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Literature summary extracted from

  • Malik, R.; Viola, R.E.
    Structural characterization of tartrate dehydrogenase: a versatile enzyme catalyzing multiple reactions (2010), Acta Crystallogr. Sect. D, 66, 673-684.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.93 expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.93 in complex with the intermediate analog oxalate, Mg2+ and NADH, to 2.0 A resolution Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.93 D225A mutation in metal ion-binding ligand, 20fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis Pseudomonas putida
1.1.1.93 D250A mutation in metal ion-binding ligand, 10fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis Pseudomonas putida
1.1.1.93 R108L 1.3% of wild-type catalytic rate Pseudomonas putida
1.1.1.93 R108Q 8.7% of wild-type catalytic rate Pseudomonas putida
1.1.1.93 R98L mutant is not expressed in stable form Pseudomonas putida
1.1.1.93 R98Q 2.6% of wild-type catalytic rate Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.93 0.025
-
 NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.079
-
 D-malate wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.093
-
 D-malate mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.096
-
 NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.098
-
 D-malate mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.135
-
 NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.18
-
 D-malate mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.24
-
 NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.93 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.93 recombinant enzyme Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.93 tartrate + NAD+ = oxaloglycolate + NADH + H+ lysyl amino group, Lys192 is the base responsible for the water-mediated proton abstraction from the C2 hydroxyl group of the substrate that begins the catalytic reaction, followed by hydride transfer to NAD. The hydroxyl group of Tyr141 functions as a general acid to protonate the enolate intermediate. Each substrate undergoes the initial hydride transfer Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.93 D-malate + NAD+
-
 Pseudomonas putida pyruvate + CO2 + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.93 0.015
-
 NAD+ mutant D225A, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.06
-
 NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.087
-
 NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.088
-
 NAD+ mutant D250A, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.175
-
 NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 6.5
-
 NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.93 0.36
-
 NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 0.48
-
 D-malate mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 1.8
-
 NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 1.8
-
 D-malate mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 4.3
-
 NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 6.2
-
 D-malate mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 82
-
 D-malate wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.1.1.93 260
-
 NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida