Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakamura, T.; Ichinose, H.; Wariishi, H.
    Cloning and heterologous expression of two aryl-aldehyde dehydrogenases from the white-rot basidiomycete Phanerochaete chrysosporium (2010), Biochem. Biophys. Res. Commun., 394, 470-475.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21 (DE3) pLysS cells Phanerodontia chrysosporium
1.2.1.29 PcALDH1 and PcALDH2 expressed as C-terminal histidine-tagged proteins in Escherichia coli cells Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 53300
-
isoform ALDH1, calculated from amino acid sequence Phanerodontia chrysosporium
1.2.1.3 55400
-
isoform ALDH2, calculated from amino acid sequence Phanerodontia chrysosporium
1.2.1.29 53300
-
PcALDH1, calculated from sequence Phanerodontia chrysosporium
1.2.1.29 54400
-
PcALDH2, calculated from sequence Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Phanerodontia chrysosporium
-
-
-
1.2.1.3 Phanerodontia chrysosporium ATCC 34541
-
-
-
1.2.1.29 Phanerodontia chrysosporium
-
white-rot basidiomycete
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 His Gravi-Trap column chromatography Phanerodontia chrysosporium
1.2.1.29 PcALDH1 and PcALDH2 recombinant protein purified with a His-tag affinity column Phanerodontia chrysosporium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 mycelium
-
Phanerodontia chrysosporium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4,5-trimethoxybenzoic acid + NADH + H+
-
?
1.2.1.3 3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dihydroxybenzoic acid + NADH + H+
-
?
1.2.1.3 3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
?
1.2.1.3 3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.3 4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.3 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
?
1.2.1.3 4-hydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 4-hydroxybenzoic acid + NADH + H+
-
?
1.2.1.3 benzaldehyde + NAD+ + H2O best substrate, isoform ALDH1 shows 2fold greater catalytic activity against benzaldehyde than against other substrates, while isoform ALDH2 shows no substrate preferences Phanerodontia chrysosporium benzoate + NADH + H+
-
?
1.2.1.3 vanillin + NAD+ + H2O
-
Phanerodontia chrysosporium vanillic acid + NADH + H+
-
?
1.2.1.29 3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4,5-trimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium ATCC 34541 3,4,5-trimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dihydroxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium ATCC 34541 3,4-dihydroxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium ATCC 34541 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 3,4-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium ATCC 34541 3,4-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium ATCC 34541 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
?
1.2.1.29 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O 4-hydroxy-3-methoxybenzaldehyde i.e. vanillin Phanerodontia chrysosporium 4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
?
1.2.1.29 4-hydroxybenzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium 4-hydroxybenzoic acid + NADH + H+
-
?
1.2.1.29 benzaldehyde + NAD+ + H2O
-
Phanerodontia chrysosporium benzoic acid + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH1 isoform Phanerodontia chrysosporium
1.2.1.3 ALDH2 isoform Phanerodontia chrysosporium
1.2.1.3 aryl-aldehyde dehydrogenases
-
Phanerodontia chrysosporium
1.2.1.29 aryl-aldehyde dehydrogenase
-
Phanerodontia chrysosporium
1.2.1.29 PcALDH1
-
Phanerodontia chrysosporium
1.2.1.29 PcALDH2
-
Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 10
-
-
Phanerodontia chrysosporium
1.2.1.29 10
-
-
Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.29 4 11 enzymatic reactions carried out at pH 4.0 show only 5–10% of the maximum activities observed at pH 10.0 Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 4
-
enzymatic reactions at pH 4.0 show only 5-10% of the maximum activities observed at pH 10.0 Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
Phanerodontia chrysosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.3 Phanerodontia chrysosporium isoform ALDH1, calculated from amino acid sequence
-
5.9
1.2.1.3 Phanerodontia chrysosporium isoform ALDH2, calculated from amino acid sequence
-
6.1
1.2.1.29 Phanerodontia chrysosporium PcALDH1, estimated from sequence
-
5.9
1.2.1.29 Phanerodontia chrysosporium PcALDH2, estimated from sequence
-
6.1

Expression

EC Number Organism Comment Expression
1.2.1.3 Phanerodontia chrysosporium both ALDH isoforms are translationally up-regulated in response to exogenous addition of vanillin up
1.2.1.29 Phanerodontia chrysosporium up-regulation of PcALDH1 and PcALDH2 in response to exogenous addition of vanillin, which is an abundant key intermediate in the lignin biodegradation process, basidiomycetes presumably respond to vanillin by activating and optimizing ligninolytic processes up

General Information

EC Number General Information Comment Organism
1.2.1.29 metabolism results suggest that PcALDHs metabolize aryl-aldehyde compounds generated during fungal degradation of lignin and various aromatic xenobiotics Phanerodontia chrysosporium