BRENDA - Enzyme Database show

Characteristics and biotechnological applications of microbial cholesterol oxidases

Doukyu, N.; Appl. Microbiol. Biotechnol. 83, 825-837 (2009)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Brevibacterium sterolicum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Corynebacterium cholesterolicum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptomyces sp.
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptomyces violascens
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptoverticillium cholesterolieum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
uncultured gamma proteobacterium
1.1.3.6
butanol
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
butanol
the enzyme is inactivated by the addition of 50% (v/v) of butanol after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
CuSO4
significant inhibition
Streptoverticillium cholesterolieum
1.1.3.6
Dimethylsulfoxide
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
Dimethylsulfoxide
the enzyme is inactivated by the addition of 50% (v/v) of dimethylsulfoxide after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
ethyl acetate
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
ethyl acetate
the enzyme is inactivated by the addition of 50% (v/v) of ethyl acetate after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
Fe2+
markedly inhibited by Fe2+
Pseudomonas sp.
1.1.3.6
FeCl3
markedly inhibited by FeCl3
Streptomyces violascens
1.1.3.6
FeSO4
markedly inhibited by FeSO4
Streptomyces violascens
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Brevibacterium sterolicum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Corynebacterium cholesterolicum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Pimelobacter simplex
1.1.3.6
Hg2+
markedly inhibited by Hg2+
Pseudomonas sp.
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptomyces sp.
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptomyces violascens
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptoverticillium cholesterolieum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
uncultured gamma proteobacterium
1.1.3.6
p-chloromercuribenzoic acid
the addition of p-chloromercuribenzoic acid partially reduces the activity of the enzyme
Pimelobacter simplex
1.1.3.6
Zn2+
markedly inhibited by Zn2+
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.6
0.0184
-
cholesterol
pH 7.0, temperature not specified in the publication
Nocardia sp.
1.1.3.6
0.0188
-
cholesterol
pH and temperature not specified in the publication
Rhodococcus erythropolis
1.1.3.6
0.0262
-
cholesterol
pH and temperature not specified in the publication
Chromobacterium sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.6
Mn2+
partially activated by the addition of Mn2+
Pseudomonas sp.
1.1.3.6
Mn2+
partially activated by the addition of Mn2+
uncultured gamma proteobacterium
1.1.3.6
additional information
Ag+ scarcely influences the activity of the enzyme
Chromobacterium sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.6
53000
-
-
Schizophyllum commune
1.1.3.6
55000
-
-
Brevibacterium sterolicum
1.1.3.6
55000
-
-
Streptomyces sp.
1.1.3.6
56000
-
-
Pseudomonas sp.
1.1.3.6
56000
-
-
Streptoverticillium cholesterolieum
1.1.3.6
57000
-
-
Corynebacterium cholesterolicum
1.1.3.6
57000
-
-
Pimelobacter simplex
1.1.3.6
58000
-
-
Chromobacterium sp.
1.1.3.6
58000
-
-
Streptomyces sp.
1.1.3.6
58000
-
-
uncultured gamma proteobacterium
1.1.3.6
60000
-
-
Burkholderia cepacia
1.1.3.6
60000
-
-
Streptomyces fradiae
1.1.3.6
63000
-
-
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.6
beta-cholestanol + O2
Streptomyces violascens
101% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Brevibacterium sterolicum
13% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Burkholderia cepacia
69% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Streptomyces sp.
75% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Pseudomonas sp.
78% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
uncultured gamma proteobacterium
79% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Chromobacterium sp.
95% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Streptoverticillium cholesterolieum
96% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Pseudomonas sp. COX629
78% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
uncultured gamma proteobacterium Y-134
79% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-sitosterol + O2
Brevibacterium sterolicum
20% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
uncultured gamma proteobacterium
29% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptoverticillium cholesterolieum
39% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Corynebacterium cholesterolicum
50% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptomyces violascens
64% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptomyces sp.
72% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Chromobacterium sp.
80% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Burkholderia cepacia
84% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Burkholderia cepacia ST-200
84% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
uncultured gamma proteobacterium Y-134
29% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Brevibacterium sterolicum
10% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Corynebacterium cholesterolicum
12% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptoverticillium cholesterolieum
17% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
uncultured gamma proteobacterium
32% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Chromobacterium sp.
36% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptomyces sp.
37% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pseudomonas sp.
40% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pimelobacter simplex
47% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptomyces violascens
50% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Burkholderia cepacia
59% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pseudomonas sp. COX629
40% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
uncultured gamma proteobacterium Y-134
32% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas aeruginosa
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Arthrobacter sp.
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces fradiae
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia rhodochrous
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia pseudomallei
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus hoagii
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces avermitilis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces griseus
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces natalensis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces sp.
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium urealyticum
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia farcinica
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium leprae
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium tuberculosis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces coelicolor
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus erythropolis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia thailandensis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Paraburkholderia xenovorans
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pimelobacter simplex
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Schizophyllum commune
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces violascens
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium cholesterolicum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
uncultured gamma proteobacterium
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia cepacia
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Chromobacterium sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptoverticillium cholesterolieum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Brevibacterium sterolicum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia cepacia ST-200
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus hoagii 103S
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia farcinica IFM 10152
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas sp. COX629
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
uncultured gamma proteobacterium Y-134
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium urealyticum DSM 7109
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium tuberculosis H37Rv
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Arthrobacter sp. IM79
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Burkholderia cepacia
16% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptomyces sp.
24% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Chromobacterium sp.
27% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Schizophyllum commune
28% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptomyces violascens
37% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Brevibacterium sterolicum
41% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptoverticillium cholesterolieum
48% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Corynebacterium cholesterolicum
5% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Burkholderia cepacia ST-200
16% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
epiandrosterone + O2
Nocardia sp.
