyces mobaraensis
3.5.1.14 50
-
 at pH 7.5 Streptomyces mobaraensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 7.5
-
-
 Streptomyces mobaraensis