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Literature summary extracted from

  • Saxena, U.; Goswami, P.
    Silk mat as bio-matrix for the immobilization of cholesterol oxidase (2010), Appl. Biochem. Biotechnol., 162, 1122-1131.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.6 the ChOx-immobilized silk mat maintains its initial activity till the fourth successive measurement and retains about 50% of its initial activity at the end of 25 measurements Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.2123
-
 cholesterol apparent value, free enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C Pseudomonas fluorescens
1.1.3.6 0.2599
-
 cholesterol apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pseudomonas fluorescens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.1.3.6 4°C, ChOx-immobilized silk mat, when the measurements are made at 3 days interval the immobilized ChOx can retain about 50% of its original activity after 45 days of storage and when the same carried out with 7 days interval, about 70% of its original activity is retained till 60 days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Pseudomonas fluorescens cholest-4-en-3-one + H2O2
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 ChOx
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 30
-
 silk mat-immobilized enzyme Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 25 45 the oxygen depletion rate is increased with increasing temperature from 25°C to 40°C but it decreases sharply as the temperature increases further to 45°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7.5
-
 silk mat-immobilized enzyme Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 4 7.5 the activity increases with increasing pH value from 4.0 to 7.5 and then declines as pH increases further Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD
-
 Pseudomonas fluorescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.6 0.0013
-
 cholesterol apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C Pseudomonas fluorescens