1% activity compared to cholesterol
?
-
-
?
1.1.3.6
epiandrosterone + O2
Burkholderia cepacia
10% activity compared to cholesterol
?
-
-
?
1.1.3.6
epiandrosterone + O2
Chromobacterium sp.
15% activity compared to cholesterol
?
-
-
?
1.1.3.6
ergosterol + O2
Streptomyces sp.
12% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Chromobacterium sp.
12% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Streptomyces violascens
14% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pimelobacter simplex
17% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Burkholderia cepacia
20% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Corynebacterium cholesterolicum
4% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Streptoverticillium cholesterolieum
5% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pseudomonas sp.
51% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Burkholderia cepacia ST-200
20% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pseudomonas sp. COX629
51% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
additional information
Pseudomonas sp.
no activity with dehydroepiandrosterone
?
-
-
-
1.1.3.6
additional information
Schizophyllum commune
no activity with ergosterol
?
-
-
-
1.1.3.6
additional information
Brevibacterium sterolicum
no activity with ergosterol
?
-
-
-
1.1.3.6
additional information
uncultured gamma proteobacterium
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
?
-
-
-
1.1.3.6
additional information
Rhodococcus erythropolis
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
?
-
-
-
1.1.3.6
additional information
Pseudomonas sp. COX629
no activity with dehydroepiandrosterone
?
-
-
-
1.1.3.6
additional information
uncultured gamma proteobacterium Y-134
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
?
-
-
-
1.1.3.6
pregnenolone + O2
Schizophyllum commune
20% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Brevibacterium sterolicum
22% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Burkholderia cepacia
32% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptoverticillium cholesterolieum
37% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Corynebacterium cholesterolicum
38% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptomyces violascens
42% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Nocardia sp.
6% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Chromobacterium sp.
95% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptomyces sp.
99% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Burkholderia cepacia ST-200
32% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.1.3.6
Acetone
the enzyme is unstable in 50% (v/v) acetone; the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Butanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
DMSO
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Emal 20CM
the enzyme is relatively tolerant to treatment with Emal 20CM even at 60°C
Chromobacterium sp.
1.1.3.6
Ethanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Ethyl acetate
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
isopropanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Methanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
propan-2-ol
the activity of the enzyme is rapidly inactivated in the presence of 30% (v/v) propan-2-ol at 25°C
Brevibacterium sterolicum
1.1.3.6
SDS
the enzyme is relatively tolerant to treatment with SDS after incubation for 1 h at 30°C
Chromobacterium sp.
1.1.3.6
sodium cholate
the enzyme is stable at 50% (v/v) sodium cholate after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
sodium cholate
the enzyme is stable in sodium cholate after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
sodium cholate
the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C
uncultured gamma proteobacterium
1.1.3.6
sodium dodecyl sarcosinate
the enzyme is relatively tolerant to treatment with sodium dodecyl sarcosinate even at 60°C
Chromobacterium sp.
1.1.3.6
sodium laurylbenzenesulfonate
the enzyme is relatively tolerant to treatment with sodium laurylbenzenesulfonate after incubation for 1 h at 30°C
Chromobacterium sp.
1.1.3.6
sodium polyoxyethylene alkyl ether sulfate
the enzyme is stable at 50% (v/v) sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
sodium polyoxyethylene alkyl ether sulfate
the enzyme is stable in sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-100
the enzyme is stable at 50% (v/v) Triton X-100 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Triton X-100
the enzyme is stable in Triton X-100 after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-405
the enzyme is stable at 50% (v/v) Triton X-405 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Triton X-405
the enzyme is stable in Triton X-405 after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-405
the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C
uncultured gamma proteobacterium
1.1.3.6
Tween 20
the enzyme is stable at 50% (v/v) Tween 20 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Tween 20
the enzyme is stable in Tween 20 after incubation for 1 h at 30°C
Rhodococcus erythropolis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.6
Arthrobacter sp.
-
-
-
1.1.3.6
Arthrobacter sp.
Q56DL0
-
-
1.1.3.6
Arthrobacter sp. IM79
-
-
-
1.1.3.6
Brevibacterium sterolicum
P22637
-
-
1.1.3.6
Burkholderia cepacia
Q93RE1
-
-
1.1.3.6
Burkholderia cepacia ST-200
Q93RE1
-
-
1.1.3.6
Burkholderia pseudomallei
-
-
-
1.1.3.6
Burkholderia thailandensis
Q2T0X3
-
-
1.1.3.6
Chromobacterium sp.
B5MGF8
-
-
1.1.3.6
Corynebacterium cholesterolicum
-
-
-
1.1.3.6
Corynebacterium urealyticum
B1VGH1
-
-
1.1.3.6
Corynebacterium urealyticum DSM 7109
B1VGH1
-
-
1.1.3.6
Mycobacterium leprae
Q59530
-
-
1.1.3.6
Mycobacterium tuberculosis
P9WMV9
-
-
1.1.3.6
Mycobacterium tuberculosis H37Rv
P9WMV9
-
-
1.1.3.6
Nocardia farcinica
Q5YRJ2
-
-
1.1.3.6
Nocardia farcinica IFM 10152
Q5YRJ2
-
-
1.1.3.6
Nocardia rhodochrous
-
-
-
1.1.3.6
Nocardia sp.
-
-
-
1.1.3.6
Paraburkholderia xenovorans
Q13UN0
-
-
1.1.3.6
Pimelobacter simplex
-
-
-
1.1.3.6
Pseudomonas aeruginosa
-
-
-
1.1.3.6
Pseudomonas sp.
