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Literature summary extracted from

  • Witzke, A.; Lindner, K.; Munson, K.; Apell, H.J.
    Inhibition of the gastric H,K-ATPase by clotrimazole (2010), Biochemistry, 49, 4524-4532.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.19 clotrimazole molecular mechanism of the inhibitory effect is studied by steady-state fluorescence experiments with the electrochromic styryl dye RH421 Sus scrofa
7.2.2.19 K+ activates, required. But in the presence of 200m M KCl, ion binding sites are more than 99% saturated with K+. The enzyme activity under this condition is near zero Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.19 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.19 K+ activates, required. But in the presence of 200m M KCl, ion binding sites are more than 99% saturated with K+. The enzyme activity under this condition is near zero Sus scrofa
7.2.2.19 Mg2+ dependent on Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.19 ATP + H2O + H+/in + K+/out Sus scrofa
-
 ADP + phosphate + H+/out + K+/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.19 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.19 native gastric H,K-ATPase from gastric mucosa by a method involving differential and density gradient centrifugation, e.g. sucrose step gradient centrifugation, and plasma vesicle formation oriented as in the parietal cell with the cytoplasmic side outward Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.2.19 gastric mucosa
-
 Sus scrofa
-
7.2.2.19 stomach
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.19 ATP + H2O + H+/in + K+/out
-
 Sus scrofa ADP + phosphate + H+/out + K+/in
-
 ?
7.2.2.19 ATP + H2O + H+/in + K+/out The second ion species that can bind to the H,K-ATPase in the E1 conformation is K+ that competes with H+. Experimental activity measurement begins with the ion pump in the E1 state after treatment with nigericin Sus scrofa ADP + phosphate + H+/out + K+/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.19 gastric H,K-ATPase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.19 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.2.19 7.2
-
 assay at Sus scrofa