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Literature summary extracted from

  • Fyfe, P.; Rao, V.; Zemla, A.; Cameron, S.; Hunter, W.
    Specificity and mechanism of Acinetobacter baumanii nicotinamidase: Implications for activation of the front-line tuberculosis drug pyrazinamide (2009), Angew. Chem. Int. Ed. Engl., 48, 9176-9179.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.B15 Fe2+ activates Acinetobacter baumannii
3.5.1.B15 Mn2+ activates Acinetobacter baumannii
3.5.1.B15 additional information PncA is a divalent cation-dependent enzyme Acinetobacter baumannii
3.5.1.B15 Zn2+ activates, best cation, the active site Cys159 is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site Acinetobacter baumannii
3.5.1.19 Fe2+ activates Acinetobacter baumannii
3.5.1.19 Mn2+ activates Acinetobacter baumannii
3.5.1.19 additional information PncA is a divalent cation-dependent enzyme Acinetobacter baumannii
3.5.1.19 Zn2+ activates, best cation, the active site Cys159 is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site Acinetobacter baumannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.B15 47000
-
 gel filtratrion and analytical ultracentrifugation Acinetobacter baumannii
3.5.1.19 47000
-
 gel filtratrion and analytical ultracentrifugation Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.B15 pyrazinamide + H2O Acinetobacter baumannii a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid pyrazinoic acid + NH3 a bacteriostatic drug ?
3.5.1.19 nicotinamide + H2O Acinetobacter baumannii
-
 nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O Acinetobacter baumannii a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA pyrazinoic acid + NH3 a bacteriostatic drug ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.B15 Acinetobacter baumannii
-
-
-
3.5.1.19 Acinetobacter baumannii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.19 nicotinamide + H2O = nicotinate + NH3 the active site Cys159, conserved and essential, is located at the N-terminus of a6 at one side of the active site with the Zn2+ ion positioned on the other side. The metal ion is bound tightly in the AbPncA active site. The reaction proceeds in a four-stage mechanism via a tetrahedral and an acyl intermediate, overview Acinetobacter baumannii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.B15 pyrazinamide + H2O a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid Acinetobacter baumannii pyrazinoic acid + NH3 a bacteriostatic drug ?
3.5.1.19 nicotinamide + H2O
-
 Acinetobacter baumannii nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O active site structure, overview Acinetobacter baumannii nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O a nicotinamide prodrug. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA Acinetobacter baumannii pyrazinoic acid + NH3 a bacteriostatic drug ?

Subunits

EC Number Subunits Comment Organism
3.5.1.B15 dimer
-
 Acinetobacter baumannii
3.5.1.19 dimer
-
 Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.B15 PncA
-
 Acinetobacter baumannii
3.5.1.B15 pyrazinamidase
-
 Acinetobacter baumannii
3.5.1.19 PncA
-
 Acinetobacter baumannii
3.5.1.19 pyrazinamidase
-
 Acinetobacter baumannii

General Information

EC Number General Information Comment Organism
3.5.1.B15 metabolism in Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid Acinetobacter baumannii
3.5.1.B15 physiological function the reaction product pyrazinoic acid inhibits Mycobacterium tuberculosis type I fatty acid synthase, represses mycolic acid biosynthesis, and appears to affect membrane energetics and acidification of the cytoplasm Acinetobacter baumannii
3.5.1.19 metabolism the enzyme catalyzes the deamination of nicotinamide, which is an important step in the NAD+ salvage pathway. In Mycobacterium tuberculosis the enzyme converts the nicotinamide analogue prodrug pyrazinamide into the bacteriostatic pyrazinoic acid, hence the alternative name, pyrazinamidase, PncA Acinetobacter baumannii
3.5.1.19 physiological function the reaction product pyrazinoic acid inhibits Mycobacterium tuberculosis type I fatty acid synthase, represses mycolic acid biosynthesis, and appears to affect membrane energetics and acidification of the cytoplasm Acinetobacter baumannii