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Literature summary extracted from

  • Kinne, M.; Zeisig, C.; Ullrich, R.; Kayser, G.; Hammel, K.E.; Hofrichter, M.
    Stepwise oxygenations of toluene and 4-nitrotoluene by a fungal peroxygenase (2010), Biochem. Biophys. Res. Commun., 397, 18-21.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.1 4-nitrotoluene the low extent of 4-nitrotoluene oxidation is attributable to inhibition of the enzyme by the substrate Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1
-
 Cyclocybe aegerita

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 117
-
 using 3,4-dimethoxybenzyl alcohol as substrate, at 23°C, pH not specified in the publication Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 3,4-dimethoxybenzyl alcohol + H2O2
-
 Cyclocybe aegerita 3,4-dimethoxybenzaldehyde + H2O
-
 ?
1.11.2.1 4-nitrobenzaldehyde + H2O2
-
 Cyclocybe aegerita 4-nitrobenzoic acid + H2O
-
 ?
1.11.2.1 4-nitrobenzyl alcohol + H2O2
-
 Cyclocybe aegerita 4-nitrobenzaldehyde + H2O
-
 ?
1.11.2.1 4-nitrotoluene + H2O2 APO hydroxylates 4-nitrotoluene to 4-nitrobenzyl alcohol, then to 4-nitrobenzaldehyde and then to 4-nitrobenzoic acid. The reactions proceed stepwise with total conversions of 12% for 4-nitrotoluene Cyclocybe aegerita 4-nitrobenzyl alcohol + H2O
-
 ?
1.11.2.1 benzaldehyde + H2O2
-
 Cyclocybe aegerita benzoic acid + H2O
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
1.11.2.1 toluene + H2O2 the initial product of toluene oxidation is benzyl alcohol, which then declines with concomitant production of benzaldehyde, which in turn declines with concomitant production of benzoic acid. The reactions proceed stepwise with total conversions of 93% for toluene Cyclocybe aegerita benzyl alcohol + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 APO
-
 Cyclocybe aegerita
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.2.1 Cyclocybe aegerita isoform II, isoelectric focusing
-
 5.6

General Information

EC Number General Information Comment Organism
1.11.2.1 physiological function APO-catalyzed reactions resemble the benzylic hydroxylations catalyzed by P450s via their H2O2-dependent peroxide shunt mechanism Cyclocybe aegerita