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Literature summary extracted from
- Atan1p-an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme (2009), Yeast, 26, 323-337.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | recombinant ATAN1 gene overexpressed in the auxotrophic mutant strain Arxula adeninivorans G1212 [DELTAatrp1] under the control of the strong, constitutive TEF1 promoter. Expression module assembled in the plasmid pGH101-TEF1-ATAN1 and used to construct YIEC101-TEF1-ATAN1 for transformation of Arxula host cells | Blastobotrys adeninivorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.20 | Cd2+ | 45% inhibition, at 30°C in sodium citrate buffer, pH 6.0 | Blastobotrys adeninivorans |  |
| 3.1.1.20 | Cu2+ | 51% inhibition, at 30°C in sodium citrate buffer, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | Fe3+ | 35% inhibition, at 30°C in sodium citrate buffer, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | gallic acid | competitive inhibition using gallic acid as substrate | Blastobotrys adeninivorans | |
| 3.1.1.20 | additional information | EDTA, Mg2+, Mn2+, Ca2+, Zn2+ and EDTA have no or only weak effects | Blastobotrys adeninivorans | |
| 3.1.1.20 | phenyl methyl sulphonyl fluoride | 95% inhibition, at 30°C in sodium citrate buffer, pH 6.0 | Blastobotrys adeninivorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.14 | - |
Tannic acid | endogenous tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 0.17 | - |
Tannic acid | recombinant tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 3.5 | - |
methyl gallate | recombinant tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 4.4 | - |
methyl gallate | endogenous tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 55000 | - |
4 * 80000, denatured endogenous and recombinant tannases, SDS-PAGE. 4 * 55000, deglycosylated endogenous and recombinant tannases, SDS-PAGE | Blastobotrys adeninivorans |
| 3.1.1.20 | 61000 | - |
mature enzyme, calculated from the ORF | Blastobotrys adeninivorans |
| 3.1.1.20 | 80000 | - |
4 * 80000, denatured endogenous and recombinant tannases, SDS-PAGE. 4 * 55000, deglycosylated endogenous and recombinant tannases, SDS-PAGE | Blastobotrys adeninivorans |
| 3.1.1.20 | 320000 | - |
native endogenous and recombinant tannases, gel filtration | Blastobotrys adeninivorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Blastobotrys adeninivorans | B7VFD0 | - |
- |
| 3.1.1.20 | Blastobotrys adeninivorans LS3 | B7VFD0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.20 | endogenous and recombinant tannases purified by ion-exchange chromatography and gel filtration | Blastobotrys adeninivorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | catechin gallate + H2O | best substrate | Blastobotrys adeninivorans | gallic acid + catechin | - |
? | |
| 3.1.1.20 | catechin gallate + H2O | best substrate | Blastobotrys adeninivorans LS3 | gallic acid + catechin | - |
? | |
| 3.1.1.20 | epicatechin gallate + H2O | - |
Blastobotrys adeninivorans | gallic acid + epicatechin | - |
? | |
| 3.1.1.20 | epicatechin gallate + H2O | - |
Blastobotrys adeninivorans LS3 | gallic acid + epicatechin | - |
? | |
| 3.1.1.20 | epigallocatechin gallate + H2O | - |
Blastobotrys adeninivorans | gallic acid + epigallocatechin | - |
? | |
| 3.1.1.20 | epigallocatechin gallate + H2O | - |
Blastobotrys adeninivorans LS3 | gallic acid + epigallocatechin | - |
? | |
| 3.1.1.20 | gallic acid ethyl ester + H2O | - |
Blastobotrys adeninivorans | gallic acid + ethanol | - |
? | |
| 3.1.1.20 | gallic acid propyl ester + H2O | - |
Blastobotrys adeninivorans | gallic acid + propanol | - |
? | |
| 3.1.1.20 | gallocatechin gallate + H2O | - |
Blastobotrys adeninivorans | gallic acid + gallocatechin | - |
? | |
| 3.1.1.20 | gallocatechin gallate + H2O | - |
Blastobotrys adeninivorans LS3 | gallic acid + gallocatechin | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Blastobotrys adeninivorans | gallate + methanol | - |
? | |
| 3.1.1.20 | methyl gallate + H2O | - |
Blastobotrys adeninivorans LS3 | gallate + methanol | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Blastobotrys adeninivorans | gallic acid + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | homotetramer | 4 * 80000, denatured endogenous and recombinant tannases, SDS-PAGE. 4 * 55000, deglycosylated endogenous and recombinant tannases, SDS-PAGE | Blastobotrys adeninivorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | ATAN1 | - |
Blastobotrys adeninivorans |
| 3.1.1.20 | tannase | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 35 | 40 | endogenous and recombinant tannases | Blastobotrys adeninivorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 30 | 50 | endogenous and recombinant tannases | Blastobotrys adeninivorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 5.7 | 6 | endogenous and recombinant tannases | Blastobotrys adeninivorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 5 | 7 | endogenous and recombinant tannases | Blastobotrys adeninivorans |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.45 | - |
gallic acid | endogenous tannase | Blastobotrys adeninivorans | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.1.1.20 | Blastobotrys adeninivorans | in the presence of tannic acid, ATAN1 transcript declines after 12 h of incubation, by 24 h titres of the ATAN1 transcript drop below the detection limit | down |
| 3.1.1.20 | Blastobotrys adeninivorans | ATAN1 is expressed only in the presence of tannic acid or gallic acid. Transcripts are first detected 2 h after the shift from glucose to tannic acid medium, levels then increase, reaching a maximum after 4-12 h. ATAN1 transcript is detectable within 30 min in the presence of gallic acid | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | physiological function | enzyme titre with the recombinant strain (390 U/l) is approximately 10times higher than that in the control strain without the TEF1 promoter-ATAN1 gene expression cassette. The Atan1 protein contains at positions 210-214 the canonical Gly-X-Ser-X-Gly motif found in the serine hydrolases as the catalytic triad for nucleophilic serine | Blastobotrys adeninivorans |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | 0.064 | - |
Tannic acid | recombinant tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 0.078 | - |
Tannic acid | endogenous tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 0.112 | - |
methyl gallate | recombinant tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
| 3.1.1.20 | 0.127 | - |
methyl gallate | endogenous tannase, at 30°C, pH 6.0 | Blastobotrys adeninivorans | |
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