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Literature summary extracted from

  • Kumar, S.; Mohan, U.; Kamble, A.L.; Pawar, S.; Banerjee, U.C.
    Cross-linked enzyme aggregates of recombinant Pseudomonas putida nitrilase for enantioselective nitrile hydrolysis (2010), Biores. Technol., 101, 6856-6858.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

General Stability

EC Number General Stability Organism
3.5.5.1 nitrilase aggregates are prepared using ammonium sulfate (35%) precipitation followed by cross-linking with glutaraldehyde (125 mM) which renders 70% activity retention Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Pseudomonas putida
-
-
-
3.5.5.1 Pseudomonas putida MTCC 5110
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 35% (w/v) ammonium sulfate precipitation Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 mandelonitrile + 2 H2O
-
 Pseudomonas putida mandelic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O
-
 Pseudomonas putida MTCC 5110 mandelic acid + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 4
-
 activity of cross-linked enzyme aggregate preparation remains stable up to 75 h at 4°C while the free enzyme loses 90% activity at this temperature in 75 h Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7
-
 the activity in the CLEA increases with the increase in the pH of cross-linking solution and it is maximum at pH 7.0 Pseudomonas putida