EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.1 | Cu2+ | phenoloxdiase is a copper-containing metalloenzyme | Amphioctopus fangsiao |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.1 | 1-Phenyl-2-thiourea | complete inhibition at 5 mM | Amphioctopus fangsiao | |
1.10.3.1 | ascorbic acid | 70.95% inhibition at 20 mM | Amphioctopus fangsiao | |
1.10.3.1 | benzoic acid | 64.86% inhibition at 10 mM | Amphioctopus fangsiao | |
1.10.3.1 | Citric acid | 68.92% inhibition at 20 mM | Amphioctopus fangsiao | |
1.10.3.1 | Cu2+ | 91.45% inhibition at 20 mM | Amphioctopus fangsiao | |
1.10.3.1 | diethyldithiocarbamate | 72.37% inhibition at 20 mM, the diethyldithiocarbamate-inhibited phenoloxidase-like activity can be perfectly restored by 10 mM Cu2+ while Zn2+ has no recovery effect | Amphioctopus fangsiao | |
1.10.3.1 | EDTA | 75% inhibition at 10 mM | Amphioctopus fangsiao | |
1.10.3.1 | L-cysteine | 43.24% inhibition at 20 mM | Amphioctopus fangsiao | |
1.10.3.1 | additional information | no inhibition by Ca2+ and Mg2+ | Amphioctopus fangsiao | |
1.10.3.1 | sodium sulfite | complete inhibition at 10 mM | Amphioctopus fangsiao | |
1.10.3.1 | Thiourea | 84.46% inhibition at 10 mM | Amphioctopus fangsiao | |
1.10.3.1 | Zn2+ | 45.39% inhibition at 20 mM | Amphioctopus fangsiao |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.1 | 3.1 | - |
L-Dopa | in 20 mM Tris-HCl buffer at pH 7.1 and 28°C | Amphioctopus fangsiao | |
1.10.3.1 | 6.3 | - |
catechol | in 20 mM Tris-HCl buffer at pH 7.1 and 28°C | Amphioctopus fangsiao |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 73000 | - |
1 * 75600 + 1 * 73000, proenzyme, estimated from SDS-PAGE | Amphioctopus fangsiao |
1.10.3.1 | 75600 | - |
1 * 75600 + 1 * 73000, proenzyme, estimated from SDS-PAGE | Amphioctopus fangsiao |
1.10.3.1 | 153800 | - |
gel filtration | Amphioctopus fangsiao |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.1 | Amphioctopus fangsiao | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.1 | ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sephacryl S-100 gel filtration | Amphioctopus fangsiao |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.10.3.1 | ink sac | - |
Amphioctopus fangsiao | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | additional information | - |
the specific activity of the crude enzyme is 3.72 units/mg, the specific activity of the 28.24fold purified enzyme is 105 units/mg, one unit is defined as an increase in the rate of absorbance of 0.001 per min, using L-dopa as substrate, at pH 7.1 and 28°C | Amphioctopus fangsiao |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.1 | 2 catechol + O2 | - |
Amphioctopus fangsiao | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.10.3.1 | L-Dopa + O2 | specific substrate | Amphioctopus fangsiao | L-dopaquinone + H2O | - |
? | |
1.10.3.1 | additional information | the enzyme fails to oxidize hydroquinone, phenol, or tyrosine | Amphioctopus fangsiao | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.1 | heterodimer | 1 * 75600 + 1 * 73000, proenzyme, estimated from SDS-PAGE | Amphioctopus fangsiao |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.1 | phenoloxidase | - |
Amphioctopus fangsiao |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 40 | - |
- |
Amphioctopus fangsiao |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 30 | 50 | - |
Amphioctopus fangsiao |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 7 | - |
- |
Amphioctopus fangsiao |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.1 | 6 | 8 | - |
Amphioctopus fangsiao |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.10.3.1 | physiological function | phenoloxidase functions as a catalytic enzyme in melanin production in ink sacs and also as a humoral factor in host defense via melaninization | Amphioctopus fangsiao |