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Literature summary extracted from

  • Kwon, Y.J.; Fang, Y.; Xu, G.H.; Kim, W.G.
    Aquastatin A, a new inhibitor of enoyl-acyl carrier protein reductase from Sporothrix sp. FN611 (2009), Biol. Pharm. Bull., 32, 2061-2064.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.9 drug development the bacterial enoyl-ACP reductase is a target for antibacterial drug development Streptococcus pneumoniae
1.3.1.10 drug development the bacterial enoyl-ACP reductase is a target for antibacterial drug development Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 aquastatin A a natural inhibitor from the fungus Sporothrix sp. strain FN611, prevents the growth of Staphylococcus pneumoniae with minimum inhibitory concentration of 0.064-0.128 mg/ml, overview Streptococcus pneumoniae
1.3.1.9 degalactosylated aquastatin A a natural inhibitor from the fungus Sporothrix sp. strain FN611 Streptococcus pneumoniae
1.3.1.10 aquastatin A a natural inhibitor from the fungus Sporothrix sp. strain FN611, mixed-type inhibition against FabI with respect to the substrate trans-2-octenoyl N-acetylcysteamine and NADPH, prevents the growth of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus with minimum inhibitory concentration of 0.016-0.032 mg/ml, overview Staphylococcus aureus
1.3.1.10 degalactosylated aquastatin A a natural inhibitor from the fungus Sporothrix sp. strain FN611 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Streptococcus pneumoniae
-
-
-
1.3.1.9 Streptococcus pneumoniae KCTC 3932
-
-
-
1.3.1.10 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 additional information activity measurement by reduction of the trans-2-octenoyl N-acetylcysteamine as substrate and NADH as cofactor Streptococcus pneumoniae ?
-
 ?
1.3.1.9 additional information activity measurement by reduction of the trans-2-octenoyl N-acetylcysteamine as substrate and NADH as cofactor Streptococcus pneumoniae KCTC 3932 ?
-
 ?
1.3.1.10 additional information activity measurement by reduction of the trans-2-octenoyl N-acetylcysteamine as substrate and NADPH as cofactor Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-ACP reductase
-
 Streptococcus pneumoniae
1.3.1.9 enoyl-acyl carrier protein reductase
-
 Streptococcus pneumoniae
1.3.1.9 FabK
-
 Streptococcus pneumoniae
1.3.1.10 enoyl-ACP reductase
-
 Staphylococcus aureus
1.3.1.10 enoyl-acyl carrier protein reductase
-
 Staphylococcus aureus
1.3.1.10 FabI
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.9 30
-
 assay at Streptococcus pneumoniae
1.3.1.10 30
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 6.5
-
 assay at Streptococcus pneumoniae
1.3.1.10 6.5
-
 assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Streptococcus pneumoniae
1.3.1.10 NADPH
-
 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.10 0.0014
-
 aquastatin A pH 6.5, 30°C, versus NADPH Staphylococcus aureus
1.3.1.10 0.00161
-
 aquastatin A pH 6.5, 30°C, versus trans-2-octenoyl N-acetylcysteamine Staphylococcus aureus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.9 0.0092
-
 FabK, pH 6.5, 30°C Streptococcus pneumoniae aquastatin A
1.3.1.9 0.0106
-
 FabI, pH 6.5, 30°C Streptococcus pneumoniae degalactosylated aquastatin A
1.3.1.10 0.0032
-
 FabI, pH 6.5, 30°C Staphylococcus aureus aquastatin A
1.3.1.10 0.0034
-
 FabI, pH 6.5, 30°C Staphylococcus aureus degalactosylated aquastatin A