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Literature summary extracted from

  • Auburger, G.; Winter, J.
    Purification and chracterization of benzoyl-CoA ligase from a syntrophic, benzoate-degrading, anaerobic mixed culture (1992), Appl. Microbiol. Biotechnol., 37, 789-795.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.25 CoCl2
-
 Anaerobic bacterium
6.2.1.25 CuCl2 CuCl2 Anaerobic bacterium
6.2.1.25 EDTA
-
 Anaerobic bacterium
6.2.1.25 FeCl2
-
 Anaerobic bacterium
6.2.1.25 NiCl2
-
 Anaerobic bacterium
6.2.1.25 Zn2+ ZnCl2 Anaerobic bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.25 0.03
-
 benzoate
-
 Anaerobic bacterium
6.2.1.25 0.06
-
 CoASH
-
 Anaerobic bacterium
6.2.1.25 0.56
-
 ATP
-
 Anaerobic bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.25 Mg2+ required Anaerobic bacterium
6.2.1.25 Mg2+ optimal concentration: 5 mM MgCl2 Anaerobic bacterium
6.2.1.25 Mn2+ can replace Mg2+ Anaerobic bacterium
6.2.1.25 Mn2+ optimal concentration: 5 mM MnCl2, 85% of the efficiency compared to MgCl2 Anaerobic bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.25 58000
-
 7 or 8 * 58000, SDS-PAGE Anaerobic bacterium

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.25 Anaerobic bacterium
-
 a strictly anaerobic, obligate synthrophic mixed culture
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.25
-
 Anaerobic bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.25 additional information
-
-
 Anaerobic bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.25 ATP + 2-fluorobenzoate + CoA 93% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 3-fluorobenzoate + CoA 56% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + 4-fluorobenzoate + CoA 75% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
6.2.1.25 ATP + benzoate + CoA
-
 Anaerobic bacterium AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.25 ATP + isonicotinate + CoA 29% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + isonicotinyl-CoA
-
 ?
6.2.1.25 ATP + nicotinate + CoA 11% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + nicotinyl-CoA
-
 ?
6.2.1.25 ATP + picolinate + CoA 46% of the activity relative to benzoate Anaerobic bacterium AMP + diphosphate + picolinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.25 ? 7 or 8 * 58000, SDS-PAGE Anaerobic bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.25 37
-
-
 Anaerobic bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.25 22 45 22°C: about 40% of maximal activity, 45°C: about 20% of maximal activity Anaerobic bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.25 8
-
-
 Anaerobic bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.25 7 9 7: about 40% of maximal activity, 9: about 30% of maximal activity Anaerobic bacterium