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Literature summary extracted from

  • Quesada-Soriano, I.; Casas-Solvas, J.M.; Recio, E.; Ruiz-Perez, L.M.; Vargas-Berenguel, A.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
    Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPases (2010), Biochimie, 92, 178-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 dTMP
-
 Homo sapiens
3.6.1.23 dTMP
-
 Plasmodium falciparum
3.6.1.23 fluorodeoxy-UMP
-
 Homo sapiens
3.6.1.23 fluorodeoxy-UMP
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0011
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.00173
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.00187
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.0061
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.29
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.438
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.465
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.523
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.54
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.571
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.687
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.761
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 1.13
-
 dATP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 1.96
-
 dGTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Homo sapiens
-
-
-
3.6.1.23 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Homo sapiens
3.6.1.23
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 2'-deoxy-5-fluoro-UTP + H2O most specific substrate Homo sapiens 2'-deoxy-5-fluoro-UMP + diphosphate
-
 ?
3.6.1.23 2'-deoxy-5-fluoro-UTP + H2O most specific substrate Plasmodium falciparum 2'-deoxy-5-fluoro-UMP + diphosphate
-
 ?
3.6.1.23 beta-L-2'-dUTP + H2O
-
 Homo sapiens beta-L-2'-dUMP + diphosphate
-
 ?
3.6.1.23 beta-L-2'-dUTP + H2O
-
 Plasmodium falciparum beta-L-2'-dUMP + diphosphate
-
 ?
3.6.1.23 dATP + H2O
-
 Homo sapiens dAMP + diphosphate
-
 ?
3.6.1.23 dATP + H2O
-
 Plasmodium falciparum dAMP + diphosphate
-
 ?
3.6.1.23 dCTP + H2O
-
 Homo sapiens dCMP + diphosphate
-
 ?
3.6.1.23 dCTP + H2O
-
 Plasmodium falciparum dCMP + diphosphate
-
 ?
3.6.1.23 dGTP + H2O
-
 Homo sapiens dGMP + diphosphate
-
 ?
3.6.1.23 dGTP + H2O
-
 Plasmodium falciparum dGMP + diphosphate
-
 ?
3.6.1.23 dTTP + H2O
-
 Homo sapiens dTMP + diphosphate
-
 ?
3.6.1.23 dTTP + H2O
-
 Plasmodium falciparum dTMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O most specific substrate Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O most specific substrate Plasmodium falciparum dUMP + diphosphate
-
 ?
3.6.1.23 UTP + H2O
-
 Homo sapiens UMP + diphosphate
-
 ?
3.6.1.23 UTP + H2O
-
 Plasmodium falciparum UMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Homo sapiens
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Plasmodium falciparum
3.6.1.23 dUTPase
-
 Homo sapiens
3.6.1.23 dUTPase
-
 Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.23 0.00013
-
 dGTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.00265
-
 dATP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.00525
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.0061
-
 beta-L-2'-dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.0092
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.01121
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.01278
-
 UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.01971
-
 dTTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 0.02172
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 0.1616
-
 dCTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 110
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum
3.6.1.23 600
-
 2'-deoxy-5-fluoro-UTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 6600
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Homo sapiens
3.6.1.23 7100
-
 dUTP at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 Plasmodium falciparum