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Literature summary extracted from

  • Axelrod, H.L.; Das, D.; Abdubek, P.; Astakhova, T.; Bakolitsa, C.; Carlton, D.; Chen, C.; Chiu, H.J.; Clayton, T.; Deller, M.C.; et al
    Structures of three members of Pfam PF02663 (FmdE) implicated in microbial methanogenesis reveal a conserved alpha+beta core domain and an auxiliary C-terminal treble-clef zinc finger (2010), Acta Crystallogr. Sect. F, 66, 1335-1346.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.12 expressed in Escherichia coli Thermoplasma acidophilum
1.2.7.12 expressed in Escherichia coli Desulfitobacterium hafniense
1.2.7.12 expressed in Escherichia coli Syntrophus aciditrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.12 nanodroplet vapor diffusion method, using 0.01 M nickel chloride, 20% (w/v) PEG MME 2000 and 0.1 M Tris pH 8.5 Syntrophus aciditrophicus
1.2.7.12 nanodroplet vapor diffusion method, using 0.2 M magnesium nitrate and 20% (w/v) PEG 3350 or 10% (w/v) PEG 8000, 0.2 M zinc acetate and 0.1 M MES pH 6.0 Thermoplasma acidophilum
1.2.7.12 nanodroplet vapor diffusion method, using 0.2 M MgCl2 and 20% (w/v) PEG 3350 Desulfitobacterium hafniense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.12 Ni2+ contains nickel Desulfitobacterium hafniense
1.2.7.12 Zn2+ contains zinc Thermoplasma acidophilum
1.2.7.12 Zn2+ contains zinc Desulfitobacterium hafniense
1.2.7.12 Zn2+ contains zinc Syntrophus aciditrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.12 17400
-
 calculated from amino acid sequence Desulfitobacterium hafniense
1.2.7.12 21500
-
 calculated from amino acid sequence Syntrophus aciditrophicus
1.2.7.12 23100
-
 calculated from amino acid sequence Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.12 Desulfitobacterium hafniense B8FYU2 subunit E
-
1.2.7.12 Desulfitobacterium hafniense DCB-2 B8FYU2 subunit E
-
1.2.7.12 Syntrophus aciditrophicus Q2LQ23 subunit E
-
1.2.7.12 Syntrophus aciditrophicus SB Q2LQ23 subunit E
-
1.2.7.12 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.12 nickel-chelating resin column chromatography Thermoplasma acidophilum
1.2.7.12 nickel-chelating resin column chromatography Desulfitobacterium hafniense
1.2.7.12 nickel-chelating resin column chromatography Syntrophus aciditrophicus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.12 FmdE
-
 Thermoplasma acidophilum
1.2.7.12 FmdE
-
 Desulfitobacterium hafniense
1.2.7.12 FmdE
-
 Syntrophus aciditrophicus
1.2.7.12 PF02663
-
 Thermoplasma acidophilum
1.2.7.12 PF02663
-
 Desulfitobacterium hafniense
1.2.7.12 PF02663
-
 Syntrophus aciditrophicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.7.12 Desulfitobacterium hafniense calculated from amino acid sequence
-
 5.6
1.2.7.12 Thermoplasma acidophilum calculated from amino acid sequence
-
 6.1
1.2.7.12 Syntrophus aciditrophicus calculated from amino acid sequence
-
 6.2