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Literature summary extracted from

  • Wu, Y.R.; Luo, Z.H.; Kwok-Kei Chow, R.; Vrijmoed, L.L.
    Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2 (2010), Biores. Technol., 101, 9772-9777.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 SDS 131.1% relative activity in the presence of 2 mM SDS Fusarium solani

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Ag+ complete inhibition at 20 mM Fusarium solani
1.10.3.2 Cd2+ 71.1% residual activity at 20 mM Fusarium solani
1.10.3.2 Co2+ 61.9% residual activity at 20 mM Fusarium solani
1.10.3.2 Cr3+ 46.2% residual activity at 20 mM Fusarium solani
1.10.3.2 Cu2+ 68.9% residual activity at 20 mM Fusarium solani
1.10.3.2 dithiothreitol complete inhibition at 2 mM Fusarium solani
1.10.3.2 EDTA 20.8% residual activity at 20 mM Fusarium solani
1.10.3.2 Fe2+ 4.0% residual activity at 20 mM Fusarium solani
1.10.3.2 Fe3+ complete inhibition at 20 mM Fusarium solani
1.10.3.2 Mn2+ 49.1% residual activity at 20 mM Fusarium solani
1.10.3.2 NaN3 complete inhibition at 2 mM Fusarium solani
1.10.3.2 Ni2+ 62% residual activity at 20 mM Fusarium solani
1.10.3.2 Pb2+ 97.2% residual activity at 20 mM Fusarium solani
1.10.3.2 SDS 93.8% residual activity at 20 mM Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0086
-
 2,6-dimethoxyphenol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 0.0794
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 2.657
-
 guaiacol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 3.434
-
 catechol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Fusarium solani
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Hg2+ 130.7% relative activity in the presence of 20 mM Hg2+ Fusarium solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 72000
-
 SDS–PAGE Fusarium solani

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 Acetone the laccase retains about 60% of its activity in the presence of 5% (v/v) acetone Fusarium solani
1.10.3.2 acetonitrile the laccase retains about 75% of its activity in the presence of 5% (v/v) acetonitrile Fusarium solani
1.10.3.2 DMSO the laccase retains about 70% of its activity in the presence of 5% (v/v) DMSO Fusarium solani
1.10.3.2 Ethanol the laccase retains about 75% of its activity in the presence of 5% (v/v) ethanol Fusarium solani
1.10.3.2 Methanol the laccase retains more than 75-80% of its activity in the presence of 5% (v/v) methanol Fusarium solani

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Fusarium solani
-
-
-
1.10.3.2 Fusarium solani MAS2
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein
-
 Fusarium solani

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 HiTrap DEAE column chromatography and Sephacryl S-200 gel filtration Fusarium solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 5.19
-
 crude culture, at 30°C, pH 4.5 Fusarium solani
1.10.3.2 358.7
-
 after 69.1fold purification, at 30°C, pH 4.5 Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Fusarium solani ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Fusarium solani MAS2 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 best substrate Fusarium solani ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 best substrate Fusarium solani MAS2 ?
-
 ?
1.10.3.2 catechol + O2
-
 Fusarium solani ?
-
 ?
1.10.3.2 catechol + O2
-
 Fusarium solani MAS2 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Fusarium solani ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Fusarium solani MAS2 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Fusarium solani ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Fusarium solani MAS2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 72000, native PAGE Fusarium solani

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 benzenediol: oxygen oxidoreductase
-
 Fusarium solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
-
 Fusarium solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50 70 the enzyme retains activity after incubation at 50°C for 1 h, but the activity sharply decreases when the temperature is increased to 60°C and almost no activity is detected at 70°C. The laccase can still oxidize 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) when incubated at 70°C for 5 min, but when the enzyme is incubated under the same condition without substrate, it loses almost all its activity Fusarium solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 28.4
-
 guaiacol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 44.9
-
 catechol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 107.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 121
-
 2,6-dimethoxyphenol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
-
 Fusarium solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 12 the purified laccase maintains stable at pH 3.0-11.0. The enzyme remains active at pH values from 3.0 to 12.0, and its activity at pH 2.0 and 12.0 are 56.6% and 85.5% of that at pH 7.0, respectively Fusarium solani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 11
-
 guaiacol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 13
-
 catechol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 1300
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani
1.10.3.2 14000
-
 2,6-dimethoxyphenol at 30°C in 100 mM sodium tartrate buffer (pH 4.5) Fusarium solani