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Literature summary extracted from

  • Wei, M.; Zhang, J.J.; Liu, H.; Zhou, N.Y.
    para-Nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in Pseudomonas sp. strain WBC-3 (2010), Biodegradation, 21, 915-921.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.6.5.6 environmental protection the strain WBC3, also possessing 4-nitrophenyl 4-monooxygenase activity through PnpA, has a potential in bioremediation of the environment polluted by both 4-nitrocatechol and 4-nitrophenol Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.37 expressed in Escherichia coli BL21 cells Pseudomonas sp.
1.14.13.166 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.
1.14.13.167 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.37 soluble
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 34800
-
 estimated from SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.6 additional information Pseudomonas sp. strain WBC-3 can completely degrade both 4-nitrophenol and 4-nictrocatechol when 4-nitrophenol is used as the inducer ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.6 Pseudomonas sp.
-
 gene pnpB
-
1.13.11.37 Pseudomonas sp.
-
-
-
1.14.13.166 Pseudomonas sp.
-
-
-
1.14.13.167 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.37 Ni-NTA column chromatography Pseudomonas sp.
1.14.13.166 Ni-NTA agarose column chromatography Pseudomonas sp.
1.14.13.167 Ni-NTA agarose column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.6 additional information strain WBC-3 can completely degrade both 4-nitrophenol and 4-nictrocatechol when 4-nitrophenol is used as the inducer Pseudomonas sp. ?
-
 ?
1.13.11.37 catechol + O2
-
 Pseudomonas sp. cis,cis-muconic acid
-
 ?
1.13.11.37 hydroxyquinol + O2 intradiol ringcleavage Pseudomonas sp. maleylacetate
-
 ?
1.13.11.37 additional information PnpG is not active against protocatechuate and gentisate Pseudomonas sp. ?
-
 ?
1.14.13.166 4-nitrocatechol + NADPH + H+ + O2 monooxygenation, the enzyme completely degrades 0.1 mM 4-nitrocatechol in 80 min Pseudomonas sp. 1,2,4-trihydroxybenzene + nitrite + NADP+ + H2O
-
 ?
1.14.13.167 4-nitrophenol + NADPH + H+ + O2 the enzyme completely degrades 0.24 mM 4-nitrophenol in 36 min Pseudomonas sp. 4-benzoquinone + nitrite + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.6 para-benzoquinone reductase
-
 Pseudomonas sp.
1.6.5.6 PnpB
-
 Pseudomonas sp.
1.13.11.37 PnpG
-
 Pseudomonas sp.
1.14.13.166 para-nitrophenol 4-monooxygenase
-
 Pseudomonas sp.
1.14.13.166 PNP 4-monooxygenase
-
 Pseudomonas sp.
1.14.13.166 pnpA
-
 Pseudomonas sp.
1.14.13.167 para-nitrophenol 4-monooxygenase
-
 Pseudomonas sp.
1.14.13.167 PNP 4-monooxygenase
-
 Pseudomonas sp.
1.14.13.167 pnpA
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.166 FAD
-
 Pseudomonas sp.
1.14.13.167 FAD
-
 Pseudomonas sp.

Expression

EC Number Organism Comment Expression
1.6.5.6 Pseudomonas sp. no induction by 4-nitrocatechol additional information
1.6.5.6 Pseudomonas sp. induction b para-nitrophenol up
1.14.13.166 Pseudomonas sp. enzyme expression can be induced by 4-nitrophenol but not 4-nitrocatechol up
1.14.13.167 Pseudomonas sp. enzyme expression can be induced by 4-nitrophenol but not 4-nitrocatechol up

General Information

EC Number General Information Comment Organism
1.6.5.6 metabolism the enzyme is involved in the initial steps of 4-nitrophenol catabolism via hydroquinone Pseudomonas sp.