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Literature summary extracted from

  • Maeda, Y.; Doubayashi, D.; Ootake, T.; Oki, M.; Mikami, B.; Uchida, H.
    Crystallization and preliminary X-ray analysis of formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family (2010), Acta Crystallogr. Sect. F, 66, 1064-1066.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.1 expressed in Escherichia coli BL21(DE3) cells Aspergillus oryzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.3.1 hanging drop vapor diffusion method, using 0.1 M sodium acetate buffer pH 4.6 and 6% (w/v) PEG 4000, at 20°C Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Aspergillus oryzae
-
-
-
1.2.3.1 Aspergillus oryzae RIB 40
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.1 Ni Sepharose 6 Fast Flow column chromatography, HiTrap QHP column chromatography, and HisTrap FF column chromatography Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 formate + O2
-
 Aspergillus oryzae CO2 + H2O2
-
 ?
1.2.3.1 formate + O2
-
 Aspergillus oryzae RIB 40 CO2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.1 trimer x-ray crystallography Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 FOD
-
 Aspergillus oryzae
1.2.3.1 formate oxidase
-
 Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.3.1 FAD
-
 Aspergillus oryzae