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Literature summary extracted from

  • Ullrich, R.; Nuske, J.; Scheibner, K.; Spantzel, J.; Hofrichter, M.
    Novel haloperoxidase from the agaric basidiomycete Agrocybe aegerita oxidizes aryl alcohols and aldehydes (2004), Appl. Environ. Microbiol., 70, 4575-4581.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.2.1 H2O2 in the presence of 5 mM veratryl alcohol, optimal activity is observed with 2 mM H2O2, but the enzyme still exhibits 35% of the maximum activity with 10 mM H2O2 Cyclocybe aegerita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.037
-
 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita
1.11.2.1 0.298
-
 2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 1.001
-
 benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 1.313
-
 H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 2.367
-
 veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.1 46000
-
 isozymes P I and P II, SDS-PAGE Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
 strain TMA1, synonym of Agrocybe cylindracea, black poplar mushroom
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1 Q Sepharose column chromatography, SP Sepharose column chromatography, and Mono S column chromatography Cyclocybe aegerita

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 3
-
 peroxidase from culture liquid, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 71.8
-
 purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Cl-) as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 99.6
-
 purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using 2,6-dimethoxyphenol as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 165
-
 peroxidase isozyme P II, after 55fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 167
-
 peroxidase isozyme P I, after 56fold purification, at pH 7.0 in sodium citrate/phosphate buffer, using veratryl alcohol as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 234.2
-
 purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzyl alcohol as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 236.7
-
 purified peroxidase, at pH 7.0 in sodium citrate/phosphate buffer, using benzaldehyde as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 295.7
-
 purified peroxidase, at pH 5.0 in sodium citrate/phosphate buffer, using 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) as substrate, at 25°C Cyclocybe aegerita
1.11.2.1 354.3
-
 purified peroxidase, at pH 2.75 in phosphate buffer, using monochlorodimedone (Br-) as substrate, at 25°C Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2
-
 Cyclocybe aegerita oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O
-
 ?
1.11.2.1 2,6-dimethoxyphenol + H2O2
-
 Cyclocybe aegerita oxidized 2,6-dimethoxyphenol + H2O
-
 ?
1.11.2.1 anisaldehyde + H2O2
-
 Cyclocybe aegerita ? + H2O
-
 ?
1.11.2.1 anisyl alcohol + H2O2
-
 Cyclocybe aegerita anisaldehyde + H2O
-
 ?
1.11.2.1 benzaldehyde + H2O2
-
 Cyclocybe aegerita benzoic acid + H2O
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
1.11.2.1 additional information the enzyme exhibits also haloperoxidase activity as shown by the chlorination or bromination of monochlorodimedone. Ethanol is not oxidized by the peroxidase Cyclocybe aegerita ?
-
 ?
1.11.2.1 veratraldehyde + H2O2
-
 Cyclocybe aegerita veratric acid + H2O
-
 ?
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 85
-
 veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 108
-
 2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 269
-
 benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 283
-
 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita
1.11.2.1 367
-
 H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 4.7
-
 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) oxidation occurs in a narrow pH range (pH 2.0-6.5), with an acidic maximum around pH 4.7 Cyclocybe aegerita
1.11.2.1 5.5
-
 the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 5.5 and 7.0) Cyclocybe aegerita
1.11.2.1 6.2
-
 the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita
1.11.2.1 7
-
 the enzyme has two pH optima for the oxidation of veratryl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita
1.11.2.1 7
-
 the optimum pH for the oxidation of aryl alcohols is around 7.0 Cyclocybe aegerita
1.11.2.1 7.2
-
 the enzyme has two pH optima for the oxidation of benzyl alcohol (pH 6.2 and 7.2) Cyclocybe aegerita

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.2.1 2.5 9
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.2.1 Cyclocybe aegerita isozyme P I, isoelectric focusing 5.4 4.6
1.11.2.1 Cyclocybe aegerita isozyme P II, isoelectric focusing 5.6 4.9

Expression

EC Number Organism Comment Expression
1.11.2.1 Cyclocybe aegerita highest enzyme levels are detected in the presence of soybean meal (60 g/l) up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 35.8
-
 veratryl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 269
-
 benzyl alcohol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 279
-
 H2O2 at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 361
-
 2,6-dimethoxyphenol at pH 7.0 in sodium phosphate/citrate buffer, at 25°C Cyclocybe aegerita
1.11.2.1 7670
-
 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at pH 4.5 in sodium phosphate/citrate buffer, in the presence of 5 mM benzyl alcohol, at 25°C Cyclocybe aegerita