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Literature summary extracted from

  • Rodriguez-Acebes, S.; de la Cueva, P.; Fernandez-Hernando, C.; Ferruelo, A.J.; Lasuncion, M.A.; Rawson, R.B.; Martinez-Botas, J.; Gomez-Coronado, D.
    Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-DELTA24-reductase-deficient cell line (2009), Biochem. J., 420, 305-315.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.72 quantitative real-time PCR DHCR24 expression analysis Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.72 5alpha-cholest-7-en-3beta-ol + NADP+ Mus musculus
-
 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.72 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.72 additional information no activity in J774 cells, that lack a full-length transcript for DHCR24. J774 cells are able to grow indefinitely after the virtually total replacement of cholesterol by desmosterol, i.e. J774-D cells Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.72 5alpha-cholest-7-en-3beta-ol + NADP+
-
 Mus musculus 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.3.1.72 3beta -hydroxysterol DELTA24-reductase
-
 Mus musculus
1.3.1.72 DHCR24
-
 Mus musculus
1.3.1.72 sterol-DELTA24-reductase
-
 Mus musculus

General Information

EC Number General Information Comment Organism
1.3.1.72 metabolism desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, while 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, can do so, pathway regulation, detailed overview Mus musculus
1.3.1.72 additional information J774 cells, that lack a full-length transcript for DHCR24, consequently accumulate desmosterol Mus musculus
1.3.1.72 additional information while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required Mus musculus