-
-
-
1.1.3.6
Pseudomonas sp. COX629
-
-
-
1.1.3.6
Rhodococcus erythropolis
A9QAE7
-
-
1.1.3.6
Rhodococcus hoagii
E4WKM2
-
-
1.1.3.6
Rhodococcus hoagii 103S
E4WKM2
-
-
1.1.3.6
Schizophyllum commune
-
-
-
1.1.3.6
Streptomyces avermitilis
Q93H76
-
-
1.1.3.6
Streptomyces coelicolor
Q9L0H6
-
-
1.1.3.6
Streptomyces fradiae
-
-
-
1.1.3.6
Streptomyces griseus
A7RA76
-
-
1.1.3.6
Streptomyces natalensis
Q9EW96
-
-
1.1.3.6
Streptomyces sp.
P12676
-
-
1.1.3.6
Streptomyces sp.
Q55020
-
-
1.1.3.6
Streptomyces violascens
-
-
-
1.1.3.6
Streptoverticillium cholesterolieum
-
-
-
1.1.3.6
uncultured gamma proteobacterium
-
-
-
1.1.3.6
uncultured gamma proteobacterium Y-134
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.6
beta-cholestanol + O2
101% activity compared to cholesterol
710959
Streptomyces violascens
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
13% activity compared to cholesterol
710959
Brevibacterium sterolicum
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
69% activity compared to cholesterol
710959
Burkholderia cepacia
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
75% activity compared to cholesterol
710959
Streptomyces sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
78% activity compared to cholesterol
710959
Pseudomonas sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
79% activity compared to cholesterol
710959
uncultured gamma proteobacterium
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
95% activity compared to cholesterol
710959
Chromobacterium sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
96% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
78% activity compared to cholesterol
710959
Pseudomonas sp. COX629
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
79% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
?
-
-
-
?
1.1.3.6
beta-sitosterol + O2
20% activity compared to cholesterol
710959
Brevibacterium sterolicum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
29% activity compared to cholesterol
710959
uncultured gamma proteobacterium
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
39% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
50% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
64% activity compared to cholesterol
710959
Streptomyces violascens
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
72% activity compared to cholesterol
710959
Streptomyces sp.
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
80% activity compared to cholesterol
710959
Chromobacterium sp.
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
84% activity compared to cholesterol
710959
Burkholderia cepacia
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
84% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
29% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
10% activity compared to cholesterol
710959
Brevibacterium sterolicum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
12% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
17% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
32% activity compared to cholesterol
710959
uncultured gamma proteobacterium
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
36% activity compared to cholesterol
710959
Chromobacterium sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
37% activity compared to cholesterol
710959
Streptomyces sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
40% activity compared to cholesterol
710959
Pseudomonas sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
47% activity compared to cholesterol
710959
Pimelobacter simplex
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
50% activity compared to cholesterol
710959
Streptomyces violascens
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
59% activity compared to cholesterol
710959
Burkholderia cepacia
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
40% activity compared to cholesterol
710959
Pseudomonas sp. COX629
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
32% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Pseudomonas aeruginosa
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Arthrobacter sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces fradiae
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia rhodochrous
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Burkholderia pseudomallei
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus hoagii
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces avermitilis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces griseus
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces natalensis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Corynebacterium urealyticum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia farcinica
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium leprae
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium tuberculosis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces coelicolor
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus erythropolis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Burkholderia thailandensis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Paraburkholderia xenovorans
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pseudomonas sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pimelobacter simplex
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Nocardia sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Schizophyllum commune
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptomyces violascens
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Corynebacterium cholesterolicum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
uncultured gamma proteobacterium
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptomyces sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Burkholderia cepacia
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Chromobacterium sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptoverticillium cholesterolieum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Brevibacterium sterolicum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Burkholderia cepacia ST-200
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus hoagii 103S
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia farcinica IFM 10152
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pseudomonas sp. COX629
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
uncultured gamma proteobacterium Y-134
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Corynebacterium urealyticum DSM 7109
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium tuberculosis H37Rv
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Arthrobacter sp. IM79
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
16% activity compared to cholesterol
710959
Burkholderia cepacia
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
24% activity compared to cholesterol
710959
Streptomyces sp.
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
27% activity compared to cholesterol
710959
Chromobacterium sp.
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
28% activity compared to cholesterol
710959
Schizophyllum commune
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
37% activity compared to cholesterol
710959
Streptomyces violascens
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
41% activity compared to cholesterol
710959
Brevibacterium sterolicum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
48% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
5% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
16% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
epiandrosterone + O2
1% activity compared to cholesterol
710959
Nocardia sp.
?
-
-
-
?
1.1.3.6
epiandrosterone + O2
10% activity compared to cholesterol
710959
Burkholderia cepacia
?
-
-
-
?
1.1.3.6
epiandrosterone + O2
15% activity compared to cholesterol
710959
Chromobacterium sp.
?
-
-
-
?
1.1.3.6
ergosterol + O2
12% activity compared to cholesterol
710959
Streptomyces sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
12% activity compared to cholesterol
710959
Chromobacterium sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
14% activity compared to cholesterol
710959
Streptomyces violascens
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
17% activity compared to cholesterol
710959
Pimelobacter simplex
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
20% activity compared to cholesterol
710959
Burkholderia cepacia
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
4% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
5% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
51% activity compared to cholesterol
710959
Pseudomonas sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
20% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
51% activity compared to cholesterol
710959
Pseudomonas sp. COX629
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
additional information
no activity with dehydroepiandrosterone
710959
Pseudomonas sp.
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol
710959
Schizophyllum commune
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol
710959
Brevibacterium sterolicum
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
710959
uncultured gamma proteobacterium
?
-
-
-
-
1.1.3.6
additional information
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
710959
Rhodococcus erythropolis
?
-
-
-
-
1.1.3.6
additional information
no activity with dehydroepiandrosterone
710959
Pseudomonas sp. COX629
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
710959
uncultured gamma proteobacterium Y-134
?
-
-
-
-
1.1.3.6
pregnenolone + O2
20% activity compared to cholesterol
710959
Schizophyllum commune
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
22% activity compared to cholesterol
710959
Brevibacterium sterolicum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
32% activity compared to cholesterol
710959
Burkholderia cepacia
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
37% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
38% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
42% activity compared to cholesterol
710959
Streptomyces violascens
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
6% activity compared to cholesterol
710959
Nocardia sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
95% activity compared to cholesterol
710959
Chromobacterium sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
99% activity compared to cholesterol
710959
Streptomyces sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
32% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
5-pregnen-3,20-dione + H2O2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.6
30
-
-
Nocardia rhodochrous
1.1.3.6
40
42
-
Corynebacterium cholesterolicum
1.1.3.6
40
50
-
Arthrobacter sp.
1.1.3.6
40
-
-
Rhodococcus hoagii
1.1.3.6
45
50
-
Streptomyces sp.
1.1.3.6
50
-
-
Brevibacterium sterolicum
1.1.3.6
50
-
-
Pimelobacter simplex
1.1.3.6
50
-
-
Streptomyces violascens
1.1.3.6
60
-
-
Burkholderia cepacia
1.1.3.6
65
-
-
Chromobacterium sp.
1.1.3.6
70
-
-
Streptomyces fradiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.6
50
60
96% activity after 30 min at 50°C, 6% activity after 30 min at 60°C
Corynebacterium cholesterolicum
1.1.3.6
55
-
less than 50% activity after 15 min at 55°C
Streptomyces sp.
1.1.3.6
60
85
the enzyme retains more than 90% of its original activity after incubation for 240 min at 60°C, 90% activity after 1 h at 80°C, and 80% activity after 30 min at 85°C
Chromobacterium sp.
1.1.3.6
70
80
70% residual activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C
Burkholderia cepacia
1.1.3.6
70
80
70% activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C
Pseudomonas sp.
1.1.3.6
70
-
10% activity after 1 h at 70°C
Streptomyces fradiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.6
5
-
-
Schizophyllum commune
1.1.3.6
6.5
7
-
Streptomyces sp.
1.1.3.6
6.5
-
-
uncultured gamma proteobacterium
1.1.3.6
6.8
8
-
Burkholderia cepacia
1.1.3.6
7
7.5
-
Chromobacterium sp.
1.1.3.6
7
7.5
-
Corynebacterium cholesterolicum
1.1.3.6
7
7.5
-
Streptoverticillium cholesterolieum
1.1.3.6
7
-
-
Nocardia rhodochrous
1.1.3.6
7
-
-
Nocardia sp.
1.1.3.6
7
-
-
Pseudomonas sp.
1.1.3.6
7
-
-
Streptomyces fradiae
1.1.3.6
7
-
-
Streptomyces sp.
1.1.3.6
7
-
-
Streptomyces violascens
1.1.3.6
7.5
8.5
-
Arthrobacter sp.
1.1.3.6
7.5
-
-
Brevibacterium sterolicum
1.1.3.6
7.5
-
-
Pimelobacter simplex
1.1.3.6
8
-
-
Rhodococcus hoagii
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.6
3
11
30°C, 1 h
Chromobacterium sp.
1.1.3.6
4
10
37°C, 30 min
Brevibacterium sterolicum
1.1.3.6
4
10
4°C, 4 h
Streptomyces fradiae
1.1.3.6
4
11
30°C, 1 h
Burkholderia cepacia
1.1.3.6
4
11
60°C, 1 min
Pseudomonas sp.
1.1.3.6
4
12.5
22°C, 1 h
Streptoverticillium cholesterolieum
1.1.3.6
4
8
25°C, 20 h
Streptomyces sp.
1.1.3.6
5
7.5
60°C, 1 h
uncultured gamma proteobacterium
1.1.3.6
5
9
30°C, 1 h
Corynebacterium cholesterolicum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.6
FAD
dependent on; dependent on
Arthrobacter sp.
1.1.3.6
FAD
dependent on
Brevibacterium sterolicum
1.1.3.6
FAD
dependent on
Burkholderia cepacia
1.1.3.6
FAD
dependent on
Burkholderia pseudomallei
1.1.3.6
FAD
dependent on
Burkholderia thailandensis
1.1.3.6
FAD
dependent on
Chromobacterium sp.
1.1.3.6
FAD
dependent on
Corynebacterium cholesterolicum
1.1.3.6
FAD
dependent on
Corynebacterium urealyticum
1.1.3.6
FAD
dependent on
Mycobacterium leprae
1.1.3.6
FAD
dependent on
Mycobacterium tuberculosis
1.1.3.6
FAD
dependent on
Nocardia farcinica
1.1.3.6
FAD
dependent on
Nocardia rhodochrous
1.1.3.6
FAD
dependent on
Nocardia sp.
1.1.3.6
FAD
dependent on
Paraburkholderia xenovorans
1.1.3.6
FAD
dependent on
Pimelobacter simplex
1.1.3.6
FAD
dependent on
Pseudomonas aeruginosa
1.1.3.6
FAD
dependent on
Pseudomonas sp.
1.1.3.6
FAD
dependent on
Rhodococcus erythropolis
1.1.3.6
FAD
dependent on
Rhodococcus hoagii
1.1.3.6
FAD
dependent on
Schizophyllum commune
1.1.3.6
FAD
dependent on
Streptomyces avermitilis
1.1.3.6
FAD
dependent on
Streptomyces coelicolor
1.1.3.6
FAD
dependent on
Streptomyces fradiae
1.1.3.6
FAD
dependent on
Streptomyces griseus
1.1.3.6
FAD
dependent on
Streptomyces natalensis
1.1.3.6
FAD
dependent on; dependent on
Streptomyces sp.
1.1.3.6
FAD
dependent on
Streptomyces violascens
1.1.3.6
FAD
dependent on
Streptoverticillium cholesterolieum
1.1.3.6
FAD
dependent on
uncultured gamma proteobacterium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.6
FAD
dependent on
Arthrobacter sp.
