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Literature summary extracted from

  • Sakuraba, H.; Yokono, K.; Yoneda, K.; Watanabe, A.; Asada, Y.; Satomura, T.; Yabutani, T.; Motonaka, J.; Ohshima, T.
    Catalytic properties and crystal structure of quinoprotein aldose sugar dehydrogenase from hyperthermophilic archaeon Pyrobaculum aerophilum (2010), Arch. Biochem. Biophys., 502, 81-88.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells Pyrobaculum aerophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.5.2 apoform and pyrroloquinoline quinone-bound holoenzyme, sitting drop vapor diffusion method, using 1.5 M ammonium sulfate and 0.1 M Tris/HCl buffer (pH 8.4), at 25°C Pyrobaculum aerophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.2 methylhydrazine
-
 Pyrobaculum aerophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 390
-
 D-galactose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 390
-
 D-fructose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 500
-
 D-xylose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 600
-
 maltose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 680
-
 D-glucose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 910
-
 D-mannose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 26000
-
 gel filtration Pyrobaculum aerophilum
1.1.5.2 32000
-
 1 * 32000, SDS-PAGE Pyrobaculum aerophilum
1.1.5.2 38452
-
 1 * 38452, calculated from amino acid sequence Pyrobaculum aerophilum
1.1.5.2 40000
-
 dynamic light scattering Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + pyrroloquinoline quinone Pyrobaculum aerophilum
-
 D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Pyrobaculum aerophilum Q8ZUN8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 HiPrep 16/10 SP XL column chromatography Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 0.77
-
 purified enzyme,in 50 mM Bis–Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-cellobiose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum D-cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-fructose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-galactose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-glucose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-glucose + pyrroloquinoline quinone
-
 Pyrobaculum aerophilum D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-mannose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-ribose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 D-xylose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 L-arabinose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.2 maltose + 2,6-dichloroindophenol
-
 Pyrobaculum aerophilum maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 monomer 1 * 32000, SDS-PAGE Pyrobaculum aerophilum
1.1.5.2 monomer 1 * 38452, calculated from amino acid sequence Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 GDH
-
 Pyrobaculum aerophilum
1.1.5.2 quinoprotein aldose sugar dehydrogenase
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 75
-
 above 75°C Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.2 100
-
 the enzyme is extremely thermostable, and the activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 100 °C for 10 min, while the apoenzyme retains full activity at up to 80°C under the same conditions Pyrobaculum aerophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 2.3
-
 maltose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 3.1
-
 D-mannose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 3.2
-
 D-xylose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 4.1
-
 D-glucose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 4.3
-
 D-galactose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 8
-
 the optimum pH for D-glucose oxidation at 50°C is around pH 8.0 Pyrobaculum aerophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.2 3 11 when heated at 70°C for 30 min, the apo- and holoenzymes do not lose activity at pHs in the ranges of 4-11 and 3-11, respectively Pyrobaculum aerophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone
-
 Pyrobaculum aerophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.5.2 400
-
 D-fructose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 1700
-
 D-ribose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 2900
-
 D-cellobiose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 3400
-
 D-mannose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 3900
-
 maltose in 50 mM Bis–-ris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 4100
-
 L-arabinose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 6100
-
 D-glucose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 6500
-
 D-xylose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum
1.1.5.2 11000
-
 D-galactose in 50 mM Bis-Tris propane (pH 8.0), at 50°C Pyrobaculum aerophilum