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Literature summary extracted from

  • Sugano, Y.; Ishii, Y.; Shoda, M.
    Role of H164 in a unique dye-decolorizing heme peroxidase DyP (2004), Biochem. Biophys. Res. Commun., 322, 126-132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli BL21(DE3) cells and in Aspergillus oryzae strain RD005 Thanatephorus cucumeris

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.19 H164A the specific activity of the purified mutant is 99.8% lower than that of recombinant DyP expressed in Escherichia coli Thanatephorus cucumeris
1.11.1.19 H166A the specific activity of the purified mutant is 95% lower than that of recombinant DyP expressed in Escherichia coli Thanatephorus cucumeris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.19 47000
-
 recombinant enzyme expressed in Escherichia coli, SDS-PAGE Thanatephorus cucumeris
1.11.1.19 47460
-
 recombinant enzyme expressed in Escherichia coli, deduced from amino acid sequence Thanatephorus cucumeris
1.11.1.19 50000
-
 recombinant enzyme expressed in Escherichia coli, gel filtration Thanatephorus cucumeris
1.11.1.19 58000
-
 recombinant enzyme expressed in Aspergillus oryzae, gel filtration Thanatephorus cucumeris
1.11.1.19 60000
-
 native enzyme, gel filtration Thanatephorus cucumeris

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Thanatephorus cucumeris
-
 strain Dec 1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.19 glycoprotein
-
 Thanatephorus cucumeris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 ammonium sulfate precipitation, QAE-Toyopearl column chromatography, Mono Q column chromatography, Poros PE column chromatography, and Superdex 75 gel filtration Thanatephorus cucumeris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.19 0.058
-
 recombinant enzyme from crude extract, at 30°C, pH 3.2 Thanatephorus cucumeris
1.11.1.19 834
-
 recombinant enzyme after 14385fold purification, at 30°C, pH 3.2 Thanatephorus cucumeris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ native DyP shows 9.3% activity, recombinant enzyme expressed in Escherichia coli shows 9.0% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 8.1% activity compared to Reactive Blue 5 Thanatephorus cucumeris ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ native DyP shows 9.2% activity, recombinant enzyme expressed in Escherichia coli shows 10% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 6.0% activity compared to Reactive Blue 5 Thanatephorus cucumeris ?
-
 ?
1.11.1.19 additional information native and recombinant DyPs do not oxidize veratryl alcohol Thanatephorus cucumeris ?
-
 ?
1.11.1.19 Reactive Black 5 + H2O2 + H+ native DyP shows 1.0% activity, recombinant enzyme expressed in Escherichia coli shows 0.9% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 1.0% activity compared to Reactive Blue 5 Thanatephorus cucumeris oxidized Reactive Black 5 + H2O
-
 ?
1.11.1.19 Reactive Blue 114 + H2O2 + H+ native DyP shows 4.4% activity, recombinant enzyme expressed in Escherichia coli shows 2.4% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 2.2% activity compared to Reactive Blue 5 Thanatephorus cucumeris oxidized Reactive Blue 114 + H2O
-
 ?
1.11.1.19 Reactive Blue 19 + H2O2 + H+ native DyP shows 119% activity, recombinant enzyme expressed in Escherichia coli shows 102% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 101% activity compared to Reactive Blue 5 Thanatephorus cucumeris oxidized Reactive Blue 19 + H2O
-
 ?
1.11.1.19 Reactive Blue 21 + H2O2 + H+ native DyP shows 26% activity, recombinant enzyme expressed in Escherichia coli shows 32% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 25% activity compared to Reactive Blue 5 Thanatephorus cucumeris oxidized Reactive Blue 21 + H2O
-
 ?
1.11.1.19 Reactive Blue 5 + H2O2 + H+ native DyP and recombinant enzymes expressed in Escherichia coli and Aspergillus oryzae show 100% activity Thanatephorus cucumeris oxidized Reactive Blue 5 + H2O
-
 ?
1.11.1.19 Reactive Orange 13 + H2O2 + H+ native DyP shows 0.22% activity compared to Reactive Blue 5, recombinant enzymes expressed in Escherichia coli or in Aspergillus oryzae show no activity with Reactive Orange 13 Thanatephorus cucumeris oxidized Reactive Orange 13 + H2O
-
 ?
1.11.1.19 Reactive Red 120 + H2O2 + H+ native DyP shows 0.96% activity, recombinant enzyme expressed in Escherichia coli shows 0.61% activity compared to Reactive Blue 5, and recombinant enzyme expressed in Aspergillus oryzae shows no activity with Reactive Red 120 Thanatephorus cucumeris oxidized Reactive Red 120 + H2O
-
 ?
1.11.1.19 Reactive Red 33 + H2O2 + H+ native DyP shows 2.3 activity, recombinant enzyme expressed in Escherichia coli shows 1.8% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 2.2% activity compared to Reactive Blue 5 Thanatephorus cucumeris oxidized Reactive Red 33 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Thanatephorus cucumeris
1.11.1.19 DyP
-
 Thanatephorus cucumeris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 30 70 recombinant DyP expressed in Escherichia coli does not lose its activity at 30°C and 40°C. The activity is loweres slightly to 83% of full activity after heating 1 h at 50°C. In contrast, the activity drastically declines at 60°C and 70°C. The residual activity is 4% and 10% of full activity after heating 2 h at 60°C and 15 min at 70°C, respectively. The activity of native DyP after 1 h heating at 60°C is 92% of full activity whereas that of recombinant DyPs expressed in Escherichia coli and Aspergillus oryzae are only 42%, and 44%, respectively Thanatephorus cucumeris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 3.2
-
-
 Thanatephorus cucumeris

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Thanatephorus cucumeris