1.1.3.6
FAD
dependent on
Brevibacterium sterolicum
1.1.3.6
FAD
dependent on
Burkholderia cepacia
1.1.3.6
FAD
dependent on
Burkholderia pseudomallei
1.1.3.6
FAD
dependent on
Burkholderia thailandensis
1.1.3.6
FAD
dependent on
Chromobacterium sp.
1.1.3.6
FAD
dependent on
Corynebacterium cholesterolicum
1.1.3.6
FAD
dependent on
Corynebacterium urealyticum
1.1.3.6
FAD
dependent on
Mycobacterium leprae
1.1.3.6
FAD
dependent on
Mycobacterium tuberculosis
1.1.3.6
FAD
dependent on
Nocardia farcinica
1.1.3.6
FAD
dependent on
Nocardia rhodochrous
1.1.3.6
FAD
dependent on
Nocardia sp.
1.1.3.6
FAD
dependent on
Paraburkholderia xenovorans
1.1.3.6
FAD
dependent on
Pimelobacter simplex
1.1.3.6
FAD
dependent on
Pseudomonas aeruginosa
1.1.3.6
FAD
dependent on
Pseudomonas sp.
1.1.3.6
FAD
dependent on
Rhodococcus erythropolis
1.1.3.6
FAD
dependent on
Rhodococcus hoagii
1.1.3.6
FAD
dependent on
Schizophyllum commune
1.1.3.6
FAD
dependent on
Streptomyces avermitilis
1.1.3.6
FAD
dependent on
Streptomyces coelicolor
1.1.3.6
FAD
dependent on
Streptomyces fradiae
1.1.3.6
FAD
dependent on
Streptomyces griseus
1.1.3.6
FAD
dependent on
Streptomyces natalensis
1.1.3.6
FAD
dependent on
Streptomyces sp.
1.1.3.6
FAD
dependent on
Streptomyces violascens
1.1.3.6
FAD
dependent on
Streptoverticillium cholesterolieum
1.1.3.6
FAD
dependent on
uncultured gamma proteobacterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Brevibacterium sterolicum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Corynebacterium cholesterolicum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptomyces sp.
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptomyces violascens
1.1.3.6
AgNO3
markedly inhibited by AgNO3
Streptoverticillium cholesterolieum
1.1.3.6
AgNO3
markedly inhibited by AgNO3
uncultured gamma proteobacterium
1.1.3.6
butanol
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
butanol
the enzyme is inactivated by the addition of 50% (v/v) of butanol after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
CuSO4
significant inhibition
Streptoverticillium cholesterolieum
1.1.3.6
Dimethylsulfoxide
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
Dimethylsulfoxide
the enzyme is inactivated by the addition of 50% (v/v) of dimethylsulfoxide after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
ethyl acetate
the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C
Nocardia sp.
1.1.3.6
ethyl acetate
the enzyme is inactivated by the addition of 50% (v/v) of ethyl acetate after incubation for 24 h at 37°C
Rhodococcus erythropolis
1.1.3.6
Fe2+
markedly inhibited by Fe2+
Pseudomonas sp.
1.1.3.6
FeCl3
markedly inhibited by FeCl3
Streptomyces violascens
1.1.3.6
FeSO4
markedly inhibited by FeSO4
Streptomyces violascens
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Brevibacterium sterolicum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Corynebacterium cholesterolicum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Pimelobacter simplex
1.1.3.6
Hg2+
markedly inhibited by Hg2+
Pseudomonas sp.
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptomyces sp.
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptomyces violascens
1.1.3.6
Hg2+
markedly inhibited by HgCl2
Streptoverticillium cholesterolieum
1.1.3.6
Hg2+
markedly inhibited by HgCl2
uncultured gamma proteobacterium
1.1.3.6
p-chloromercuribenzoic acid
the addition of p-chloromercuribenzoic acid partially reduces the activity of the enzyme
Pimelobacter simplex
1.1.3.6
Zn2+
markedly inhibited by Zn2+
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.6
0.0184
-
cholesterol
pH 7.0, temperature not specified in the publication
Nocardia sp.
1.1.3.6
0.0188
-
cholesterol
pH and temperature not specified in the publication
Rhodococcus erythropolis
1.1.3.6
0.0262
-
cholesterol
pH and temperature not specified in the publication
Chromobacterium sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.6
Mn2+
partially activated by the addition of Mn2+
Pseudomonas sp.
1.1.3.6
Mn2+
partially activated by the addition of Mn2+
uncultured gamma proteobacterium
1.1.3.6
additional information
Ag+ scarcely influences the activity of the enzyme
Chromobacterium sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.6
53000
-
-
Schizophyllum commune
1.1.3.6
55000
-
-
Brevibacterium sterolicum
1.1.3.6
55000
-
-
Streptomyces sp.
1.1.3.6
56000
-
-
Pseudomonas sp.
1.1.3.6
56000
-
-
Streptoverticillium cholesterolieum
1.1.3.6
57000
-
-
Corynebacterium cholesterolicum
1.1.3.6
57000
-
-
Pimelobacter simplex
1.1.3.6
58000
-
-
Chromobacterium sp.
1.1.3.6
58000
-
-
Streptomyces sp.
1.1.3.6
58000
-
-
uncultured gamma proteobacterium
1.1.3.6
60000
-
-
Burkholderia cepacia
1.1.3.6
60000
-
-
Streptomyces fradiae
1.1.3.6
63000
-
-
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.6
beta-cholestanol + O2
Streptomyces violascens
101% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Brevibacterium sterolicum
13% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Burkholderia cepacia
69% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Streptomyces sp.
75% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Pseudomonas sp.
78% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
uncultured gamma proteobacterium
79% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Chromobacterium sp.
95% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Streptoverticillium cholesterolieum
96% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
Pseudomonas sp. COX629
78% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-cholestanol + O2
uncultured gamma proteobacterium Y-134
79% activity compared to cholesterol
?
-
-
?
1.1.3.6
beta-sitosterol + O2
Brevibacterium sterolicum
20% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
uncultured gamma proteobacterium
29% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptoverticillium cholesterolieum
39% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Corynebacterium cholesterolicum
50% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptomyces violascens
64% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Streptomyces sp.
72% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Chromobacterium sp.
80% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Burkholderia cepacia
84% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
Burkholderia cepacia ST-200
84% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-sitosterol + O2
uncultured gamma proteobacterium Y-134
29% activity compared to cholesterol
stigmast-5-en-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Brevibacterium sterolicum
10% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Corynebacterium cholesterolicum
12% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptoverticillium cholesterolieum
17% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
uncultured gamma proteobacterium
32% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Chromobacterium sp.
36% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptomyces sp.
37% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pseudomonas sp.
40% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pimelobacter simplex
47% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Streptomyces violascens
50% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Burkholderia cepacia
59% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
Pseudomonas sp. COX629
40% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
beta-stigmasterol + O2
uncultured gamma proteobacterium Y-134
32% activity compared to cholesterol
stigmasta-5,22-dien-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas aeruginosa
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Arthrobacter sp.
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces fradiae
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia rhodochrous
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia pseudomallei
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus hoagii
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces avermitilis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces griseus
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces natalensis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces sp.
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium urealyticum
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia farcinica
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium leprae
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium tuberculosis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces coelicolor
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus erythropolis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia thailandensis
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Paraburkholderia xenovorans
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pimelobacter simplex
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Schizophyllum commune
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces violascens
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium cholesterolicum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
uncultured gamma proteobacterium
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptomyces sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia cepacia
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Chromobacterium sp.
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Streptoverticillium cholesterolieum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Brevibacterium sterolicum
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Burkholderia cepacia ST-200
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Rhodococcus hoagii 103S
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Nocardia farcinica IFM 10152
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Pseudomonas sp. COX629
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
uncultured gamma proteobacterium Y-134
100% activity
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Corynebacterium urealyticum DSM 7109
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Mycobacterium tuberculosis H37Rv
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
cholesterol + O2
Arthrobacter sp. IM79
-
cholest-4-en-3-one + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Burkholderia cepacia
16% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptomyces sp.
24% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Chromobacterium sp.
27% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Schizophyllum commune
28% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptomyces violascens
37% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Brevibacterium sterolicum
41% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Streptoverticillium cholesterolieum
48% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Corynebacterium cholesterolicum
5% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
Burkholderia cepacia ST-200
16% activity compared to cholesterol
androst-5-en-3,17-dione + H2O2
-
-
?
1.1.3.6
epiandrosterone + O2
Nocardia sp.
1% activity compared to cholesterol
?
-
-
?
1.1.3.6
epiandrosterone + O2
Burkholderia cepacia
10% activity compared to cholesterol
?
-
-
?
1.1.3.6
epiandrosterone + O2
Chromobacterium sp.
15% activity compared to cholesterol
?
-
-
?
1.1.3.6
ergosterol + O2
Streptomyces sp.
12% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Chromobacterium sp.
12% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Streptomyces violascens
14% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pimelobacter simplex
17% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Burkholderia cepacia
20% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Corynebacterium cholesterolicum
4% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Streptoverticillium cholesterolieum
5% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pseudomonas sp.
51% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Burkholderia cepacia ST-200
20% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
ergosterol + O2
Pseudomonas sp. COX629
51% activity compared to cholesterol
dehydroergosterol + H2O2
-
-
?
1.1.3.6
additional information
Pseudomonas sp.
no activity with dehydroepiandrosterone
?
-
-
-
1.1.3.6
additional information
Schizophyllum commune
no activity with ergosterol
?
-
-
-
1.1.3.6
additional information
Brevibacterium sterolicum
no activity with ergosterol
?
-
-
-
1.1.3.6
additional information
uncultured gamma proteobacterium
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
?
-
-
-
1.1.3.6
additional information
Rhodococcus erythropolis
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
?
-
-
-
1.1.3.6
additional information
Pseudomonas sp. COX629
no activity with dehydroepiandrosterone
?
-
-
-
1.1.3.6
additional information
uncultured gamma proteobacterium Y-134
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
?
-
-
-
1.1.3.6
pregnenolone + O2
Schizophyllum commune
20% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Brevibacterium sterolicum
22% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Burkholderia cepacia
32% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptoverticillium cholesterolieum
37% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Corynebacterium cholesterolicum
38% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptomyces violascens
42% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Nocardia sp.
6% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Chromobacterium sp.
95% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Streptomyces sp.
99% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
1.1.3.6
pregnenolone + O2
Burkholderia cepacia ST-200
32% activity compared to cholesterol
5-pregnen-3,20-dione + H2O2
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.1.3.6
Acetone
the enzyme is unstable in 50% (v/v) acetone; the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Butanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
DMSO
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Emal 20CM
the enzyme is relatively tolerant to treatment with Emal 20CM even at 60°C
Chromobacterium sp.
1.1.3.6
Ethanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Ethyl acetate
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
isopropanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
Methanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Chromobacterium sp.
1.1.3.6
propan-2-ol
the activity of the enzyme is rapidly inactivated in the presence of 30% (v/v) propan-2-ol at 25°C
Brevibacterium sterolicum
1.1.3.6
SDS
the enzyme is relatively tolerant to treatment with SDS after incubation for 1 h at 30°C
Chromobacterium sp.
1.1.3.6
sodium cholate
the enzyme is stable at 50% (v/v) sodium cholate after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
sodium cholate
the enzyme is stable in sodium cholate after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
sodium cholate
the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C
uncultured gamma proteobacterium
1.1.3.6
sodium dodecyl sarcosinate
the enzyme is relatively tolerant to treatment with sodium dodecyl sarcosinate even at 60°C
Chromobacterium sp.
1.1.3.6
sodium laurylbenzenesulfonate
the enzyme is relatively tolerant to treatment with sodium laurylbenzenesulfonate after incubation for 1 h at 30°C
Chromobacterium sp.
1.1.3.6
sodium polyoxyethylene alkyl ether sulfate
the enzyme is stable at 50% (v/v) sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
sodium polyoxyethylene alkyl ether sulfate
the enzyme is stable in sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-100
the enzyme is stable at 50% (v/v) Triton X-100 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Triton X-100
the enzyme is stable in Triton X-100 after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-405
the enzyme is stable at 50% (v/v) Triton X-405 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Triton X-405
the enzyme is stable in Triton X-405 after incubation for 1 h at 30°C
Rhodococcus erythropolis
1.1.3.6
Triton X-405
the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C
uncultured gamma proteobacterium
1.1.3.6
Tween 20
the enzyme is stable at 50% (v/v) Tween 20 after incubation for 1 h at 30°C
Nocardia sp.
1.1.3.6
Tween 20
the enzyme is stable in Tween 20 after incubation for 1 h at 30°C
Rhodococcus erythropolis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.6
beta-cholestanol + O2
101% activity compared to cholesterol
710959
Streptomyces violascens
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
13% activity compared to cholesterol
710959
Brevibacterium sterolicum
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
69% activity compared to cholesterol
710959
Burkholderia cepacia
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
75% activity compared to cholesterol
710959
Streptomyces sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
78% activity compared to cholesterol
710959
Pseudomonas sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
79% activity compared to cholesterol
710959
uncultured gamma proteobacterium
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
95% activity compared to cholesterol
710959
Chromobacterium sp.
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
96% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
78% activity compared to cholesterol
710959
Pseudomonas sp. COX629
?
-
-
-
?
1.1.3.6
beta-cholestanol + O2
79% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
?
-
-
-
?
1.1.3.6
beta-sitosterol + O2
20% activity compared to cholesterol
710959
Brevibacterium sterolicum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
29% activity compared to cholesterol
710959
uncultured gamma proteobacterium
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
39% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
50% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
64% activity compared to cholesterol
710959
Streptomyces violascens
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
72% activity compared to cholesterol
710959
Streptomyces sp.
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
80% activity compared to cholesterol
710959
Chromobacterium sp.
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
84% activity compared to cholesterol
710959
Burkholderia cepacia
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
84% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-sitosterol + O2
29% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
stigmast-5-en-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
10% activity compared to cholesterol
710959
Brevibacterium sterolicum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
12% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
17% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
32% activity compared to cholesterol
710959
uncultured gamma proteobacterium
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
36% activity compared to cholesterol
710959
Chromobacterium sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
37% activity compared to cholesterol
710959
Streptomyces sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
40% activity compared to cholesterol
710959
Pseudomonas sp.
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
47% activity compared to cholesterol
710959
Pimelobacter simplex
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
50% activity compared to cholesterol
710959
Streptomyces violascens
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
59% activity compared to cholesterol
710959
Burkholderia cepacia
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
40% activity compared to cholesterol
710959
Pseudomonas sp. COX629
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
beta-stigmasterol + O2
32% activity compared to cholesterol
710959
uncultured gamma proteobacterium Y-134
stigmasta-5,22-dien-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Pseudomonas aeruginosa
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Arthrobacter sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces fradiae
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia rhodochrous
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Burkholderia pseudomallei
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus hoagii
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces avermitilis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces griseus
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces natalensis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Corynebacterium urealyticum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia farcinica
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium leprae
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium tuberculosis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Streptomyces coelicolor
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus erythropolis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Burkholderia thailandensis
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Paraburkholderia xenovorans
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pseudomonas sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pimelobacter simplex
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Nocardia sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Schizophyllum commune
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptomyces violascens
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Corynebacterium cholesterolicum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
uncultured gamma proteobacterium
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptomyces sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Burkholderia cepacia
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Chromobacterium sp.
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Streptoverticillium cholesterolieum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Brevibacterium sterolicum
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Burkholderia cepacia ST-200
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Rhodococcus hoagii 103S
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Nocardia farcinica IFM 10152
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
Pseudomonas sp. COX629
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
100% activity
710959
uncultured gamma proteobacterium Y-134
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Corynebacterium urealyticum DSM 7109
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Mycobacterium tuberculosis H37Rv
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
cholesterol + O2
-
710959
Arthrobacter sp. IM79
cholest-4-en-3-one + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
16% activity compared to cholesterol
710959
Burkholderia cepacia
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
24% activity compared to cholesterol
710959
Streptomyces sp.
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
27% activity compared to cholesterol
710959
Chromobacterium sp.
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
28% activity compared to cholesterol
710959
Schizophyllum commune
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
37% activity compared to cholesterol
710959
Streptomyces violascens
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
41% activity compared to cholesterol
710959
Brevibacterium sterolicum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
48% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
5% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
dehydroepiandrosterone + O2
16% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
androst-5-en-3,17-dione + H2O2
-
-
-
?
1.1.3.6
epiandrosterone + O2
1% activity compared to cholesterol
710959
Nocardia sp.
?
-
-
-
?
1.1.3.6
epiandrosterone + O2
10% activity compared to cholesterol
710959
Burkholderia cepacia
?
-
-
-
?
1.1.3.6
epiandrosterone + O2
15% activity compared to cholesterol
710959
Chromobacterium sp.
?
-
-
-
?
1.1.3.6
ergosterol + O2
12% activity compared to cholesterol
710959
Streptomyces sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
12% activity compared to cholesterol
710959
Chromobacterium sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
14% activity compared to cholesterol
710959
Streptomyces violascens
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
17% activity compared to cholesterol
710959
Pimelobacter simplex
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
20% activity compared to cholesterol
710959
Burkholderia cepacia
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
4% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
5% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
51% activity compared to cholesterol
710959
Pseudomonas sp.
