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Literature summary extracted from

  • Kumar, P.; Gomez-Gil, L.; Mohammadi, M.; Sylvestre, M.; Eltis, L.D.; Bolin, J.T.
    Anaerobic crystallization and initial X-ray diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals (2011), Acta Crystallogr. Sect. F, 67, 59-62.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 expressed in Escherichia coli C41 (DE3) cells Paraburkholderia xenovorans
1.14.12.18 expression of His-tagged subunits alpha and beta and mutant variants in Escherichia coli strain C41 (DE3) Paraburkholderia xenovorans
1.14.12.18 expression of His-tagged subunits alpha and beta and mutant variants in Escherichia coli strain C41(DE3) Paraburkholderia xenovorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.18 purified recombinant BPDOLB400, sitting drop vapour diffusion method, anaerobic conditions, method optimization, 0.002 ml of 8 mg/ml protein in 25 mM HEPES, pH 7.3, containing 0.25 mM ferrous ammonium sulfate, and 2 mM dithiothreitol, is mixed with 0.002 ml of reservoir solution containing 20-25% w/v PEG 8000 or PEG 5000 MME, 50 mM PIPES, pH 6.5, 100 mM ammonium acetate, 5% v/v glycerol, and 0.2% w/v agarose, successful crystallization only occurs at pH 6.5, X-ray diffraction structure determination and analysis at 2.4-2.8 A resolution Paraburkholderia xenovorans
1.14.12.18 sitting drop vapor diffusion method, using 20-25% (w/v) PEG 8000 or PEG 5000 MME, 50 mM PIPES pH 6.5, 100 mM ammonium acetate, 5% (v/v) glycerol and 0.2% (w/v) agarose Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2 Paraburkholderia xenovorans
-
 2,3-dihydro-dihydroxybiphenyl + NAD+
-
 ?
1.14.12.18 biphenyl + NADH + H+ + O2 Paraburkholderia xenovorans
-
 (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Paraburkholderia xenovorans
-
-
-
1.14.12.18 Paraburkholderia xenovorans P37333 subunit alpha
-
1.14.12.18 Paraburkholderia xenovorans P37334 subunit beta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18
-
 Paraburkholderia xenovorans
1.14.12.18 recombinant His-tagged subunits alpha and beta and mutant variants from Escherichia coli strain C41 (DE3) by affinity chromatography, the tag is cleaved off by thrombin Paraburkholderia xenovorans
1.14.12.18 recombinant His-tagged subunits alpha and beta and mutant variants from Escherichia coli strain C41(DE3) by affinity chromatography, the tag is cleaved off by thrombin Paraburkholderia xenovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 2,3-dihydro-dihydroxybiphenyl + NAD+
-
 ?
1.14.12.18 biphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 BPDO
-
 Paraburkholderia xenovorans
1.14.12.18 BPDOLB400
-
 Paraburkholderia xenovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Paraburkholderia xenovorans

General Information

EC Number General Information Comment Organism
1.14.12.18 metabolism biphenyl 2,3-dioxygenase catalyzes the initial step in the degradation of biphenyl and some polychlorinated biphenyls Paraburkholderia xenovorans