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Literature summary extracted from

  • Micalella, C.; Martignon, S.; Bruno, S.; Pioselli, B.; Caglio, R.; Valetti, F.; Pessione, E.; Giunta, C.; Rizzi, M.
    X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: A multidisciplinary characterization of catechol 1,2 dioxygenase (2011), Biochim. Biophys. Acta, 1814, 817-823.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expressed in Escherichia coli BL21(DE3) cells Acinetobacter radioresistens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.1 hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, at 4°C Acinetobacter radioresistens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.1 A72G the mutant shows decreased activity with catechol and no activity with 3-methylcatechol compared to the wild type enzyme Acinetobacter radioresistens
1.13.11.1 L69A the mutant shows decreased activity with catechol and increased activity for 4-chlorocatechol compared to the wild type enzyme Acinetobacter radioresistens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0001
-
4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.00198
-
4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.00204
-
catechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.00225
-
3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.00576
-
catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.00595
-
3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.0061
-
4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.0093
-
catechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.01
-
4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.0128
-
4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.053
-
4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Fe3+ Fe3+-dependent enzyme Acinetobacter radioresistens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter radioresistens Q9F103
-
-
1.13.11.1 Acinetobacter radioresistens LMG S13 Q9F103
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 Q-Sepharose column chromatography, gel filtration Acinetobacter radioresistens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
1.13.11.1 3-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4,5-dichlorocatechol + O2 the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol Acinetobacter radioresistens ?
-
?
1.13.11.1 4,5-dichlorocatechol + O2 the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol Acinetobacter radioresistens LMG S13 ?
-
?
1.13.11.1 4-chlorocatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
?
1.13.11.1 4-chlorocatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens 4-methyl-cis,cis-muconate
-
?
1.13.11.1 4-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 4-methyl-cis,cis-muconate
-
?
1.13.11.1 catechol + O2 best substrate Acinetobacter radioresistens cis,cis-muconate
-
?
1.13.11.1 catechol + O2 best substrate Acinetobacter radioresistens LMG S13 cis,cis-muconate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 1,2 CTD
-
Acinetobacter radioresistens
1.13.11.1 catechol 1,2 dioxygenase
-
Acinetobacter radioresistens
1.13.11.1 IsoB
-
Acinetobacter radioresistens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 0.17
-
4,5-dichlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.17
-
4,5-dichlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.18
-
4,5-dichlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.34
-
4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.52
-
3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.72
-
4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 0.83
-
4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 1.59
-
3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 1.74
-
4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 4.42
-
catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 5.45
-
4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 5.51
-
4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 13.6
-
catechol mutant enzyme A72G, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 24.2
-
catechol wild type enzyme, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.1 280
-
4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 550
-
4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 570
-
4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 880
-
3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 1000
-
4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 1460
-
catechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 3440
-
4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 4200
-
4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 7100
-
3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 7800
-
catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.13.11.1 11800
-
catechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens