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Literature summary extracted from

  • Homma, K.; Moriyama, H.
    Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase (2009), Acta Crystallogr. Sect. F, 65, 1030-1034.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 hanging drop vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.65 for the His-tagged enzyme, using 11% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.65 for the His-tagged enzyme in complex with dUTP, or using 10% (w/v) polyethylene glycol 4000, 10% (w/v) 2-propanol and 0.3 M sodium citrate pH 5.6 for the mutant enzyme E81S/T84R in complex with dUTP Chlorella virus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 E81S/T84R the mutations lead to an increase in the optimal temperature of the enzyme to 55°C Chlorella virus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Chlorella virus
-
-
-
3.6.1.23 Chlorella virus IL-3A
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Chlorella virus dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Chlorella virus IL-3A dUMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine triphosphatase
-
 Chlorella virus
3.6.1.23 dUTPase
-
 Chlorella virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 37
-
 wild type enzyme Chlorella virus
3.6.1.23 55
-
 mutant enzyme E81S/T84R Chlorella virus