EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.30 | mutant enzyme E145A in complex with the substrate analogue alpha,beta-imido-dUTP and mutant enzyme E145Q in complex with diphosphate, hanging drop vapor diffusion method, using 0.2 M potassium citrate (E135Q) or 0.2 M potassium hydrogen phosphate (E145A) and 20% (w/v) PEG3350 | Methanocaldococcus jannaschii |
3.6.1.23 | hanging drop vapor diffusion method, using 0.2 M potassium hydrogen phosphate and 20% (w/v) PEG3350 for mutant enzyme E145A complexed with alpha,beta-imido-dUTP, or 0.2 M potassium citrate and 20% (w/v) PEG3350 for mutant enzyme E145Q complexed with diphosphate | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.30 | E145A | the mutant enzyme is defect in the deaminase reaction | Methanocaldococcus jannaschii |
3.5.4.30 | E145Q | the mutant enzyme is defect in the deaminase reaction | Methanocaldococcus jannaschii |
3.6.1.23 | E145A | the mutant shows reduced specific activity towards dUTP | Methanocaldococcus jannaschii |
3.6.1.23 | E145Q | the mutant shows reduced specific activity towards dUTP | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.30 | alpha,beta-imido-dUTP | - |
Methanocaldococcus jannaschii | |
3.5.4.30 | dTTP | - |
Methanocaldococcus jannaschii | |
3.6.1.23 | alpha,beta-imido-dUTP | - |
Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required for activity | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.30 | dCTP + H2O | Methanocaldococcus jannaschii | - |
dUMP + diphosphate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.30 | Methanocaldococcus jannaschii | Q57872 | - |
- |
3.6.1.23 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.30 | - |
Methanocaldococcus jannaschii |
3.6.1.23 | - |
Methanocaldococcus jannaschii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 0.52 | - |
mutant enzyme E145Q, in 50 mM HEPES, pH 6.8, at 50°C | Methanocaldococcus jannaschii |
3.6.1.23 | 1.33 | - |
mutant enzyme E145A, in 50 mM HEPES, pH 6.8, at 50°C | Methanocaldococcus jannaschii |
3.6.1.23 | 8.8 | - |
wild type enzyme, in 50 mM HEPES, pH 6.8, at 50°C | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.30 | dCTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate + NH3 | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.30 | dCTP deaminase-dUTPase | bifunctional enzyme | Methanocaldococcus jannaschii |
3.6.1.23 | dCTP deaminase-dUTPase | bifunctional enzyme | Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.4.30 | metabolism | the bifunctional dCTP deaminase-dUTPase coordinates the deamination and triphosphate cleavage reactions | Methanocaldococcus jannaschii |