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Literature summary extracted from

  • Vargas, W.A.; Weyman, P.D.; Tong, Y.; Smith, H.O.; Xu, Q.
    A [NiFe]-hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature (2011), Appl. Environ. Microbiol., 77, 1990-1998.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytoplasm 70% of total activity Alteromonas macleodii 5737
-
1.12.99.6 membrane losely membrane-bound, 30% of total activity Alteromonas macleodii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe2+ essential element for the assembly and maturation, the addition of Fe-EDTA (0.05 mM) does not affect the level of hydrogenase activity Alteromonas macleodii
1.12.99.6 additional information no increase in the hydrogenase activity is observed in response to ferric sulfate, ferric citrate, ferric ammonium citrate, and ferric nitrate Alteromonas macleodii
1.12.99.6 Ni2+ essential element for the assembly and maturation, addition of 0.1 mM Ni2+ to the growth medium significantly enhances the hydrogenase activity. Nickel-treatment affects the level of the protein, but not the mRNA Alteromonas macleodii

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Alteromonas macleodii
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.99.6 after exposure to air for 45 days at 4°C, the enzyme retains nearly 100% of its activity when assayed under anaerobic conditions. In 1% O2, 20.4% of its hydrogen-deuterium exchange activity is retained. The accumulation of active HynSL enzyme is enhanced in the presence of molecular oxygen. The activity drops to 5.2% when the reaction is exposed to 3% O2 and no activity is detected in 5% O2 Alteromonas macleodii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 DEAE 52 cellulose column chromatography Alteromonas macleodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + benzyl viologen
-
 Alteromonas macleodii H+ + reduced benzyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 HynSL
-
 Alteromonas macleodii
1.12.99.6 [NiFe]-hydrogenase
-
 Alteromonas macleodii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 70 80 the partially purified enzyme retains 94% and 84% of its activity after exposure to 70°C and 80°C for 2 h, respectively Alteromonas macleodii