Literature summary extracted from
Higashi, Y.; Smith, T.J.; Jez, J.M.; Kutchan, T.M.
Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum (2010), Acta Crystallogr. Sect. F, 66, 163-166.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.248 |
expression in Escherichia coli |
Papaver somniferum |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.1.1.248 |
to 1.9 A resolution, space group P6422 or P6222. Presence of NADPH is required for crystallization |
Papaver somniferum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.248 |
Papaver somniferum |
Q071N0 |
- |
- |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.1.1.248 |
storage of purified at 193 K without 2-mercaptoethanol results in complete loss of activity. Inactive enzyme regains activity on incubation overnight at 277 K with 2-mercaptoethanol |
Papaver somniferum |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.1.1.248 |
SalR is susceptible to inactivation and soluble aggregation in an oxidative environment |
Papaver somniferum |
1.1.1.248 |
storage of purified at 193 K without 2-mercaptoethanol results in complete loss of activity. Inactive enzyme regains activity on incubation overnight at 277 K with 2-mercaptoethanol |
Papaver somniferum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.248 |
SalR |
- |
Papaver somniferum |