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Literature summary extracted from

  • Ismaya, W.T.; Rozeboom, H.J.; Schurink, M.; Boeriu, C.G.; Wichers, H.; Dijkstra, B.W.
    Crystallization and preliminary X-ray crystallographic analysis of tyrosinase from the mushroom Agaricus bisporus (2011), Acta Crystallogr. Sect. F, 67, 575-578.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.1 hanging drop vapor diffusion method, using 10% (w/v) PEG 4000, 100 mM sodium acetate buffer pH 4.6 and 5 mM HoCl3 Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 14000
-
 2 * 45000 + 2 * 14000, SDS-PAGE Agaricus bisporus
1.14.18.1 45000
-
 2 * 45000 + 2 * 14000, SDS-PAGE Agaricus bisporus
1.14.18.1 110000
-
 dynamic light scattering Agaricus bisporus
1.14.18.1 120000
-
 gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 L-tyrosine + O2 Agaricus bisporus
-
 dihydroxyphenylalanine quinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus O42713
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 Superdex S-200 filtration Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-Dopa + O2
-
 Agaricus bisporus L-dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2
-
 Agaricus bisporus dihydroxyphenylalanine quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 heterotetramer 2 * 45000 + 2 * 14000, SDS-PAGE Agaricus bisporus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyrosinase
-
 Agaricus bisporus