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
20% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
ergosterol + O2
51% activity compared to cholesterol
710959
Pseudomonas sp. COX629
dehydroergosterol + H2O2
-
-
-
?
1.1.3.6
additional information
no activity with dehydroepiandrosterone
710959
Pseudomonas sp.
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol
710959
Schizophyllum commune
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol
710959
Brevibacterium sterolicum
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
710959
uncultured gamma proteobacterium
?
-
-
-
-
1.1.3.6
additional information
the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol
710959
Rhodococcus erythropolis
?
-
-
-
-
1.1.3.6
additional information
no activity with dehydroepiandrosterone
710959
Pseudomonas sp. COX629
?
-
-
-
-
1.1.3.6
additional information
no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone
710959
uncultured gamma proteobacterium Y-134
?
-
-
-
-
1.1.3.6
pregnenolone + O2
20% activity compared to cholesterol
710959
Schizophyllum commune
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
22% activity compared to cholesterol
710959
Brevibacterium sterolicum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
32% activity compared to cholesterol
710959
Burkholderia cepacia
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
37% activity compared to cholesterol
710959
Streptoverticillium cholesterolieum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
38% activity compared to cholesterol
710959
Corynebacterium cholesterolicum
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
42% activity compared to cholesterol
710959
Streptomyces violascens
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
6% activity compared to cholesterol
710959
Nocardia sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
95% activity compared to cholesterol
710959
Chromobacterium sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
99% activity compared to cholesterol
710959
Streptomyces sp.
5-pregnen-3,20-dione + H2O2
-
-
-
?
1.1.3.6
pregnenolone + O2
32% activity compared to cholesterol
710959
Burkholderia cepacia ST-200
5-pregnen-3,20-dione + H2O2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.6
30
-
-
Nocardia rhodochrous
1.1.3.6
40
42
-
Corynebacterium cholesterolicum
1.1.3.6
40
50
-
Arthrobacter sp.
1.1.3.6
40
-
-
Rhodococcus hoagii
1.1.3.6
45
50
-
Streptomyces sp.
1.1.3.6
50
-
-
Brevibacterium sterolicum
1.1.3.6
50
-
-
Pimelobacter simplex
1.1.3.6
50
-
-
Streptomyces violascens
1.1.3.6
60
-
-
Burkholderia cepacia
1.1.3.6
65
-
-
Chromobacterium sp.
1.1.3.6
70
-
-
Streptomyces fradiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.6
50
60
96% activity after 30 min at 50°C, 6% activity after 30 min at 60°C
Corynebacterium cholesterolicum
1.1.3.6
55
-
less than 50% activity after 15 min at 55°C
Streptomyces sp.
1.1.3.6
60
85
the enzyme retains more than 90% of its original activity after incubation for 240 min at 60°C, 90% activity after 1 h at 80°C, and 80% activity after 30 min at 85°C
Chromobacterium sp.
1.1.3.6
70
80
70% residual activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C
Burkholderia cepacia
1.1.3.6
70
80
70% activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C
Pseudomonas sp.
1.1.3.6
70
-
10% activity after 1 h at 70°C
Streptomyces fradiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.6
5
-
-
Schizophyllum commune
1.1.3.6
6.5
7
-
Streptomyces sp.
1.1.3.6
6.5
-
-
uncultured gamma proteobacterium
1.1.3.6
6.8
8
-
Burkholderia cepacia
1.1.3.6
7
7.5
-
Chromobacterium sp.
1.1.3.6
7
7.5
-
Corynebacterium cholesterolicum
1.1.3.6
7
7.5
-
Streptoverticillium cholesterolieum
1.1.3.6
7
-
-
Nocardia rhodochrous
1.1.3.6
7
-
-
Nocardia sp.
1.1.3.6
7
-
-
Pseudomonas sp.
1.1.3.6
7
-
-
Streptomyces fradiae
1.1.3.6
7
-
-
Streptomyces sp.
1.1.3.6
7
-
-
Streptomyces violascens
1.1.3.6
7.5
8.5
-
Arthrobacter sp.
1.1.3.6
7.5
-
-
Brevibacterium sterolicum
1.1.3.6
7.5
-
-
Pimelobacter simplex
1.1.3.6
8
-
-
Rhodococcus hoagii
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.6
3
11
30°C, 1 h
Chromobacterium sp.
1.1.3.6
4
10
37°C, 30 min
Brevibacterium sterolicum
1.1.3.6
4
10
4°C, 4 h
Streptomyces fradiae
1.1.3.6
4
11
30°C, 1 h
Burkholderia cepacia
1.1.3.6
4
11
60°C, 1 min
Pseudomonas sp.
1.1.3.6
4
12.5
22°C, 1 h
Streptoverticillium cholesterolieum
1.1.3.6
4
8
25°C, 20 h
Streptomyces sp.
1.1.3.6
5
7.5
60°C, 1 h
uncultured gamma proteobacterium
1.1.3.6
5
9
30°C, 1 h
Corynebacterium cholesterolicum
General Information
EC Number
General Information
Commentary
Organism
1.1.3.6
metabolism
PimE is a key enzyme in the biosynthesis of the polyene macrolide pimaricin
Streptomyces natalensis
1.1.3.6
physiological function
ChoD plays a significant role in the pathogenesis of Mycobacterium tuberculosis
Mycobacterium tuberculosis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.3.6
metabolism
PimE is a key enzyme in the biosynthesis of the polyene macrolide pimaricin
Streptomyces natalensis
1.1.3.6
physiological function
ChoD plays a significant role in the pathogenesis of Mycobacterium tuberculosis
Mycobacterium tuberculosis